Use of cxcl13 as a biomarker in diagnostic reagents

A technology of biomarkers and diagnostic reagents, which is applied in the field of preparation or screening of diagnostic reagents for Candida infection, and can solve problems such as the inapplicability of diagnostic methods to clinical applications

Active Publication Date: 2021-04-20
CHONGQING MEDICAL UNIVERSITY
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Problems solved by technology

[0004] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide the use of CXCL13 as a biomarker in the preparation or screening of diagnostic reagents for Candida infection, which is used to solve the diagnostic methods for invasive Candida infection in the prior art Not suitable for problems such as clinical application

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  • Use of cxcl13 as a biomarker in diagnostic reagents
  • Use of cxcl13 as a biomarker in diagnostic reagents
  • Use of cxcl13 as a biomarker in diagnostic reagents

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Experimental program
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Embodiment 1

[0034] Detection of Serum CXCL13 in Patients with Bloodstream Infection

[0035]The blood samples of clinical patients are tested for relevant pathogens by the microbiology room of the laboratory department. After the test is completed, the serum samples of the patients on the day of submission are collected. At the same time, the serum samples of patients with negative pathogen tests are collected as controls, and they are immediately stored in a -80°C refrigerator. For subsequent experiments. All patients were divided into 3 groups: candidemia group (candidemia group), bacteremia group (bacteremia group) and pathogen detection negative group (Control group), ELISA method was used to detect 56 candidemia patients, 56 bacterial The sera of patients with hyperemia and 36 patients with negative pathogen tests were collected. In addition, patients with cancer, autoimmune diseases and other acute infections that affect the expression of CXCL13 were excluded. The human CXCL13 ELIS...

Embodiment 2

[0038] ROC curve analysis compared the diagnostic performance of serum CXCL13, BDG, CRP and PCT in invasive candida infection.

[0039] The laboratory indicators of the candidemia group and the control group were collected through the laboratory LIS system, including G test (BDG), CRP and PCT. Use SPSS19.0 software to make ROC curve, and use MedCalc15.8 software to compare the statistical difference of the area under the ROC curve.

[0040] The result is as figure 2 As shown, the ROC curve analyzes the diagnostic value of different indicators in invasive candida infection, and the areas under the curve of serum CXCL13, CRP, PCT, and BDG are 0.917, 0.792, 0.769, and 0.934, respectively. CXCL13 vs CRP, P0.05.

[0041] From the statistical results, it was found that the area under the curve of CXCL13 was significantly larger than that of CRP and PCT, and it was statistically significant, indicating that CXCL13 had a certain diagnostic value for invasive candida infection. Alt...

Embodiment 3

[0043] In order to further explore the role of CXCL13 in invasive Candida infection, the present invention constructs a mouse model of Candida bloodstream infection. The experimental mice included 24 6-8 week old female C57BL / 6 mice (purchased from the Experimental Animal Center of Chongqing Medical University), were randomly divided into 4 groups, and were injected with different doses of Candida SC5314 through the tail vein, including high dose ( 3*10 5 CFU / piece), medium dose (1*10 5 CFU / piece), low dose (5*10 4 CFU / only), with sterile PBS as control, the experiment was repeated twice. The successful establishment of the mouse bloodstream infection model was verified by detecting the changes in the body weight of the mice and the number of Candida colonies in the kidneys. Results The normality analysis was carried out with SPSS19.0 software, and the graph was drawn with GraphPad Prism 5.0 software. Normality data with mean ± standard deviation , the results are shown ...

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Abstract

The present invention provides the use of CXCL13 as a biomarker in the preparation or screening of diagnostic reagents for Candida infection. The present invention finds that CXCL13 can be used as a new biomarker for diagnosing invasive Candida infection. Through in vivo animal experiments, different bacterial strains are found. Candida can cause an increase in the expression level of CXCL13, and the high expression of CXCL13 is positively correlated with the severity of the disease. Therefore, the present invention provides CXCL13 as a diagnostic and prognostic indicator for invasive Candida infection.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the use of CXCL13 as a biomarker in the preparation or screening of diagnostic reagents for Candida infection. Background technique [0002] Candida is one of the most common normal flora in the human intestinal tract, widely present in the external environment and in the human body. In recent decades, the incidence of Candida bloodstream infection has been increasing. The main risk factors include surgery, the use of invasive medical equipment, the use of broad-spectrum antibiotics, and some underlying diseases (diabetes, tumors, blood diseases, and chronic renal failure. Wait). According to reports, Candida ranks third or fourth in bloodstream infections caused by pathogens, among which Candida albicans is the most common pathogen of invasive fungal infections, followed by Candida parapsilosis and Candida glabrata. Candidemia has a high morbidity, mortality and a large amount of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6883C12Q1/04
Inventor 张莉萍曹炬李从雅
Owner CHONGQING MEDICAL UNIVERSITY
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