Method for extracting total flavonoids from herba desmodii styracifolii

A technology for widening the bellflower and total flavonoids, applied in the field of extracting total flavonoids, can solve the problems of demanding equipment, cumbersome operation, oxidation and the like

Inactive Publication Date: 2018-01-16
桂林纽泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the common solvent C0 2 It has the characteristics of non-polarity and small relative molecular mass, which makes the extraction effect unsatisfactory due to its low solubility and low selectivity for some substances. In actual operation, it is often necessary to increase the extraction rate by adding entraining agents. Finally, it is necessary to try to remove the entraining agent in the volatile oil, which will inevitably lead to the complexity of the process and the loss and oxidation of volatile components; in addition, because water is insoluble in CO 2 , so supercritical CO 2 The extraction process often needs to pre-dry the raw materials, which increases the cost and causes the loss of aromatic compound oil
In addition, when extracting volatile oils from plants, due to supercritical CO 2 It has similar solubility not only to volatile oils but also to low-polarity compounds such as waxes, fatty acids, colored substances and resins in the stratum corneum. These substances will also be extracted during the extraction process, although complex systems can also be used. Pure volatile oil, but the operation is cumbersome and the requirements for equipment are more stringent

Method used

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  • Method for extracting total flavonoids from herba desmodii styracifolii

Examples

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Embodiment 1

[0023] A method for extracting total flavonoids from Desmodium glabra, specifically comprising the steps of:

[0024] 1) Clean Desmodium sativa, dry, pulverize with a pulverizer, and cross a 20-mesh sieve;

[0025] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 150-250°C, pressure 8-10Mpa, extraction time 0.5h, extract Desmodium sativa, after the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature to obtain the extract;

[0026] 3) Purification: Concentrate the extract obtained in step 2), control the temperature ≤ 50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, perform column chromatography through DM130 macroporous resin, and load ...

Embodiment 2

[0028] A method for extracting total flavonoids from Desmodium glabra, specifically comprising the steps of:

[0029] 1) Clean Desmodium sativa, dry, pulverize with a pulverizer, and pass through a 40-mesh sieve;

[0030] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 150-250°C, pressure 8-10Mpa, extraction time 1.0h, extract Desmodium sativa, after the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature to obtain the extract;

[0031] 3) Purification: Concentrate the extract obtained in step 2), control the temperature to ≤50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, and perform column chromatography through AB-8 macroporous resi...

Embodiment 3

[0033] A method for extracting total flavonoids from Desmodium glabra, specifically comprising the steps of:

[0034] 1) Clean Desmodium sativa, dry, pulverize with a pulverizer, and cross an 80-mesh sieve;

[0035]2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 150-250°C, pressure 8-10Mpa, extraction time 1.0h, extract Desmodium sativa, after the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature to obtain the extract;

[0036] 3) Purification: Concentrate the extract obtained in step 2), control the temperature ≤ 50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, perform column chromatography through D101 macroporous resin, and load t...

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Abstract

The invention discloses a method for extracting total flavonoids from herba desmodii styracifolii. The method comprises the following steps: (1) cleaning and airing the herba desmodii styracifolii, crushing by using a crushing machine, and screening; (2) performing subcritical water extraction, namely, placing the treated raw material in an extracting kettle, adding hydroxyethyl cellulose which isequivalent to 0.001 weight part of the raw material, injecting deoxygenated deionized water into the kettle, extracting the herba desmodii styracifolii, relieving pressure after the extracting is ended, enabling extracting liquid to flow into a collecting tank, and cooling to room temperature to obtain the extracting liquid, wherein the extracting temperature is 150 to 250 DEG C, the pressure is8 to 10 Mpa, and the extracting time is 0.5 to 1.0 h; (3) purifying, namely, concentrating the extracting liquid which is obtained in the step (2), performing column chromatography through macroporousresin, eluting by using 50 percent v / v ethyl alcohol, thus obtaining eluant, concentrating, and drying to obtain a finished product.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting total flavonoids from Desmodium sativa. Background technique [0002] Herba Desmodium styracifolii (Herba Desmodii styracifolii) is a commonly used Chinese herbal medicine. It is the aboveground dry part of Desmodium styracifolium (Osh.) Merr. in the leguminous plant, and it is included in the 2010 edition of Pharmacopoeia of the People's Republic of China. The original plant is a semi-shrub-like herb, distributed in Guangdong, Guangxi, Fujian, Hunan and other provinces and regions. Guangdong is the main production area of ​​medicinal materials, and its branches and leaves are mainly used as medicine. Road infection, urinary calculi, gallbladder stones, nephritis edema and other diseases. Many of the prescriptions used to treat urinary calculus diseases use Desmodium sativa as the monarch drug, and its curative effect is quite good. D...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61K47/38
Inventor 谢冬养
Owner 桂林纽泰生物科技有限公司
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