Time-resolved fluorescence immunochromatography test strip, kit and preparation method for quantitative detection of human h-fabp

Abstract

The invention discloses a time-resolved fluorescent immunochromatography test paper strip for quantitatively detecting human H-FABP. The time-resolved fluorescent immunochromatography test paper strip comprises a bottom plate, a sample pad, a conjugating pad, a coating film and a water-absorbing paper, wherein the sample pad, the conjugating pad, the coating film and the water-absorbing paper are sequentially arranged on the bottom plate, the conjugating pad is coated with a fluorescent microsphere labeled H-FABP monoclonal detection antibody, the coating film comprises a detection zone and a control zone, the detection zone and the control zone are arranged in parallel at interval, the detection zone is coated with a H-FABP monoclonal capture antibody, the control zone is coated with a goat anti-mouse IgG antibody, and the H-FABP monoclonal detection antibody correspondingly has the antigenic epitope having the amino acid sequence represented by any one of SEQ ID No:1-SEQ ID No:4. According to the present invention, the individual quantitative detection of the H-FABP is achieved, the advantages of high sensitivity, stable marker, strong anti-interference and good repeatability of the detected result are provided, the operation is simple, the detection time is short, and the great convenience is provided for the clinical application.

Description

technical field [0001] The invention belongs to the technical field of immune detection, in particular, the invention relates to a time-resolved fluorescent immunochromatographic test strip, a test kit and a preparation method thereof for quantitatively detecting heart-type fatty acid binding protein (H-FABP). Background technique [0002] Heart-type fatty acid binding protein (H-FABP) is a new type of small molecular protein rich in the heart, which combines with two fatty acid molecules and participates in the metabolism of fat to provide energy for cardiomyocytes. It is highly specific for the heart. The concentration of H-FABP in blood is 2-10 times higher than that in skeletal muscle, and it can be quickly released into the blood when cardiomyocytes are damaged. As a marker, the sensitivity of diagnosing early AMI is 77.4%, and the specificity is 89.4%, and H-FABP The concentration can estimate the damaged area of ​​the myocardium. Therefore, H-FABP can be used as a re...

AI Technical Summary

Problems solved by technology

[0009] 2. Gold standard method - this method is fast, simple and easy to observe, but the sensitivity is not high

[0010] 3. Transmission immunoturbidimetry——This method is simple, fast, and can be automated, and is suitable for batch detection. However, the methodology and clinical application of immunoturbidimetry still need further verification

Various test strips and instruments based on immunochromatography, chromatography and dry chemistry techniques will affect the activity of microproteins in the matrix due to differences in temperature, humidity and pH, thereby affecting the results

The methodological flaws of some POCT instruments, such as poor sensitivity and repeatability, and narrow linear range, are only used for reference in emergencies or emergencies, and need to be sent to the laboratory for re-examination if necessary.

[0012] The current immunofluorescence chromatography device uses the reflection method to detect the fluorescent signal on the porous membrane. The fluorescence detector captures the specific antibody modified by the fluorescent dye on the surface of the porous membrane, but cannot detect the fluorescent signal inside the porous membrane, which leads to the detection of Sensitivity drop

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