Detection reagent for detecting mercapto compounds in urine as well as preparation method and application of detection reagent

A technology of mercapto compounds and detection reagents, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions. It can solve problems such as complex components, interference with the accuracy of test results, and false negatives. , to achieve the effect of simple preparation method, convenient observation and judgment, and easy operation

Inactive Publication Date: 2018-01-12
SHENZHEN HUACHUANG BIO PHARM TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the composition of human urine is complex, and the urine composition varies greatly among different people. In particular, certain trace components in urine or abnormal metabolic products caused by other causes will also combine with mercury ions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the detection reagent of mercapto compound in the urine provided by the present invention, comprises the test reagent, contrast reagent, chromogenic reagent that volume ratio is 1:1:2, and test reagent is purified water, and contrast reagent is mercury-containing compound, An aqueous solution containing cadmium compounds, and the color developing reagent is a color developing buffer solution containing phosphotungstic acid, phosphomolybdic acid and lithium compounds.

[0031] The mercury-containing compound and the cadmium-containing compound specifically selected are mercuric chloride and cadmium chloride respectively, and their concentrations in the contrast reagent are respectively 0.04 mol / L of mercuric chloride and 0.001 mol / L of cadmium chloride.

[0032] The selected color reagent is specifically a mixed solution containing phosphotungstic acid, phosphomolybdic acid, and an aqueous solution containing lithium compounds mixed with a buffer solution at...

Embodiment 2

[0049] Embodiment 2: the detection reagent of mercapto compound in the urine provided by the present invention, comprises the test reagent, contrast reagent, color developing reagent that volume ratio is 1: 1: 2, and test reagent is purified water, and contrast reagent is mercury-containing compound, An aqueous solution containing cadmium compounds, and the color developing reagent is a color developing buffer solution containing phosphotungstic acid, phosphomolybdic acid and lithium compounds.

[0050] The specifically selected mercury-containing compound and cadmium-containing compound are respectively mercury sulfate and cadmium sulfate, and their concentrations in the contrast reagent are respectively 0.08 mol / L of mercury sulfate and 0.001 mol / L of cadmium sulfate.

[0051] The selected color reagent is specifically a mixed solution containing phosphotungstic acid, phosphomolybdic acid, and an aqueous solution containing lithium compounds mixed with a buffer solution at a ...

Embodiment 3

[0065] Embodiment three: the detection reagent of mercapto compound in the urine that the present invention provides, comprises the test reagent, contrast reagent, color development reagent that volume ratio is 1: 1: 2, and test reagent is purified water, and contrast reagent is mercury-containing compound, An aqueous solution containing cadmium compounds, and the color developing reagent is a color developing buffer solution containing phosphotungstic acid, phosphomolybdic acid and lithium compounds.

[0066] The specifically selected mercury-containing compound and cadmium-containing compound are respectively mercury nitrate and cadmium nitrate, and their concentrations in the contrast reagent are respectively 0.06 mol / L of mercury nitrate and 0.001 mol / L of cadmium nitrate.

[0067] The selected color reagent is specifically a mixed solution containing phosphotungstic acid, phosphomolybdic acid, and an aqueous solution containing lithium compounds mixed with a buffer solutio...

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PUM

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Abstract

The invention discloses a detection reagent for detecting mercapto compounds in urine. The detection reagent comprises a testing reagent, a control reagent and a color-developing reagent according toa volume ratio of 1:1:2, wherein the testing reagent is purified water, the control reagent is an aqueous solution of mercury-containing compounds and cadmium-containing compounds, and the color-developing reagent is a color-developing buffer solution containing phosphotungstic acid, phosphomolybdic acid and a lithium-containing compound. A preparation method comprises the following steps: (1), performing split charging on the testing reagent; (2), preparing the control reagent: dissolving the mercury-containing compounds and the cadmium-containing compounds into the purified water; (3), preparing the color-developing reagent: mixing a mixed aqueous solution of the phosphotungstic acid, the phosphomolybdic acid and the lithium-containing compound with a buffer solution. The invention has the advantages as follows: interference of uric acid, Vc and other reducing substances on mercapto groups is effectively avoided, so that occurrence of a false negative result is avoided, and thus a detection result is more accurate and reliable; in addition, the preparation method is simple and convenient, the cost is low, and large-scale production is easy.

Description

technical field [0001] The invention belongs to the technical field of biochemical detection, and specifically relates to a detection reagent for mercapto compounds in urine, a preparation method and application thereof. Background technique [0002] Among the malignant tumors that endanger women's health in the world, cervical cancer is listed third. According to statistics, in 2008, among all new cancers in women worldwide, the proportion of new cervical cancer was 9% (529,800 cases). ), second only to breast cancer and colorectal cancer. Among the patients who died of cancer among women, 8% (275,100 cases) of female cancer patients died of cervical cancer, ranking fourth. Among the patients who died of cervical cancer, about 230,000 people belonged to developing countries, accounting for about 83% of the total number. In my country, according to statistics, there are about 130,000 new cases of cervical cancer every year. [0003] Cervical cancer is one of the most comm...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 刘东
Owner SHENZHEN HUACHUANG BIO PHARM TECH CO LTD
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