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A kind of bivalent inactivated vaccine of bovine pasteurellosis multocida and preparation method thereof

A technology of bivalent inactivated vaccine and Pasteurella, which can be used in antibacterial drugs, blood diseases, and pharmaceutical formulations, and can solve the problem of poor cross-immunity and the inability to effectively prevent and control bovine fibrinous suppurative pneumonia and bovine multocida Pasteurellosis is difficult to prevent and control, to achieve the effect of reducing side effects, good immune protection effect, and reducing the content of bacterial exotoxins

Active Publication Date: 2022-02-15
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of the poor cross immunity between different serotypes of Pasteurella multocida, the existing commercial products cannot effectively prevent and control the bovine fibrinous suppurative pneumonia caused by capsular type A bacteria, and give cattle Pasteurella multocida disease The prevention and control of the

Method used

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  • A kind of bivalent inactivated vaccine of bovine pasteurellosis multocida and preparation method thereof
  • A kind of bivalent inactivated vaccine of bovine pasteurellosis multocida and preparation method thereof
  • A kind of bivalent inactivated vaccine of bovine pasteurellosis multocida and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of Bivalent Inactivated Vaccine for Bovine Pasteurella Multocida

[0031] 1. Medium

[0032] Preparation of Brain Heart Infusion Medium (BHI): Weigh 37g of BHI powder and add it to 1000ml of distilled water, mix thoroughly, and then sterilize at 121°C for 15 minutes.

[0033] Preparation of Martin Broth Medium: Weigh 50g of Martin Broth Medium Powder and add it to 1000ml of distilled water. After mixing thoroughly, sterilize at 121°C for 15 minutes. After sterilization, add 0.1% lysed hemocyte whole blood according to the volume of the medium.

[0034] Brain Heart Infusion Agar Medium Preparation: Weigh 37g of BHI powder and 15g of agar powder into 1000ml of distilled water, mix thoroughly, sterilize at 121°C for 15 minutes, cool to 50°C to 60°C, pour the plates for later use.

[0035] Preparation of Martin Broth Agar Medium: Weigh 50g of Martin Broth Medium Powder and 15g of Agar Powder into 1000ml of distilled water, mix well, sterilize at 121°C...

Embodiment 2

[0055] Example 2 The safety test of the vaccine prepared by the present invention

[0056] 1 material

[0057] The vaccine that the method for 1.1 embodiment 1 makes bovine pasteurellosis multocida bivalent inactivated vaccine (A type Pm-TJ strain+B type C45-2 strain). The batch numbers are ZM201401, ZM201402, ZM201403 respectively.

[0058] 1.2 Experimental animals Healthy susceptible cattle, 3 months old and above.

[0059] 2 methods

[0060] 2.1 Safety test of a single-dose inoculation of target animals

[0061] 12 test cows were randomly divided into four groups, and 3 batches of vaccines were inoculated through the neck muscles with a single dose of 2.0ml (1 head), and 3 test cows with the same conditions were set up. Cattle were inoculated with sterilized PBS as a control in the same way. The clinical manifestations of the test cattle were observed, and the body temperature was measured at the same time every day for 14 days.

[0062] 2.2 Safety test of single-dose...

Embodiment 3

[0082] Example 3 Efficacy Test of the Vaccine Prepared by the Present Invention

[0083] 1 material

[0084] 1.1 The vaccine made by the method of Example 1 Bovine Pasteurella multocida bivalent inactivated vaccine (A type Pm-TJ strain+B type C45-2 strain). The batch numbers are ZM201401, ZM201402, ZM201403 respectively. The batch number of inactivated bovine pasteurellosis multocida vaccine (type B strain single vaccine, C45-2 strain, C46-2 strain, and C47-2 strain all contains) is 1407001.

[0085] 1.2 The experimental animals are healthy and susceptible cattle, aged 6 months and above.

[0086] 2 methods

[0087] 3 batches of vaccines prepared by the present invention were inoculated into 8 test cows with 1 part (2.0ml) dose each muscle, and the commercially available bovine pasteurellosis multocida inactivated vaccine (type B bacteria single vaccine) was injected with 1 cow. Each dose (4.0ml) was intramuscularly inoculated to 4 test cows, and another 6 non-immunized co...

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Abstract

The invention relates to a bivalent inactivated vaccine for bovine Pasteurella multocida and belongs to the technical field of preparation of veterinary biological products. The bivalent inactivated vaccine of the present invention is composed of an antigen and a vaccine adjuvant, wherein the antigen is the Pasteurella multocida capsular type A Pm-TJ strain and the Pasteurella multocida capsular type B C45-2 strain. The bivalent inactivated vaccine prepared by the present invention adopts a concentration and purification process, and optimizes the fermentation and cultivation process of the Pasteurella multocida capsular B type C45-2 strain, which shortens the fermentation time by more than half and improves production efficiency. The invented bivalent inactivated vaccine can simultaneously prevent bovine fibrinous suppurative pneumonia and bovine hemorrhagic sepsis caused by Pasteurella multocida infection through one-shot immunization, and has the characteristics of safety, reliability and good immune effect.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a bivalent inactivated vaccine against pasteurellosis multocida. Background technique [0002] Bovine Pasteurella multocida is an infectious disease caused by Pasteurella multocida infection in cattle. At present, there are mainly two types of Pasteurella multocida prevailing in my country, one is bovine fibrinous suppurative pneumonia caused by Pasteurella multocida capsule type A bacteria, and the other is caused by Pasteurella multocida capsule type A bacteria, and the other is caused by Pasteurella multocida capsule type A bacteria, Hemorrhagic septicemia in cattle caused by Pasteurella spp. capsulatus type B. Among them, bovine fibrinous suppurative pneumonia is a disease that seriously infringes on the respiratory system of cattle. Under the stimulation of stress conditions such as long-distance transportation after healthy cattle are mixe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/116A61K39/102A61P31/04A61P7/04A61P11/00
Inventor 徐和敏姜志刚邵葳于力潘春刚张爱民罗显绎姜晓红马爱荣周建民
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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