THSD7A (Thrombospondin type-1 domain-containing 7A) gene sequence and application thereof to expression change detection and coronary heart disease prediction

A technology of DNA sequence and coronary heart disease, which is applied in the detection of THSD7A gene sequence and expression changes and its application in the prediction of coronary heart disease, can solve the problems of lack of effective methods for susceptibility genes and insufficient explanation of the genetic basis of coronary heart disease, etc. To achieve the effect of reducing the incidence of

Active Publication Date: 2017-12-08
田小利 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the other hand, only 6 of the discovered loci are based on the research findings of the Chinese Han population, which is far from enough to explain the genetic basis of coronary heart disease in the Chinese Han population
In addition, most of the existing studies only focus on the related loci, and there is no effective method for identifying the susceptibility genes referred to by the related loci.

Method used

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  • THSD7A (Thrombospondin type-1 domain-containing 7A) gene sequence and application thereof to expression change detection and coronary heart disease prediction
  • THSD7A (Thrombospondin type-1 domain-containing 7A) gene sequence and application thereof to expression change detection and coronary heart disease prediction
  • THSD7A (Thrombospondin type-1 domain-containing 7A) gene sequence and application thereof to expression change detection and coronary heart disease prediction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Example 1: Genome-wide screening of coronary heart disease susceptibility loci:

[0071] Experimental population

[0072] In this embodiment, the patients with coronary heart disease (coronary angiography positive), that is, the samples and case data of the coronary heart disease case group came from the General Hospital of the Chinese People's Liberation Army, and the samples and data of the control group came from the Beijing Physical Examination Center. All samples from the population participating in the experiment were notified in writing, and informed consent was signed by themselves or their family members. The experimental operation followed the ethical principles stipulated in the "Declaration of Helsinki" and was reviewed and approved by the Peking University Ethics Committee.

[0073] Among them, the selection criteria for samples from the coronary heart disease case group meet one of the following three conditions:

[0074] (1) Previous medical history of ...

Embodiment 2

[0298] Multi-center verification of embodiment 2 rs17165136 locus

[0299] In order to further verify the rs17165136 single nucleotide polymorphism site in Example 1, this example will carry out multi-center verification on the above site. The method used for verification is still the combination of LCR and direct sequencing. The specific operation is as follows:

[0300] research population

[0301] The samples and data of patients with coronary heart disease (positive coronary angiography) used in the experiment came from the Affiliated Hospital of Harbin Medical University, China-Japan Friendship Hospital, Fuwai Hospital, and the First Affiliated Hospital of Zhengzhou University. The control samples and data came from the physical examination centers in Northeast and North regions that matched the regions of the cases; the case samples and data verified by the southern population came from the First People's Hospital of Jiangsu Province, the First People's Hospital of Nin...

Embodiment 3

[0314] Example 3 Identification of susceptibility genes

[0315] Unlike many SNP sites found in GWAS located in gene deserts, the rs17165136 site identified in this application is located in the THSD7A intron region, so the inventors hypothesized that the THSD7A gene may be a susceptibility gene for coronary heart disease. And verify it through this embodiment.

[0316] research population

[0317] The population used for eQTL analysis of THSD7A gene came from the Beijing Physical Examination Center. All samples from the population participating in the experiment were notified in writing, and informed consent was signed by themselves or their family members. The experimental operation followed the ethical principles stipulated in the "Declaration of Helsinki" and was reviewed and approved by the Peking University Ethics Committee.

[0318] cell culture

[0319] Human embryonic kidney cell line HEK293A was preserved by this experiment; primary human umbilical vein endothelial...

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Abstract

The invention relates to the technical field of biology. On one hand, the invention relates to a single nucleotide polymorphism (SNP) site of a coronary heart disease susceptible gene THSD7A (Thrombospondin type-1 domain-containing 7A) and a corresponding composition and a corresponding kit which are used for detecting the SNP site; the invention especially relates to application of a nucleic acid affinity ligand of the SNP site to preparation of a composition which is used for detecting, screening or predicting coronary heart disease susceptibility of Chinese Han population. On the other hand, the invention relates to a corresponding composition and a corresponding kit which are used for detecting the THSD7A gene and further relates to application of a nucleic acid affinity ligand of the THSD7A gene to preparation of the composition for detecting, screening or predicting the coronary heart disease susceptibility of Chinese Han population. The invention provides new further scientific evidence for researching and developing targeted coronary heart disease gene therapy, and a new clue is provided for finding a new coronary heart disease molecule mechanism and developing a new medicine target point and individualized treatment of a coronary heart disease.

Description

[0001] This application is a divisional application of a patent application with an application date of January 21, 2015, an application number of 201510029413.7, and an invention title of "THSD7A gene sequence and expression change detection and its application in coronary heart disease prediction". technical field [0002] The invention relates to the field of biotechnology, in particular to a single nucleotide polymorphism (single nucleotide polymorphism, SNP) site on the THSD7A gene associated with susceptibility to coronary heart disease, and a corresponding combination for detecting the SNP site The invention relates to a substance and a kit, in particular to the application of the nucleic acid affinity ligand of the SNP site in the preparation of a composition for detecting, screening or predicting the susceptibility of coronary heart disease in Han population. Background technique [0003] Coronary heart disease, also known as coronary heart disease, is an ischemic he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q1/6883C12Q2600/156
Inventor 田小利李扬
Owner 田小利
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