Preparation method and application of hybrid peptide with high arginine content in cervical cancer treatment

A high-arginine and mixed peptide technology is applied in the field of preparation of high-arginine mixed peptides to achieve the effects of delaying deterioration, inhibiting the proliferation of cancer cells and improving the quality of life

Active Publication Date: 2018-01-12
陈栋梁 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Preparation method and application of hybrid peptide with high arginine content in cervical cancer treatment
  • Preparation method and application of hybrid peptide with high arginine content in cervical cancer treatment
  • Preparation method and application of hybrid peptide with high arginine content in cervical cancer treatment

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Embodiment 1

[0034] The preparation method of homoarginine mixed peptide of the present invention comprises the following steps:

[0035] 1. Mix the degreased and crushed walnut meal with egg white protein at a mass ratio of 4:1, mix the protein meal mixture with water at a mass-volume ratio of 1:8, stir at room temperature for 2 hours, and then place it in an ultra-high pressure host system , the treatment pressure is 400MPa, and the holding time is 20min to obtain a suspension pretreated by ultra-high pressure; control the temperature of the suspension to 50°C, adjust the pH to 9, add alkaline protease with a mass of 3.5% of the suspension and stir evenly , at the same time, ultrasonic-microwave assisted enzymolysis, ultrasonic power 300W, ultrasonic time 12min, microwave power 400W, microwave time 8min, after 2h of enzymolysis, adjust the pH to 7, add papain with a mass of 3.5% of the suspension and stir evenly, while ultrasonic -Microwave-assisted enzymatic hydrolysis, ultrasonic power...

Embodiment 2

[0048] The preparation method of the mixed peptide of the present invention comprises the following steps:

[0049] 1. Mix the degreased and crushed walnut meal with egg white protein at a mass ratio of 3:1, mix the protein meal mixture with water at a mass-volume ratio of 1:6, stir at room temperature for 1.5 hours, and then place it in the ultra-high pressure host system In the middle, the treatment pressure is 300MPa, and the holding time is 15min to obtain a suspension pretreated by ultra-high pressure; control the temperature of the suspension to 50°C, adjust the pH to 9, add 3% alkaline protease of the suspension mass and stir Uniform, simultaneous ultrasonic-microwave assisted enzymolysis, ultrasonic power 200W, ultrasonic time 15min, microwave power 300W, microwave time 10min, after 1.5h of enzymolysis, adjust the pH to 7, add papain with 3% mass of the suspension and stir evenly, Simultaneously, ultrasonic-microwave assisted enzymolysis, ultrasonic power 200W, ultraso...

Embodiment 3

[0054] The preparation method of the mixed peptide of the present invention comprises the following steps:

[0055]1. Mix the degreased and crushed walnut meal particles with egg white protein at a mass ratio of 5:1, mix the protein meal mixture with water at a mass-volume ratio of 1:10, stir at room temperature for 2.5 hours, and place it in an ultra-high pressure host In the system, the processing pressure is 500MPa, and the pressure holding time is 25min to obtain a suspension pretreated by ultra-high pressure; the temperature of the suspension is controlled to 50°C, the pH is adjusted to 9, and 4% of the mass of the suspension is added to alkaline protease and Stir evenly, and at the same time, ultrasonic-microwave assisted enzymolysis, ultrasonic power 400W, ultrasonic time 20min, microwave power 500W, microwave time 5min, after 2.5h of enzymolysis, adjust the pH to 7, add papain with 4% mass of the suspension and stir well At the same time, ultrasonic-microwave assisted ...

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Abstract

The invention relates to a hybrid peptide with a high arginine content and an application thereof in cervical cancer treatment. The preparation method comprises the following steps: using an ultrahighpressure to preprocess walnut meal and egg-white protein suspension; using alkaline protease and papain to enzymatically hydrolyze the preprocessing product in steps under the assistance of ultrasonic waves and microwaves; filtering the enzymatic hydrolysate, subjecting obtained supernate to reversed phase high performance liquid chromatography so as to separate target hybrid peptides in the supernate; taking the hybrid peptides as the template molecule, taking acrylic acid and methacrylic acid as the functional monomers, taking triethylene glycol dimethacrylate as the crosslinking agent, taking isopropyl thioxanthone as the initiator, and carrying out polymerization triggered by UV rays to form a surface molecularly imprinted membrane, which is used to separate and enrich target hybrid peptides in the supernate of the enzymatic hydrolysate. The arginine content of obtained hybrid peptide is more than 18%. The MTT method detection result show that the hybrid peptide with a high arginine content can effectively inhibit the proliferation of human cervical cancer Hela cell. The provided method reduces the product cost and the industrial production is promoted.

Description

technical field [0001] The present invention relates to a preparation method of a high-arginine mixed peptide and its application in the treatment of cervical cancer, in particular to a method for preparing arginine-rich mixed peptide from walnut meal and egg white protein and its application in Application in the treatment of cervical cancer. Background technique [0002] Cancer is a general term for malignant tumors, characterized by the loss of normal regulatory control of body cells, excessive proliferation, and different degrees of differentiation disorders, and often invades adjacent tissues or metastasizes to distant sites. It seriously threatens the health of all human beings and ranks first among the causes of abnormal death. According to the "Global Cancer Report" released by the World Health Organization (WHO) in 2014, the number of new cancer cases in China ranked first in the world in 2012. In 2015, the "Three-Year Action Plan for Cancer Prevention and Control...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/20C07K1/14A61K38/01A61P35/00A23L33/18
CPCA23L33/18C07K1/22A61K36/52A61K38/168A61K38/1709B01D15/325B01D15/3852B01D15/362A61K38/00A61K2236/15A61K2236/13A61K2236/39A61K2236/53A61P35/00C07K1/36C07K1/34C07K1/145C07K1/042C07K1/18A61K35/57A23L33/175A23V2002/00
Inventor 陈栋梁张阳刘立坚何立蓉
Owner 陈栋梁
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