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Method for screening maternal pacific oysters with high glycogen content and related SNP (Single Nucleotide Polymorphism) primer pair thereof

A technology of long oysters and high glycogen, applied in the fields of genetic engineering and genetic breeding, can solve the problems of inability to understand the genetic regulation mechanism and the small number of markers.

Pending Publication Date: 2017-12-15
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Application Information

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Problems solved by technology

However, this method is based on prior information and develops polymorphic sites on known functional genes. The relative number of markers is small, and the main effect sites may not be obtained, and the genetic regulation mechanism of the phenotype cannot be determined. have a comprehensive understanding

Method used

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  • Method for screening maternal pacific oysters with high glycogen content and related SNP (Single Nucleotide Polymorphism) primer pair thereof
  • Method for screening maternal pacific oysters with high glycogen content and related SNP (Single Nucleotide Polymorphism) primer pair thereof
  • Method for screening maternal pacific oysters with high glycogen content and related SNP (Single Nucleotide Polymorphism) primer pair thereof

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Experimental program
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Embodiment 1

[0037] a) Collection of samples: 288 individuals from wild populations in Jiaonan who hatched at the same time were collected, dissected, and the adductor muscle and remaining tissues were taken, which were quickly frozen with liquid nitrogen and stored at -80°C for later use.

[0038]b) Extraction of DNA: extract the genomic DNA of 288 samples and measure the concentration by ultraviolet absorptiometry, and dilute the genomic DNA to 10ng / uL with sterilized water according to the measured concentration;

[0039] c) SNP site genotype detection: take the genomic DNA diluted in step 1) as a template, and use primers F and R for PCR amplification. The reaction system is as follows: genomic DNA 1uL, primers F and R 0.2uL, PCR mix 5uL , sterile double distilled water 3.6uL;

[0040] The reaction procedure of PCR amplification is:

[0041]

[0042] After the completion of the PCR reaction, add 1 uL each of the saturated fluorescent dye Lcgreen and the internal standard, and annea...

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Abstract

The invention relates to a method for screening maternal pacific oysters with high glycogen content and a related SNP (Single Nucleotide Polymorphism) primer pair thereof. By virtue of genome-wide association study of 486 individuals, 67 SNP signals in significant correlation with glycogens located on chromosome 6 of the pacific oyster are identified. A corresponding detection method is developed aiming at one SNP site at the base scaffold 1243_53489 of the area, and the result shows that the SNP site can be simply and rapidly detected by utilizing the method. Meanwhile, the CC genotype individual at the site is compared with the TT genotype individual. CC genotype maternal oysters are screened to be used for guiding oyster breeding. A nucleotide sequence of the upstream / downstream 500kb of the pacific oysters at the site is shown as SEQ ID No.1. The method disclosed by the invention has the beneficial effects that the maternal oysters can be subjected to genotype identification before fry breeding, and the glycogen content of the offspring is improved. The study result is as follows: the obtained SNP marker has high reliability, and the effect is stable.

Description

technical field [0001] The invention belongs to the field of genetic engineering and genetic breeding, and relates to a pair of primers for screening SNP markers related to the glycogen content of long oyster and its application. Background technique [0002] Oysters are an important marine aquatic resource and one of the most important marine cultured shellfish in the world. my country's oyster farming scale and output have ranked first in the world for many years, but the average price of oyster exports is only 1 / 3 of other countries' oyster products. This shows that most oysters in my country can only be produced and sold by themselves, and it is difficult to enter the high-end market. Improving the economic benefits of oyster farming in my country, realizing the transformation and upgrading of the industry from high-yield and low-efficiency to high-yield and high-efficiency models, and improving the quality of oyster products are problems that need to be solved urgently...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 张国范李莉孟杰宋凯李春燕刘圣史瑞辉刘优丽
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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