Microcarriers, matrices and scaffolds for culturing mammalian cells and methods for their manufacture

Abstract

Disclosed herein are microcarriers, matrices and scaffolds for growing mammalian cells, including copolymer particles and matrices composed of polysaccharide-polyamine copolymers. The copolymer particles and matrix have a pore size of at least 50 microns and allow the growth of mammalian cells both on the outer surfaces of the particles and matrix and within the particles and matrix. Also disclosed herein are methods of making such microcarriers, matrices, and scaffolds, as well as combinations. In addition, provided herein are methods of growing mammalian cells using such microcarriers, matrices and scaffolds and combinations.

Description

[0001] This application claims the benefit of US Provisional Application No. 62 / 136,241, filed March 20, 2015, which is hereby incorporated by reference in its entirety. technical field

[0002] In general, the present disclosure relates to materials for culturing anchorage-dependent mammalian cells, and in particular to vectors for high-density growth of anchorage-dependent mammalian cells, Substrates and scaffolds, and methods for their manufacture. Background technique

[0003] The culture of mammalian cells, especially human cells, is critical in life sciences, pharmaceutical and biotechnology research, as cultures of human cells can be used to determine cell functions and interactions and, for example, to manufacture therapeutic cells, large quantities of Proteins and pathogens for vaccine development. Human gene products are commonly produced using in vitro methods via bacterial, yeast and insect gene expression systems. However, the normal biological function, activ...

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