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A kind of non-chemical solvent extraction method of Shenzimycin

A non-chemical technology, applied in the field of biological fermentation and purification, can solve problems such as difficulty in conforming to contemporary and future concepts, consumption of a large amount of organic solvents, high production costs, etc., to achieve advantages in production costs and product quality, and save energy. , safety and environmental protection effects

Active Publication Date: 2020-05-22
SHANGHAI NONGLE BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. The extraction workshop and extraction equipment must be fire and explosion-proof design, there are safety risks
[0005] 2. The extraction process consumes a large amount of organic solvents (ethyl acetate or chloroform, etc.), polluting the environment
[0006] 3. The process of concentration and crystallization requires heating and refrigeration systems, and the production cost is relatively high
[0008] The production of modern biopesticides attaches great importance to the concept of green, and the production process and products themselves must be safe and environmentally friendly. It is difficult to manufacture biopesticides according to the above-mentioned classic techniques in line with contemporary and future concepts

Method used

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  • A kind of non-chemical solvent extraction method of Shenzimycin
  • A kind of non-chemical solvent extraction method of Shenzimycin
  • A kind of non-chemical solvent extraction method of Shenzimycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Put 15 tons of Shenzimycin fermented liquid in the pretreatment tank, turn on the stirring, rotate at 30rpm, add 150L of 1.5% (w / v) composite biological flocculant sxn1 aqueous solution to the fermented liquid, continue to stir for 30 minutes, put in the tank The feed liquid gradually forms a large amount of granular suspension; filter the suspension feed liquid into the plate frame, collect the filtrate into the filtrate storage tank, wash the filter cake with tap water, and the top wash volume is 2 tons, and put it into the filtrate storage tank middle. Add 100L aqueous solution of composite biological flocculant sxn2 with a concentration of 1.0% (w / v) to the above-mentioned filtrate, stir for 20 minutes, and some flocs are formed again, and then filter the feed liquid into the plate frame to collect the filtrate In the filtrate storage tank, the filter cake is top-washed with tap water, and the top-wash volume is 2 tons, which is incorporated into the filtrate storag...

Embodiment 2

[0038]Put 15 tons of Shenzimycin fermented liquid in the pretreatment tank, start the stirring, the rotating speed is 30rpm, add 200L of composite biological flocculant sxn 1 aqueous solution with a concentration of 1.5% (w / v) to the fermented liquid, continue to stir for 30 minutes, tank The medium feed liquid gradually forms a large amount of granular suspension; the suspension feed liquid is filtered into the plate frame, the filtrate is collected into the filtrate storage tank, and the filter cake is top washed with tap water. in the can. Add 150L aqueous solution of composite biological flocculant sxn 2 with a concentration of 1.0% (w / v) to the above-mentioned filtrate, stir for 25 minutes, and some flocs are formed again, and then filter the feed liquid into a plate frame, collect and filter liquid into the filtrate storage tank, and the filter cake is top-washed with tap water, with a top-washing volume of 2 tons, and incorporated into the filtrate storage tank. The fi...

Embodiment 3

[0040] Put 15 tons of Shenzimycin fermented liquid in the pretreatment tank, turn on the stirring, rotate at 30rpm, add 250L of 1.5% (w / v) composite biological flocculant sxn1 aqueous solution to the fermented liquid, continue to stir for 30 minutes, put in the tank The feed liquid gradually forms a large amount of granular suspension; filter the suspension feed liquid into the plate frame, collect the filtrate into the filtrate storage tank, wash the filter cake with tap water, and the top wash volume is 2 tons, and put it into the filtrate storage tank middle. Add 200L aqueous solution of composite biological flocculant sxn2 with a concentration of 1.0% (w / v) to the above filtrate, stir for 25 minutes, and some flocs are formed again, then filter the feed liquid into the plate and frame, and collect the filtrate In the filtrate storage tank, the filter cake is top-washed with tap water, and the top-wash volume is 2 tons, which is incorporated into the filtrate storage tank. ...

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Abstract

The invention relates to a method for extracting phenazino-1-carboxylic acid by using a non-chemical solvent. The method comprises the following steps: adding a composite biological flocculating agent sxn1 to a fermentation solution to carry out primary flocculation, filtering and washing the obtained solution to obtain a primary filtrate, adding a composite biological flocculating agent sxn2 to the primary filtrate to carry out secondary flocculation, and filtering and washing the obtained solution to obtain a secondary filtrate; finely filtering the secondary filtrate to obtain a highly-pure fine filtrate, and adding an organic acid to the highly-pure fine filtrate to carry out crystallization; and centrifuging the crystallized solution, and drying obtained crystals to obtain the phenazino-1-carboxylic acid. A double-flocculation technology is adopted to remove polymeric mucopolysaccharides, proteins, thallus cells and other organic impurities generated in the fermentation process of M18 in order to highly purify the fermentation solution, and the fermented product is precipitated with the organic acid by using the negatively charged carboxylic group of the molecular structure of the phenazino-1-carboxylic acid in order to obtain the highly-pure solvent residual-free phenazino-1-carboxylic acid raw medicine.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation purification, and in particular relates to a method for extracting shenzamycin with a non-chemical solvent. Background technique [0002] Shenzimycin has another name called phenazine-1-carboxylic acid (phenazine-1-carboxylic acid, PCA), which is preserved by Pseudomonas fluorescens M18 (General Microorganism Center of China Microbiological Culture Collection Management Committee, preserved No.: CGMCC.NO.0462, which has been disclosed in Chinese patent CN1141379C) is a secondary metabolite produced by aerobic fermentation, which belongs to phenazine compounds, and has the molecular formula C 13 h 8 N 2 o 2 , Molecular weight 224, the crystalline pure product in organic solvent is yellow needle, melting point 241-242 ℃, soluble in organic solvents such as alcohol, ether, ethyl acetate, chloroform and benzene, slightly soluble in water, in acidic and neutral Stable under sexual c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D241/46
CPCC07D241/46
Inventor 张进武丁千岭张红艳龚新进冯镇泰
Owner SHANGHAI NONGLE BIOLOGICAL PROD