Unlock instant, AI-driven research and patent intelligence for your innovation.

Synergistic Degradation of Polyethylene Plastic Using Two Bacteria

A polyethylene plastic and synergistic degradation technology, applied in the field of solid waste biodegradation, can solve the problems of low degradation efficiency and long degradation time of polyethylene plastic, achieve significant economic and environmental protection benefits, shorten the degradation cycle, and high degradation efficiency Effect

Active Publication Date: 2020-05-05
CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practice, it was found that although these bacteria can degrade polyethylene, the degradation efficiency of polyethylene plastic is very low, and the degradation time is very long

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Synergistic Degradation of Polyethylene Plastic Using Two Bacteria
  • Synergistic Degradation of Polyethylene Plastic Using Two Bacteria
  • Synergistic Degradation of Polyethylene Plastic Using Two Bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The method for utilizing two kinds of bacteria in this embodiment to synergistically degrade polyethylene plastics comprises the following steps:

[0047] a. After 0.22g polyethylene plastic is sterilized, it is placed on the 250ml beef extract peptone liquid medium with a pH of 7.0. The beef extract peptone liquid medium uses deionized water as a solvent, and includes the following groups according to concentration Subdivided: beef extract 30g / L, peptone 15g / L and sodium chloride 15g / L;

[0048] b. Inoculate Pseudomonas fluorescens and Pseudomonas aeruginosa in a ratio of 1:2 on the medium of step a, the total inoculation amount is 10% of the volume of the medium, and then culture at a temperature of 35°C. The medium was changed every 15 days, and two bacteria were used to synergistically degrade polyethylene plastic.

[0049] In the present embodiment, the sterilization method of polyethylene plastic comprises the steps:

[0050] (1) After soaking the polyethylene p...

Embodiment 2

[0060] The method for utilizing two kinds of bacteria in this embodiment to synergistically degrade polyethylene plastics comprises the following steps:

[0061] a. After 0.21g polyethylene plastic is sterilized, it is placed on the 250ml beef extract peptone liquid medium with a pH of 7.0. The beef extract peptone liquid medium uses deionized water as a solvent, and includes the following groups according to concentration Subdivided: beef extract 28g / L, peptone 12g / L and sodium chloride 12g / L;

[0062] b. Inoculate Pseudomonas fluorescens and Pseudomonas aeruginosa in a ratio of 1:1 on the medium of step a, the total inoculation amount is 8% of the volume of the medium, and then cultured at a temperature of 30°C. The medium was changed every 15 days, and two bacteria were used to synergistically degrade polyethylene plastic.

[0063] In this example, the same method as in Example 1 was used to sterilize polyethylene plastic and obtain Pseudomonas fluorescens and Pseudomonas ...

Embodiment 3

[0065] The method for utilizing two kinds of bacteria in this embodiment to synergistically degrade polyethylene plastics comprises the following steps:

[0066] a. After 0.23g polyethylene plastic is sterilized, it is placed on the 250ml beef extract peptone liquid medium with a pH of 7.2. The beef extract peptone liquid medium uses deionized water as a solvent, and includes the following groups by concentration Subdivided: beef extract 32g / L, peptone 18g / L and sodium chloride 18g / L;

[0067] b. Inoculate Pseudomonas fluorescens and Pseudomonas aeruginosa in a ratio of 1:3 on the medium in step a, the total inoculum amount is 10% of the volume of the medium, and then culture at a temperature of 37°C. The medium was changed every 15 days, and two bacteria were used to synergistically degrade polyethylene plastic.

[0068] In this example, the same method as in Example 1 was used to sterilize polyethylene plastic and obtain Pseudomonas fluorescens and Pseudomonas aeruginosa fo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for performing synergetic degradation on polyethylene plastic through two bacteria. The method includes the following steps that a, after the polyethylene plastic is subjected to sterilization treatment, the polyethylene plastic is placed in a liquid culture medium; and b, fluorescent pseudomonas and pseudomonas aeruginosa are inoculated on the liquid culture medium in the step a in a mixed manner, then the fluorescent pseudomonas and the pseudomonas aeruginosa are placed in a constant temperature condition to be cultivated, and the two bacteria are utilized to perform synergetic degradation on the polyethylene plastic. According to the method, the fluorescent pseudomonas and the pseudomonas aeruginosa adopted for the method have the polyethylene degrading capacity, the polyethylene plastic can be effectively eroded and degraded, not only can enzyme oxidizing and degrading polyethylene be generated in a fermentation manner, but also the fluorescent pseudomonas and the pseudomonas aeruginosa can be mutually promoted and synergetically play a degrading effect on the polyethylene plastic, and under the same condition, the degrading effect caused when the fluorescent pseudomonas and the pseudomonas aeruginosa are inoculated on the polyethylene plastic in the mixed manner is greatly superior to the degrading effect caused when the fluorescent pseudomonas or the pseudomonas aeruginosa is singularly inoculated.

Description

technical field [0001] The invention relates to the technical field of solid waste biodegradation, in particular to a method for synergistically degrading polyethylene plastics by using two kinds of bacteria. Background technique [0002] Plastic waste is accumulating in the environment at a rate of 40 million tons per year, which is the main source of environmental "white pollution" and has brought about very serious ecological and environmental pollution problems. [0003] Polyethylene-[CH 2 -CH 2 ]n- is one of the most commonly used polymer materials in daily life, and it is also one of the plastics with the largest output and consumption in the world. All aspects of the economy and human life. Due to the long chain and hydrophobic macromolecular structure of polyethylene, polyethylene plastic has a long degradation cycle, and the degradation process in the natural environment is extremely slow. [0004] For a long time, researchers from various countries have been wo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A62D3/02A62D101/20
CPCA62D3/02A62D2101/20
Inventor 王侨邱会东秦正山张小敏
Owner CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY