Glass needle tip nanopore filling device based on vacuum method and using method thereof

A nanopore and vacuum technology, applied in the fields of life science and biomolecule detection, can solve the problems of difficult discharge of needle tip bubbles and difficulty in filling glass needle tip nanopores, so that the properties of the solution will not change, and the filling rate and usage rate will be improved. , the effect of high success rate

Active Publication Date: 2020-12-29
CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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  • Application Information

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Problems solved by technology

[0004] In view of this, in order to solve the problem that it is difficult to fill the entire glass needle tip nanopore and the needle tip bubbles are difficult to discharge in the glass needle tip nanopore filling method that uses a microsyringe to inject filling liquid into the microtube at present, the present invention provides a vacuum based Glass needle-tip nanopore filling device and using method thereof

Method used

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  • Glass needle tip nanopore filling device based on vacuum method and using method thereof
  • Glass needle tip nanopore filling device based on vacuum method and using method thereof
  • Glass needle tip nanopore filling device based on vacuum method and using method thereof

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Embodiment 1

[0034] The first step, pre-filling preparation

[0035]The quartz capillary with an outer diameter of 1.0 mm and an inner diameter of 0.7 mm was treated with piranha washing solution (volume ratio, 98% H2SO4: 30% H2O2 = 3: 1) for 30 minutes in a heated state, and then ultrasonically cleaned and rinsed with deionized water , blow dry with nitrogen, and dry at 60°C for 30 minutes, cool to room temperature, dry and store for later use; the glass tube 103 with a tip diameter of about 30 nm and a glass nanopore tip drawn by a P-2000 laser drawing machine , the glass tube 103 needle tip micrograph after drawing is shown in Figure 4 , using a micro-syringe to inject the required 1mol / L LiCl (pH=8) electrolyte solution 104 into the centrifuge tube 102; then, the glass tube 103 is fixed on the holder 101, and the upper part 203 of the needle tip does not exceed the liquid storage tank 204 bottom 201 , the holder 101 is inserted into the matching 1.5ml microcentrifuge tube 102 , and t...

Embodiment 2

[0041] The difference between embodiment 2 and embodiment 1 is that the solution 104 is replaced in the first step, and the specification of the quartz capillary is 1.0 mm in outer diameter and 0.5 mm in inner diameter. The diameter of the glass tip nanopore filled with the solution 104 is about 25nm, and the tip is fixed on the holder 101. The upper part 203 of the tip does not exceed the bottom 201 of the liquid tank 204, and the holder 101 is inserted into the matching 1.5ml microcentrifuge tube 102 , and dip the needle tip into the electrolyte solution 104 (1 nM λDNA, 1 mol / LLiCl, pH=8) containing the DNA sample.

[0042] In the third step, seal the interface between the holder 101 and the centrifuge tube 102 with a parafilm, then place the centrifuge tube 102 on the shelf, put it in a vacuum desiccator, extract the vacuum, and observe the glass tube 103 in the process of vacuum removing air bubbles. The solution 104 inside overflows upwards, and the overflowing solution 1...

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Abstract

The invention belongs to the technical field of life science and biomolecule detection, and relates to a needle tip nanopore filling device. The device comprises a centrifuge pipe and a clamp holder clamped on the centrifuge pipe in a sealed mode, wherein a liquid containing tank is integrally formed at the top of the clamp holder; and the clamp holder is in a up-down through mode, and a glass pipe with a needle tip nanopore structure is arranged in a through hole in the middle part of the clamp holder in an inserted mode. The problems that a whole glass needle tip nanopore is difficult to fill and needle tip bubbles are difficult to discharge in a glass needle tip nanopore filling method for injecting filling liquid into a micropipe by adopting a microinjector in the prior art are solved.

Description

technical field [0001] The invention belongs to the technical fields of life science and biomolecular detection, and relates to a glass needle tip nanopore filling device, in particular to a glass needle tip nanopore filling device based on a vacuum method and a use method thereof. Background technique [0002] Single-molecule sensors based on nanopore technology have been used in the preparation of biological and chemical sensors, detection and screening of biomolecules, and DNA sequencing. The needle-tip nanopore technology is a kind of solid-state nanopore. Compared with the traditional solid-film nanopore, it has obvious advantages such as easy fabrication, high signal-to-noise ratio, and low cost. At present, the filling method of glass needle-tip nanopores is mainly to use a micro-syringe to inject into the microtube, and then flick the needle tip to remove air bubbles, but this method cannot achieve the filling of the entire glass needle-tip nanopore. And because the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N33/00
CPCG01N1/28G01N33/00
Inventor 谢婉谊王德强何石轩方绍熙梁丽媛周大明殷博华
Owner CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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