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Method for extracting and analyzing beta-agonists clenbuterol by adopting DPX (dispersive pipette extraction) tip type dispersive solid-phase microextraction column

A solid-phase microextraction column and receptor agonist technology, which is applied in the field of quantitative detection, can solve the problems of incompatibility with automated systems, a large number of samples and organic solvents, complicated operation processes, etc., and achieves improved detection sensitivity, small reagent consumption, Simple to use effects

Active Publication Date: 2017-12-29
无锡微色谱生物科技有限公司
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  • Summary
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  • Application Information

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Problems solved by technology

These methods also have various deficiencies, such as the need for a large amount of samples and organic solvents, complex and time-consuming operations, incompatibility with automated systems, etc.

Method used

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  • Method for extracting and analyzing beta-agonists clenbuterol by adopting DPX (dispersive pipette extraction) tip type dispersive solid-phase microextraction column
  • Method for extracting and analyzing beta-agonists clenbuterol by adopting DPX (dispersive pipette extraction) tip type dispersive solid-phase microextraction column
  • Method for extracting and analyzing beta-agonists clenbuterol by adopting DPX (dispersive pipette extraction) tip type dispersive solid-phase microextraction column

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specific Embodiment approach 1

[0043] Specific embodiment one: a kind of sample pretreatment method that utilizes DPX gun-tip type dispersive solid-phase microextraction column of the present embodiment to detect clenbuterol content rapidly, it comprises the following steps:

[0044] 1. Take the sample to be tested, add water or buffer, vortex and incubate overnight at 4°C;

[0045] 2. Add acetonitrile after homogenization, centrifuge after vortexing, and collect the supernatant after layering; evaporate the supernatant to dryness, dissolve it in water to obtain the pretreated sample;

[0046] 3. The above-mentioned pretreated sample is extracted through a solid-phase microextraction column, and rinsed with methanol and water in sequence;

[0047] 4. Put the pretreated sample on the column through the process of suction and beating;

[0048] 5. Wash the micro-extraction column with water, 1-3% formic acid / water solution by volume and 100% methanol by volume in sequence;

[0049] 6. The prepared mass and v...

specific Embodiment approach 2

[0051] Embodiment 2: This embodiment differs from Embodiment 1 in that: Step 5 washes the micro-extraction column with water, 2% formic acid / water solution by volume and 100% methanol by volume. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0052] Specific embodiment three: the difference between this embodiment and specific embodiment one is that the mass volume percentage content prepared in step 6 is 5% NH 4 OH / methanol solution to obtain sample eluate. Others are the same as in the first embodiment.

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Abstract

The invention provides a method for extracting and analyzing beta-agonists clenbuterol by adopting a DPX (dispersive pipette extraction) tip type dispersive solid-phase microextraction column and relates to the DPX tip type dispersive solid-phase microextraction column (DPX tips <TM>). The method can be matched with various manual and automatic pipetting systems, meat sample treatment and extraction can be completed rapidly and efficiently, and defects that traditional sample treatment is time-consuming and labor-consuming, instrument loss is increased and the like can be perfectly overcome. The simple and fast solid-phase extraction method is combined with HPLC-MS (high performance liquid chromatography-mass spectrometer) analysis, so that purification of 96-384 samples can be finished simultaneously within 10 min, and rapid, qualitative and quantitative high-flux determination for multiple kinds of beta-agonists clenbuterol in animal derived food can be realized.

Description

technical field [0001] The invention belongs to the field of food additive detection, and in particular relates to a method for extracting clenbuterol in animal source food by using a DPX gun-tip type dispersive solid-phase microextraction column, and an applicable quantitative detection method. Background technique [0002] my country is a big country in the pig industry, with about 640 million pigs slaughtered every year, and the total pork output ranks first in the world. However, due to the overall low level of pig breeding in my country and the imperfect management of the production and use of breeding medicines, the problem of illegal use of medicines in the breeding process is still prominent, especially the illegal use of "clenbuterol" has caused a huge negative impact at home and abroad. The resulting food safety issues have also aroused great concern at home and abroad. [0003] "Clenbuterol" strictly refers to a class of β-receptor agonist drugs. β-agonists are a...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 卡莫莱凯莉马赛妮比尔布鲁尔王倩王珏
Owner 无锡微色谱生物科技有限公司
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