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Method for extraction of lutein from marigold

A technology of lutein and marigold, which is applied in the production and fermentation of bulk chemicals, can solve the problems of low modification rate of lutein ester, low amount of free lutein, and low extraction rate, so as to shorten the enzymatic hydrolysis time, Increased permeability, high content effect

Inactive Publication Date: 2017-12-15
兰溪市顺光园艺技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When using this method to extract lutein, the modification rate of lutein ester is not high, the amount of free lutein obtained by saponification is small, and the extraction rate is not high, which will cause waste of lutein, low yield and high cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The method for extracting lutein from marigold, the specific steps are:

[0021] 1) Fermentation: Dissolve Angel yeast powder in water, add crushed and sieved marigold granules and active polypeptide, mix well, the amount of Angel yeast powder added is 0.4~0.8% of the mass of marigold, yeast powder: water The mass volume ratio is: 1g: (200~250) mL. The amount of active short peptide added is 0.1-0.3 times of the mass of yeast powder, and the amino acid sequence is QSASCTSLGCYCSKFCGTAGCGCYCLHCSR. Air-isolated fermentation, the fermentation temperature is 38~45℃, the fermentation time is 6~8 days, and it is dried after fermentation;

[0022] 2) Enzymatic hydrolysis: add organic solvent and cellulase to the fermented marigold granules, conduct infrared temperature-controlled enzymatic hydrolysis, and inactivate the enzyme after enzymatic hydrolysis. The concentration of cellulase is 6000~8000U / g, the substrate concentration is 10~20%, and the enzymatic hydrolysis time is...

Embodiment 2

[0026] The method for extracting lutein from marigold, the specific steps are:

[0027] 1) Fermentation: Dissolve Angel yeast powder in water, add crushed and sieved marigold granules and active peptides, mix evenly, the amount of Angel yeast powder added is 0.6% of the mass of marigold, yeast powder: the mass of water The volume ratio is: 1g: 200mL, and the water temperature is 40°C. The amount of the active short peptide added is 0.2 times the mass of yeast powder, and the amino acid sequence is QSASCTSLGCYCSKFCGTAGCGCYCLHCSR. Air-isolated fermentation, the fermentation temperature is 42°C, the fermentation time is 7 days, and it is dried after fermentation;

[0028] 2) Enzymatic hydrolysis: add organic solvent and cellulase to the fermented marigold granules, conduct infrared temperature-controlled enzymatic hydrolysis, and inactivate the enzyme after enzymatic hydrolysis. The concentration of cellulase is 7000U / g, the substrate concentration is 18%, and the enzymolysis t...

Embodiment 3

[0032] The method for extracting lutein from marigold, the specific steps are:

[0033] 1) Fermentation: Dissolve Angel yeast powder in water, add crushed and sieved marigold granules and active peptides, mix evenly, the amount of Angel yeast powder added is 0.7% of the mass of marigold, yeast powder: the mass of water The volume ratio is: 1g: 250mL, and the water temperature is 42°C. The added amount of active short peptide is 0.3 times of the quality of yeast powder, and the amino acid sequence is QSASCTSLGCYCSKFCGTAGCGCYCLHCSR. Air-isolated fermentation, the fermentation temperature is 45 ℃, the fermentation time is 7 days, and dried after fermentation. Through the fermentation of Angel yeast powder, the lutein ester can be converted into free lutein, instead of the saponification reaction, and the conversion rate of free lutein is high;

[0034] 2) Enzymatic hydrolysis: add organic solvent and cellulase to the fermented marigold granules, conduct infrared temperature-contr...

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PUM

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Abstract

The invention provides a method for extraction of lutein from marigold. The method includes: fermentation, enzymolysis, supercritical CO2 extraction and vacuum freeze drying. The method has the beneficial effects that: through fermentation of Angel yeast powder, lutein ester is converted into free lutein, saponification reaction is replaced, and the conversion rate of free lutein is high; the enzyme method destroys the cell structure completeness, more substances in cells can be exposed during extraction, thus increasing oil permeability. Enzymatic degradation of marigold flowers does cause isomerization of lutein, and the enzyme treated marigold powder has a high content of all-trans lutein; and the finally obtained lutein has high purity, high content of all-trans lutein and strong antioxidant properties, is green and healthy, and can be used for food, medicine, cosmetics fields.

Description

technical field [0001] The invention relates to the technical field of lutein extraction, in particular to a method for extracting lutein from marigolds. Background technique [0002] Lutein is the main antioxidant component of carotenoids in the retina of the eye tissue, which can prevent and delay vision degeneration, blindness or cataracts and other eye diseases induced by macular degeneration in the elderly. At the same time, it is an antioxidant with excellent performance, which can resist the cell and organ damage caused by oxygen free radicals in the human body, and prevent cardiovascular sclerosis, coronary heart disease and tumor diseases caused by body aging. It has been found in clinical medical research that eating more foods rich in lutein can greatly reduce the incidence of cancer. It can also eliminate or relieve "dry eye syndrome" caused by prolonged eye use (such as operating a computer, etc.). At the same time, when a sufficient amount of lutein is taken ...

Claims

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Application Information

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IPC IPC(8): C12P23/00
CPCC12P23/00Y02P20/54
Inventor 杨洋
Owner 兰溪市顺光园艺技术有限公司
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