Hybridization method of wild paddy rice

A hybrid method and wild rice technology, applied in the field of hybrid breeding, can solve the problems of large pollen amount, small anthers of cultivated rice, asynchronous flowering and pollen time, etc., and achieve the effects of large quantity and high success rate.

Active Publication Date: 2017-12-22
INST OF BOTANY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Although there are currently hybrid patents between cultivated rice and cultivated rice, and between cultivated rice and wild rice, there are large differences between wild rice and cultivated rice, which makes it impossible for many classical research methods in cultivated rice to mature. It is carried out smoothly in wild rice, and the differences related to hybridization are mainly reflected in the following points: a. Cultivated rice is mainly self-fertilized, while wild rice of different groups has different types of mating systems; b. Cultivated rice has small anthers and most of them The anthers of the varieties are less than 2mm, while the anthers of wild rice are large, some anthers can reach more than 6mm, and the amount of pollen is large, and the general method of killing males is difficult to be effective; c. The seeds of cultivated rice do not fall off after maturity, while the seeds of wild rice fall off after maturity; d .The flowering and pollination time of wild rice in different regions is not synchronized, which is slightly different from that of cultivated rice; e. The hybrid fertility of wild rice in different regions is unstable
It is these differences that make wild rice unsuitable for the hybridization method of cultivated rice, and the hybridization success rate is low

Method used

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  • Hybridization method of wild paddy rice
  • Hybridization method of wild paddy rice

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Embodiment 1, a kind of hybridization method of wild rice

[0043] The wild rice Oryza rufipogon was used as the female parent, and the wild rice Oryza nivara was used as the male parent as experimental materials for hybridization. Specific steps are as follows:

[0044] 1. Germination materials in batches

[0045] The wild rice O.rufipogon is a photosensitive material. The wild rice O.rufipogon needs at least about 80 days of vegetative growth before flowering under the stimulation of short days; the wild rice O.nivara is not sensitive to photoperiod, and the flowering period is 60- For 80 days, photoperiodic stimulation is not required. The flowering period of the wild rice O. rufipogon shall prevail. In order to ensure that the two flowering periods meet, the planting and processing shall be carried out as follows:

[0046] 1. Wild rice O.rufipogon

[0047] In Hainan, the wild rice O.rufipogon was planted from May to mid-June. After germination, it was transplant...

Embodiment 2

[0068] Embodiment 2, a kind of hybridization method of wild rice

[0069] The cultivated rice Nipponbare was used as the female parent, and the wild rice O. nivara was used as the male parent as experimental materials for hybridization. Specific steps are as follows:

[0070] 1. Germination materials in batches

[0071] Both the cultivated rice Nipponbare and the wild rice O. nivara are not sensitive to photoperiod, and the flowering period is 60-80 days without photoperiod stimulation. The flowering period of the wild rice O.nivara shall prevail. In order to ensure that the two flowering periods meet, the planting and processing shall be carried out as follows:

[0072] 1. Wild rice O.nivara

[0073] In Hainan, the wild rice O.nivara was planted in late July. After germination, it was transplanted into a paddy field. At the seedling stage, attention should be paid to insect and rodent control and routine management. During the flowering stage, the whole plant or more than ...

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Abstract

The invention discloses a hybridization method of wild paddy rice. A mature wild paddy rice hybridization technology is developed in the aspects of reasonably processing the different flowering phases of different materials, cautiously performing the castration operation, using different hybridization tools, controlling appropriate environmental conditions, identifying the hybridization and the like. The experiment proves that the success rate of the hybridization technology is high, more hybridized seeds can be obtained, the hybridization technology can be widely suitable for the hybridization requirements of different types of wild paddy rice and between the wild paddy rice and the cultivated paddy rice, the mining of genetic resources of the wild paddy rice can be facilitated, and contribution can be made to the diversified scientific research of the paddy rice.

Description

technical field [0001] The invention belongs to the field of hybrid breeding, and in particular relates to a method for hybridizing wild rice. Background technique [0002] Asian cultivated rice is one of the world's oldest crops and is the main source of food for nearly half the world's population. With the population boom and global climate change, people urgently need to constantly update the main varieties to meet the ever-changing and different needs. In the process of cultivating new varieties of crops, hybrid breeding, as the mainstream breeding method today, has developed a relatively complete technical route. It is hoped that hybrid offspring can not only inherit the superior agronomic traits of their parents, but also increase In terms of genetic differences among individuals, heterosis should be further applied to obtain better genetic breeding resources (Yuan Longping, 1997). In terms of basic scientific research, although new research methods using modern biote...

Claims

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/02A01H1/027
Inventor 耿牧帆孟庆霖徐迅葛颂
Owner INST OF BOTANY CHINESE ACAD OF SCI
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