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A method for screening broodfish with high glycogen content in long oyster

A technology of long oysters and high glycogen, applied in the field of genetic engineering and genetic breeding, can solve the problems of inability to understand genetic regulation mechanism, small number of markers, and no SNP markers, etc.

Active Publication Date: 2021-06-08
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

This kind of analysis is based on prior information, and the polymorphic sites are developed on the known functional genes. The relative number of markers is small, and the main effect sites may not be obtained, and the genetic regulation mechanism of the phenotype cannot be influenced. a comprehensive understanding
To date, there are no SNP markers developed based on the results of genome-wide association analyzes

Method used

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  • A method for screening broodfish with high glycogen content in long oyster
  • A method for screening broodfish with high glycogen content in long oyster
  • A method for screening broodfish with high glycogen content in long oyster

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Experimental program
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Embodiment 1

[0041] a) Collection of samples: In 2017, 288 individuals of the wild population of first-year-old oysters hatched at the same time in Jiaonan were collected, dissected, and the adductor muscle and remaining tissues were taken, which were quickly frozen in liquid nitrogen and stored at -80°C for later use.

[0042] b) Extraction of DNA: As described in the instructions, extract the genomic DNA of 288 samples, and measure the concentration of double-stranded DNA with Nanodrop2000 instrument at the same time, and dilute to 10-20ng / uL with sterile water;

[0043] c) SNP site genotype detection: Take the genomic DNA diluted in step b) as a template as described in the instructions, and use primers F and R for PCR amplification. The reaction system is as follows: Genomic DNA 1uL, primers F and R 0.2uL , PCR mix 5uL, sterile double distilled water 3.6uL;

[0044] The reaction procedure of PCR amplification is:

[0045]

[0046] The forward primer is F: 5'-TTTTCCACCAAGGTCCGA-3'; ...

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Abstract

The invention relates to a method for screening long oyster broodstock with high glycogen content and related SNP primer pairs. Through the genome-wide association analysis of 486 individuals, 39 SNP signals significantly associated with glycogen located on chromosome 6 of Oyster chinensis were identified. A corresponding detection method was developed for a SNP site at scaffold426_295, 537 bases in this region, and the nucleotide sequence of 500 bp upstream and downstream of the site is shown in SEQ ID No.1. At the same time, compared with CC genotype individuals, the glycogen content of TT genotype individuals at this site was significantly increased by 6.2%. The TT genotype broodstock at this site was screened to guide oyster breeding. The beneficial effect of the invention is that the genotype identification of parent shellfish can be carried out before seed breeding, the TT genotype parent shellfish at this site can be screened, and the glycogen content of progeny can be increased. The results of this study are based on the SNP markers obtained from the genome-wide association analysis of the wild population, and the effect is more stable.

Description

technical field [0001] The invention belongs to the field of genetic engineering and genetic breeding, and relates to a method for screening long oyster broodstock with high glycogen content and related SNP primer pairs. Background technique [0002] Oysters are an important marine aquatic resource and one of the most important marine cultured shellfish in the world. my country's oyster farming scale and output have ranked first in the world for many years, but the export price of oysters is low, making it difficult to enter the international high-end market. The comprehensive reasons are mainly due to the low nutritional quality of oysters. Therefore, improving the product quality of oysters is an important way to improve the economic benefits of oyster farming in my country and realize the transformation and upgrading of the industry from high-yield and low-efficiency to high-yield and high-efficiency models. Oysters are known as the milk of the sea, and the high content...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/166C12Q2600/172
Inventor 孟杰张国范李莉宋凯李春燕刘圣史瑞辉刘优丽
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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