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Triploid rainbow trout fingerling production technology

A rainbow trout and triploid technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of low induction rate and high mortality rate, and achieve the effect of reducing mortality and ensuring induction rate

Inactive Publication Date: 2018-01-16
重庆市同盟农业开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the cultivation of triploid rainbow trout, the commonly used temperature shock method has a low induction rate and a high mortality rate.

Method used

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  • Triploid rainbow trout fingerling production technology

Examples

Experimental program
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Effect test

Embodiment 1 3

[0028] Example 1 Seed Production Techniques for Triploid Rainbow Trout

[0029] The seed production technique method comprises the following steps:

[0030] (1) Construction and management of fish ponds: Build a flowing fish pond with a water depth of 60 cm and a pool wall height of 90 cm. There is a certain slope from the water inlet at the bottom of the pond to the drainage point. The water velocity is 12-18 m / min, and the pH value of the water in the fish pond is 6.5-6.8.

[0031] (2) Selection of broodstock: Select rainbow trout male broodstock and rainbow trout tetraploid female broodstock with strong physique, no disease, and well-developed gonads for artificial induction;

[0032] (3) Injection of oxytocic agent: oxytocic agent was injected into the thoracic cavity of tetraploid female broodstock of rainbow trout. The oxytonic agent was selected from luteinizing hormone-releasing hormone analogs, dioconone and 0.7% normal saline for fish; the drug was injected twice, a...

Embodiment 2 3

[0037] Embodiment 2 Triploid rainbow trout seed production technology method

[0038] The seed production technique method comprises the following steps:

[0039] (1) Construction and management of fish ponds: Build a flowing fish pond with a water depth of 60 cm and a pool wall height of 90 cm. There is a certain slope from the water inlet at the bottom of the pond to the drainage point. The water velocity is 12-18 m / min, and the pH value of the water in the fish pond is 6.5-6.8.

[0040] (2) Selection of broodstock: Select rainbow trout male broodstock and rainbow trout tetraploid female broodstock with strong physique, no disease, and well-developed gonads for artificial induction;

[0041] (3) Injection of oxytocic agent: oxytocic agent was injected into the thoracic cavity of tetraploid female broodstock of rainbow trout. The oxytonic agent was selected from luteinizing hormone-releasing hormone analogs, dioconone and 0.7% normal saline for fish; the drug was injected tw...

Embodiment 3

[0046] Example 3 Seed Production Techniques for Triploid Rainbow Trout

[0047] The seed production technique method comprises the following steps:

[0048] (1) Construction and management of fish ponds: Build a flowing fish pond with a water depth of 60 cm and a pool wall height of 90 cm. There is a certain slope from the water inlet at the bottom of the pond to the drainage point. The water velocity is 12-18 m / min, and the pH value of the water in the fish pond is 6.5-6.8.

[0049] (2) Selection of broodstock: Select rainbow trout male broodstock and rainbow trout tetraploid female broodstock with strong physique, no disease, and well-developed gonads for artificial induction;

[0050] (3) Injection of oxytocic agent: oxytocic agent was injected into the thoracic cavity of tetraploid female broodstock of rainbow trout. The oxytonic agent was selected from luteinizing hormone-releasing hormone analogs, dioconone and 0.7% normal saline for fish; the drug was injected twice, a...

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Abstract

The invention discloses a triploid rainbow trout fingerling production technology. The fingerling production technology comprises the following steps: performing artificial insemination 24 hours afterinjecting an oxytocic, and collecting a fertilized ovum to perform heat shock and electric shock combined treatment; standing the fertilized ovum in cold running water at the temperature of 9-10 DEGC for 10-20 minutes; treating at the temperature of 18-20 DEG C for 10-12 minutes, and performing electric shock treatment for 15-20 minutes under 20-25 DEG C and alternating current of 5-8V so as toobtain a triploid rainbow trout fertilized ovum; putting the fertilized ovum into a hatching barrel, hatching by using running water at flow velocity of 0.4m / s of the fertilized ovum before deciduation and at flow velocity of 0.2m / s of the fertilized ovum after deciduation, and breeding triploid rainbow trout fingerlings. The fingerling production technology for performing two-step shock treatmentis researched in the invention. The heat treatment temperature is lower than that in the prior art, and electric shock is combined at the second stage. With the adoption of the method disclosed by the invention, a death rate caused by sudden temperature rise can be reduced, and an induction rate is ensured. The survival rate of the fertilized ovum of the rainbow trout reaches 92% or higher, and the triploid induction rate of the fertilized ovum is 80% or higher.

Description

technical field [0001] The invention relates to the technical field of fish reproduction, in particular to a seed production method for triploid rainbow trout. Background technique [0002] Rainbow trout is a cold-water pond fish of the family Salmonidae and the genus Pacific Salmon. Rainbow trout is an important cold-water fish widely farmed in the world. The mature individual has a brown-red longitudinal stripe along the lateral line, which looks like a rainbow, hence the name. It is native to the northern part of North America and the west coast of the Pacific Ocean. It mainly lives in low-temperature fresh water and has high water quality requirements for aquaculture waters. Steelhead trout is a food fish that has been imported into several countries but has become an invasive species in some areas. [0003] Triploid rainbow trout is an all-female rainbow trout produced by hybridization of tetraploid rainbow trout and common diploid rainbow trout. It has the characte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10
CPCY02A40/81
Inventor 魏成阳
Owner 重庆市同盟农业开发有限公司
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