Detection reagent for detecting mercapto compounds in urine as well as preparation method and application of detection reagent

Abstract

The invention discloses a detection reagent for detecting mercapto compounds in urine. The detection reagent comprises a testing reagent, a control reagent and a color-developing reagent according toa volume ratio of 1:1:2, wherein the testing reagent is purified water, the control reagent is an aqueous solution of mercury-containing compounds and cadmium-containing compounds, and the color-developing reagent is a color-developing buffer solution containing phosphotungstic acid, phosphomolybdic acid and a lithium-containing compound. A preparation method comprises the following steps: (1), performing split charging on the testing reagent; (2), preparing the control reagent: dissolving the mercury-containing compounds and the cadmium-containing compounds into the purified water; (3), preparing the color-developing reagent: mixing a mixed aqueous solution of the phosphotungstic acid, the phosphomolybdic acid and the lithium-containing compound with a buffer solution. The invention has the advantages as follows: interference of uric acid, Vc and other reducing substances on mercapto groups is effectively avoided, so that occurrence of a false negative result is avoided, and thus a detection result is more accurate and reliable; in addition, the preparation method is simple and convenient, the cost is low, and large-scale production is easy.

Description

technical field [0001] The invention belongs to the technical field of biochemical detection, and specifically relates to a detection reagent for mercapto compounds in urine, a preparation method and application thereof. Background technique [0002] Among the malignant tumors that endanger women's health in the world, cervical cancer is listed third. According to statistics, in 2008, among all new cancers in women worldwide, the proportion of new cervical cancer was 9% (529,800 cases). ), second only to breast cancer and colorectal cancer. Among the patients who died of cancer among women, 8% (275,100 cases) of female cancer patients died of cervical cancer, ranking fourth. Among the patients who died of cervical cancer, about 230,000 people belonged to developing countries, accounting for about 83% of the total number. In my country, according to statistics, there are about 130,000 new cases of cervical cancer every year. [0003] Cervical cancer is one of the most comm...

AI Technical Summary

Problems solved by technology

[0005] However, the composition of human urine is complex, and the urine composition varies greatly among different people. In particular, certain trace components in urine or abnormal metabolic products caused by other causes will also combine with mercury ions in the detection reagent to form Color precipitation will seriously interfere with the accuracy of the test results. Therefore, although the current qualitative detection reagents for thiol compounds are highly sensitive, they often have false negative problems.