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A method for shortening the sprouting and seedling time of eggplant

A seed and eggplant technology is applied in the field of shortening the time of eggplant germination and seedling formation, which can solve the problems of complexity and low seedling growth rate of eggplant tissue culture, and achieves the effects of simple method, saving the time of seed fruit ripening, and high seedling growth rate.

Inactive Publication Date: 2020-06-12
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the main direction of research on eggplant biotechnology breeding and variety selection is the tissue culture and plant regeneration of various organs of eggplant, including the regeneration of seedling explant organs such as stems, leaves, leaf stems, cotyledons, roots, and hypocotyls. Plants, these studies provide a way for biological breeding and improve eggplant breeding efficiency, but eggplant tissue culture generally has a relatively low seedling rate, the highest seedling rate is about 30%, and some explant culture techniques are more complicated ( Isouard et al., 1979; Gleddie et al., 1983; Sharma and Rajam, 1995; Magioli et al., 1998)

Method used

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  • A method for shortening the sprouting and seedling time of eggplant
  • A method for shortening the sprouting and seedling time of eggplant
  • A method for shortening the sprouting and seedling time of eggplant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1, Yuanza No. 5 immature seeds are induced to become seedlings

[0058] (1), Yuan Za No. 5 (purchased from Zhongshu Seed Industry Technology (Beijing) Co., Ltd.) immature seeds induced seedlings

[0059] 1. Determination of time to collect immature seeds and fruit

[0060] Collect the fruit of Yuanza No. 5 eggplant after flowering 10, 20, and 30 days from the robust plants growing under natural conditions. The fruit requires no pests and diseases on the surface.

[0061] 2. Collection and processing of immature seeds

[0062] (1) Pericarp disinfection ① carry out fruit surface disinfection with 75% (volume percentage content) alcohol, disinfection time is 30sec; ② soak 15min with 6.5% (mass percentage content) sodium hypochlorite solution; ③ wash with sterile water for 3 5 minutes each time, and then blotted dry with sterile paper for later use.

[0063] (2) Collect immature seeds on the ultra-clean workbench, cut the sterilized fruit with a scalpel, use t...

Embodiment 2

[0075] Embodiment 2, Changza No. 8 immature seeds induce seedling formation

[0076] (1) Acquisition of immature seeds of Changza No. 8 (purchased from Zhongshu Seed Industry Technology (Beijing) Co., Ltd.)

[0077] 1. Determination of time to collect immature seeds and fruit

[0078] Collect the fruit of Changza No. 8 eggplant after 10, 20, and 30 days of flowering on robust plants growing under natural conditions, and the fruit requires no pests and diseases on the surface.

[0079] 2. Collection and processing of immature seeds

[0080] (1) Pericarp disinfection: 1. carry out fruit surface disinfection with 75% (volume percentage content) alcohol, disinfection time is 30sec; 2. soak 15min with 6.5% (mass percentage content) sodium hypochlorite solution; 3. wash with sterile water 3 times, 5min each time, then blot dry with sterile paper for later use.

[0081] (2) Harvesting of immature seeds: on the ultra-clean workbench, cut the sterilized fruit with a scalpel, use twe...

Embodiment 3

[0092] Embodiment 3, different hormones in the induction medium affect the seedling induction of immature seeds (No. 5 garden miscellaneous)

[0093] (1), Yuan Za No. 5 immature seeds induced seedlings

[0094] 1. Collection and processing of immature seeds

[0095] Collect the fruit of Yuan Za No. 5 eggplant after flowering 20 days on the vigorous plant growing under natural conditions, and the fruit requires no damage by diseases and insect pests on the surface.

[0096] (1) Pericarp disinfection: 1. carry out fruit surface disinfection with 75% (volume percentage content) alcohol, disinfection time is 30sec; 2. soak 15min with 6.5% (mass percentage content) sodium hypochlorite solution; 3. wash with sterile water 3 times, 5min each time, then blot dry with sterile paper for later use.

[0097] (2) Harvesting of immature seeds: on the ultra-clean workbench, cut the sterilized fruit with a scalpel, use tweezers to remove the seeds with complete shape and not cut by the scal...

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Abstract

The invention provides a method capable of shortening the germination and seedling formation time of eggplants. The method is characterized in that part of seed coat and cotyledon parts of immature seeds in fruits 20 days after the flowering of the eggplants is cut off under a sterile condition, and then the seeds are transferred into a germination and rooting induction culture medium. When the method and the applicable culture medium of the method are applied to eggplant genetic breeding, the next-generation germination and seedling formation time from eggplant flowering to seed maturity can be greatly shortened by 30-40 days, and eggplant breeding is accelerated. In addition, the method is a new eggplant explant cultivation method, the method can effectively save eggplant offspring with insufficient maturity time, the seeds in immature fruits can be induced to form seedlings so as to obtain offspring plants, and reliable technical support is provided for the acceleration of genetic breeding and genetic researches of solanaceous vegetables of China.

Description

technical field [0001] The invention belongs to the field of plant cell engineering, and in particular relates to a method for shortening the sprouting time of eggplants. Background technique [0002] Eggplant, scientific name Solanum melongena L, is one of the main vegetable varieties produced and consumed in my country. In 2014, the eggplant production area in my country was 802,306ha, and the output reached 29,490,095t (FAO, 2014), accounting for 59% of the world's total eggplant production. [0003] Eggplant is a temperature-loving crop. The seeds of eggplant mature later than the fruit, and it takes longer for the seeds to mature. Under normal circumstances, eggplant seeds can germinate 40 days after flowering and pollination, but it takes 50-60 days to fully mature (Yu Fengjuan, 2015). In addition, the germination rate of eggplant seeds harvested 50-55 days after flowering is generally low (Gao Weiheng et al., 1991), and the newly harvested eggplant seeds generally h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01C1/00A01G7/06A01G22/00
Inventor 陈钰辉连勇刘富中张映
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI