Time-resolved fluorescent immunochromatographic test strip, kit and preparation method for detecting myo

Abstract

The invention discloses a time-resolved immunochromatographic test strip for detecting MYO, a kit and a preparation method thereof. The test strip comprises a bottom substrate, a sample pad, a binding pad, A coated membrane and absorbent paper, the binding pad is coated with MYO monoclonal detection antibody labeled with fluorescent microspheres, the coated membrane includes a detection area and a control area arranged in parallel and spaced apart from each other, and the detection area is coated with a recognition The MYO monoclonal capture antibody of a single epitope, the control area is coated with goat anti-mouse IgG antibody; the coating membrane is a nitrocellulose membrane combined with a polymer, and the polymer has a wavelength of less than 450nm with 10% The light transmittance below is a material that has a light transmittance of 95% or more at a wavelength of 500nm or more. The test strip of the invention can be used for rapid quantitative detection, the test result is accurate and reliable, the sensitivity is high, the preparation method is simple, the method is suitable for large-scale production, and it has positive significance for the quantitative detection of MYO.

Description

Technical field [0001] The present invention belongs to the technical field of medical testing. Specifically, the present invention relates to a time-resolved fluorescence immunochromatographic test strip for quantitative detection of MYO, a kit and a preparation method thereof. Background technique [0002] Myoglobin (Myoglobin, MYO) is a kind of oxygen-bound heme protein, mainly distributed in the myocardium and skeletal muscle tissue, accounting for about 0.1%-0.2% of the total muscle. The muscles of diving mammals such as whales, seals and dolphins are so rich in myoglobin that they make their muscles brownish red. Because myoglobin stores oxygen, these animals can be underwater for a long time. In acute myocardial injury, MYO is the first to be released into the blood. About 2-3 hours after the onset of symptoms, Mb in the blood can exceed the upper limit of normal, reaching a peak in 9-12 hours, and returning to normal after 24-36 hours. It can increase 1.5h after infarct...

AI Technical Summary

Problems solved by technology

[0007] 2. Gold standard method - this method is fast, simple and easy to observe, but the sensitivity of qualitative detection is not high

[0008] 3. Transmission immunoturbidimetry——This method is simple, fast, and can be automated, and is suitable for batch detection. However, the methodology and clinical application of immunoturbidimetry still need further verification

Various test strips and instruments based on immunochromatography, chromatography and dry chemistry techniques will affect the activity of microproteins in the matrix due to differences in temperature, humidity and pH, thereby affecting the results

The methodological flaws of some POCT instruments, such as poor sensitivity and repeatability, and narrow linear range, are only used for reference in emergencies or emergencies, and need to be sent to the laboratory for re-examination if necessary.

[0011] The current immunofluorescence chromatography device uses the reflection method to detect the fluorescent signal on the porous membrane. The fluorescence detector captures the specific antibody modified by the fluorescent dye on the surface of the porous membrane, but cannot detect the fluorescent signal inside the porous membrane, which leads to the detection of Sensitivity drop