Culture medium and culture method for promoting synechocystis sp. to secrete amino acids and algal strain

Abstract

The invention relates to a culture medium for promoting synechocystis sp. PCC 6803 to secrete amino acids. The culture medium is a basal culture medium into which glucose with the concentration of 3.5-21g / L is added, and the concentration of the NaNO3 is 6-12 g / L. The invention further relates to a culture method for promoting the synechocystis sp. PCC 6803 to secrete the amino acids. The culture method comprises the step of using the culture medium for culturing the synechocystis sp. PCC 6803 so as to reach the condition of secreting the amino acids. The invention further relates to an algal strain obtained through genetic modification of the amino acid secreting synechocystis sp. PCC 6803. After use of the culture medium, the culture method and the algal strain, biological denitrationcan be performed by virtue of the synechocystis sp. PCC 6803, and inorganic nitrogen is converted into organic nitrogen so as to produce the amino acids. Under better conditions, after application of the synechocystis sp. PCC 6803 cultured by the culture method provided by the invention, the daily free amino acid output reaches 52.94 mg / L / d plus or minus 1.50 mg / L / d, and the daily sodium nitrate consumption reaches 0.51 g / L / d plus or minus 0.016 g / L / d.

Description

Technical field [0001] The present invention relates to the application field of microalgae, and more particularly, to a culture medium and a culture method for promoting the secretion of amino acids of Synechocystis PCC6803, and also to a genetically modified algae strain of Synechocystis PCC 6803 that secretes amino acids. Background technique [0002] Cyanobacteria is a kind of "cell factory" that can produce high value-added products. Genomics, transcriptomics, and metabolomics methods have been widely used in research related to cyanobacteria genetic modification. [0003] Today, when fossil fuels are used in large quantities, the issue of air pollution has attracted much attention. The gas released by the burning of fossil fuels contains nitrogen oxides (NO x ), sulfur oxides (SO x ) And other harmful gases. Nitric oxide (NO) is the NO in industrial exhaust gas x The main component of it can be slowly oxidized to NO by oxygen in the air 2 , Further causing acid rain, smog, a...

AI Technical Summary

Problems solved by technology

The traditional denitrification method mainly reacts ammonia and nitrogen oxides to generate nitrogen gas, without producing any useful products, and will cause secondary pollution due to excessive ammonia

There is no report on the production of amino acids with Synechocystis PCC 6803

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