Sand bed seedling culture method for Artocarpus nanchuanensis S. S.
A sand bed and seed technology, which is applied in the field of plant cultivation, can solve the problems of restricting the rescue and protection work of Nanchuan Boluo and the low emergence rate of sowing, and achieve the effects of avoiding seedling infection by germs, low cost, and increasing the number of lateral roots
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preparation Embodiment 1
[0032] According to the ratio of chitosan quality: copper sulfate volume is 1 / 4, chitosan is added to the copper sulfate solution with an initial concentration of 15g / L, sulfuric acid is added dropwise to adjust the pH to 4-5, at 40°C, 120W ultrasonic, Oscillate under the condition of 50kHz oscillation frequency for 90min, filter and dry the filter residue with suction to obtain copper-loaded chitosan; add 10 times the volume mass fraction of the obtained copper-loaded chitosan to 2% acetic acid aqueous solution, mechanically stir until dissolved, and then Add cellulose powder with 1 times the weight of chitosan, and stir at room temperature for 2 hours to obtain a mixed solution; cast the obtained mixed solution on a glass substrate, and then dry it at 70°C, then use 2mol / L ammonia solution Soak for 10 minutes, then wash with deionization several times, and dry in vacuum at 65°C, 0.09MPa~0.1MPa for 2 hours to obtain an antibacterial film.
preparation Embodiment 2
[0034]According to the ratio of chitosan quality: copper sulfate volume is 1 / 4, chitosan is added to the copper sulfate solution with an initial concentration of 20g / L, sulfuric acid is added dropwise to adjust the pH to 4-5, at 40°C, 120W ultrasonic, Oscillate under the condition of 50kHz oscillation frequency for 90min, filter and dry the filter residue with suction to obtain copper-loaded chitosan; add 20 times of the obtained copper-loaded chitosan into 2% acetic acid aqueous solution, mechanically stir until dissolved, and then Add cellulose powder twice the weight of chitosan, stir at room temperature for 2 hours to obtain a mixed solution; tape-cast the obtained mixed solution on a glass substrate, dry it at 70°C, and wash it with 3mol / L ammonia solution Soak for 10 minutes, then wash with deionization several times, and dry in vacuum at 65°C, 0.09MPa~0.1MPa for 2 hours to obtain an antibacterial film.
preparation Embodiment 3
[0036] According to the ratio of chitosan quality: copper sulfate volume is 1 / 4, chitosan is added to the copper sulfate solution with an initial concentration of 17g / L, sulfuric acid is added dropwise to adjust the pH to 4-5, at 40°C, 120W ultrasonic wave, Oscillate under the condition of 50kHz oscillation frequency for 90min, filter and dry the filter residue with suction to obtain copper-loaded chitosan; add 15 times the volume mass fraction of the obtained copper-loaded chitosan to 2% acetic acid aqueous solution, mechanically stir until dissolved, and then Add cellulose powder 1.5 times the weight of chitosan, and stir at room temperature for 2 hours to obtain a mixed solution; cast the obtained mixed solution on a glass substrate, and then dry it at 70°C, and use 2.5mol / L ammonia Soak in aqueous solution for 10 minutes, then wash with deionization several times, and dry in vacuum at 65°C, 0.09MPa~0.1MPa for 2 hours, and then obtain an antibacterial film.
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