Cloning, expression analysis and functional marker development methods of millet SiARGOS gene

A technology of cloning method and analysis method, applied in the fields of cloning, expression analysis and functional marker development

Pending Publication Date: 2017-12-05
山西省农业科学院谷子研究所
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Problems solved by technology

At present, there is no report on the study of the millet ARGOS gene and its promoter

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  • Cloning, expression analysis and functional marker development methods of millet SiARGOS gene
  • Cloning, expression analysis and functional marker development methods of millet SiARGOS gene
  • Cloning, expression analysis and functional marker development methods of millet SiARGOS gene

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Embodiment Construction

[0023] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0024] 1 Materials and methods

[0025] 1.1 Plant material, planting and sampling

[0026]Millet varieties used for gene and its promoter cloning: Jin 29A and K186, planting method: one row per material planted in the experimental field, row length 2.5 meters, row spacing 33cm, sampling period: young leaves at jointing stage; used for gene SNP and single A total of 19 millet varieties were analyzed for ploidy: Aininghuang, Shawan millet, Miyou, Hebei Shilixiang, Zhang 8311-13, ISE-4, Lengsheng-1, Pinzi 15, Qianchuanzi, Changnong 35 , Shandong-5, Mengxuan 5084, Changsui 7, Heimaogu, Jilin Jingu No. 2, Mengjingu No. 1, Shi 96355, Gao 146A, planting method and sampling period are the same as above; millet used for phenotypic identification and gene expression Variety: Jin 29A, K186 and 'Jin 29AxK186' hybrid F ...

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Abstract

The invention discloses cloning, expression analysis and functional marker development methods of millet SiARGOS gene. Millet SiARGOS gene coding region and promoter sequence are cloned. It is found through sequence analysis that SiARGOS gene open reading frame 342bp codes 114 amino acids and the protein has crop ARGOS protein typical leucine-rich domain and promoter region 2109bp and contains various plant hormone regulation-related components such as auxin, ethene, jasmonic acid, gibberellins, etc. Phylogenetic analysis shows that millet ARGOS protein has the highest homology as corn ARGOS protein. It is found through expression analysis that SiARGOS gene has great differences in expression level of Jin 29A, K186 and their hybrid F1 roots. It is found through SiARGOS gene allelic variation analysis that one SNP exists in SiARGOS gene ORF, 19 SNPs and 2 InDels exist in the promoter region and four haplotypes are found. Three functional markers have been developed, including one CAPS marker and two SSR markers.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for cloning, expression analysis and functional marker development of millet SiARGOS gene. Background technique [0002] Crop yield traits are closely related to plant organ size. In addition to being affected by external environmental conditions such as light, temperature, nutrition, and hormones, plant organ size is mainly regulated by genetic factors such as gene expression and signal transduction. Studies suggest that the total number of cells Growth and unicellular volume increase are two continuous and related processes that control the size of plant organs. The size of plant organs varies significantly among different species but is relatively consistent among individuals within a species, indicating that cell division is regulated during organ development. Strict control of genetic material. [0003] Predecessors have cloned and studied many related gene...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/10C12Q1/68
CPCC07K14/415C12Q1/6895C12Q2600/156
Inventor 王军王智兰杜晓芬杨慧卿王玉文郭二虎袁峰田岗李会霞刘鑫张林义彭书忠
Owner 山西省农业科学院谷子研究所
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