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Concentration method of coenzyme I

A technology of coenzyme and concentrated solution, applied in the field of concentration of coenzyme I, can solve the problems of not meeting the requirements of the concentration volume of biological products, the concentration limit is only 20-30%, the destruction of active components, etc., and achieves easy operation and energy saving. , the effect of increasing the concentration limit

Inactive Publication Date: 2017-12-15
开封康诺药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, membrane concentration is widely used now, but its disadvantage is that the concentration limit is only 20-30%, which cannot meet the concentration volume requirements of biological products
Traditional evaporation and concentration is only suitable for the preparation of some heat-resistant enzymes and small molecule biochemical drugs; it is not suitable for temperature-sensitive biological products, which may easily cause damage to active ingredients

Method used

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  • Concentration method of coenzyme I

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A new enrichment technology of coenzyme I, comprising the following steps:

[0023] S1. Sample solution preparation: Coenzyme I was dissolved in a solvent containing 0.5% acetic acid and 1.5% methanol to obtain crude coenzyme I solution A. Wherein, the volume ratio of coenzyme I to solvent is 0.5:60.

[0024] S2. Nanofiltration concentration: transfer the solution A into the receiving tank of the nanofiltration concentration equipment, turn on the nanofiltration concentration equipment, the working pressure is 6bar, input the solution A into the nanofiltration membrane tube with a molecular weight cut-off of 200D, and continuously circulate and concentrate Concentrate B was obtained. Wherein, the volume of the obtained concentrated solution B is 1 / 7 of the solution A.

[0025] S3. Ultra-low temperature concentration: the concentrated solution B was concentrated under reduced pressure with a rotary evaporator, and the parameters were set: vacuum degree 5mbar, temperatu...

Embodiment 2

[0027] A new enrichment technology of coenzyme I, comprising the following steps:

[0028] S1. Sample solution preparation: Coenzyme I was dissolved in a solvent containing 1.0% acetic acid and 2.5% methanol to obtain crude coenzyme I solution A. Among them, the volume ratio of coenzyme I to solvent is 0.7:75.

[0029] S2. Nanofiltration concentration: transfer solution A into the receiving barrel of the nanofiltration concentration equipment, turn on the nanofiltration concentration equipment, set the working pressure to 6.5 bar, and input the solution A into the nanofiltration membrane tube with a molecular weight cut-off of 200D, Concentrated solution B is obtained by continuous circulation and concentration. Wherein, the volume of the obtained concentrated solution B is 1 / 6 of the solution A.

[0030] S3. Ultra-low temperature concentration: the concentrated solution B was concentrated under reduced pressure with a rotary evaporator, and the parameters were set: vacuum d...

Embodiment 3

[0032] A new enrichment technology of coenzyme I, comprising the following steps:

[0033] S1. Sample solution preparation: Coenzyme I was dissolved in a solvent containing 2.1% acetic acid and 3.5% methanol to obtain crude coenzyme I solution A. Wherein, the volume ratio of coenzyme I to solvent is 0.65:79.

[0034] S2. Nanofiltration concentration: transfer solution A into the receiving tank of the nanofiltration concentration equipment, turn on the nanofiltration concentration equipment, set the working pressure to 7.5 bar, and input solution A into the nanofiltration membrane tube with a molecular weight cut-off of 300D, Concentrated solution B is obtained by continuous circulation and concentration. Wherein, the volume of the obtained concentrated solution B is 1 / 5 of the solution A.

[0035] S3. Ultra-low temperature concentration: the concentrated solution B was concentrated under reduced pressure with a rotary evaporator, and the parameters were set: vacuum degree 7m...

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Abstract

The invention belongs to and relates to the field of extraction of coenzyme I, and provides a concentration method of coenzyme I. The concentration method of the coenzyme I comprises the following steps: S1, preparing a sample liquid: dissolving the coenzyme I into a solvent containing acetic acid and methanol and mixing uniformly to obtain a coenzyme I crude product solution; S2, performing nanofiltration and concentration: transferring the coenzyme I crude product solution into a liquid receiving barrel of nanofiltration and concentration equipment, staring the nanofiltration and concentration equipment, inputting the coenzyme I crude product solution into a nanofiltration membrane tube, and continuously circulating concentrated liquid; and S3, performing ultralow temperature concentration: performing ultralow temperature concentration on the concentrated liquid to obtain the final concentrated liquid. By the concentration method of the coenzyme I, the concentration limit of the coenzyme I crude product solution is greatly increased, and the coenzyme I crude product solution is concentrated into a certain volume required before freeze drying; the problem of strict requirement of production of the coenzyme I on the temperature is solved, and the material components maintain stable and are not degraded or oxidized; meanwhile, the organic solvent residues in the raw materials of the coenzyme I are greatly reduced.

Description

technical field [0001] The invention relates to the field of coenzyme I extraction, in particular to a method for concentrating coenzyme I. Background technique [0002] Coenzyme I (NAD), chemically named nicotinamide adenine dinucleotide or nicotinic acid diphosphate, exists in two states of oxidized (NAD+) and reduced (NADH) in mammals, and is an important redox reaction in the human body. Coenzymes participate in various physiological activities such as cell material metabolism, energy synthesis, and cell DNA repair, and play an important role in the body's immunity. In a healthy state, the concentration of nicotinamide adenine dinucleotide in the human body is stable to maintain the normal functions of various cells. The concentration of nicotinamide adenine dinucleotide in the body determines the process and degree of cell aging, and the decrease in concentration will accelerate the process of cell aging. [0003] Coenzyme I is unstable to heat and is very sensitive t...

Claims

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Application Information

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IPC IPC(8): C07H1/06C07H19/207
CPCC07H1/06C07H19/207
Inventor 金永红郎巧洁李映峤
Owner 开封康诺药业有限公司