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A kind of yeast peptone and its preparation method and application

A technology of yeast peptone and peptone, which is applied in the field of yeast peptone and its preparation, can solve problems such as the need to improve nutritional value and low total nitrogen content, and achieve the effects of shortening fermentation time, reducing production cost, and improving production efficiency

Active Publication Date: 2021-03-26
ANGELYEAST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Yeast peptone is an organic nitrogen source first invented by Angel Yeast Co., Ltd. The process steps are: mixing fresh yeast milk, autolysis, enzymatic hydrolysis, centrifugation, membrane filtration enrichment, and concentrated drying; the yeast The molecular weight distribution of peptone is 500-3000Da, but the yeast peptone prepared by the previous process has low total nitrogen content and / or peptone content, and its nutritional value needs to be improved

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  • A kind of yeast peptone and its preparation method and application
  • A kind of yeast peptone and its preparation method and application
  • A kind of yeast peptone and its preparation method and application

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preparation example Construction

[0037] In order to solve the problem of low total nitrogen content and / or peptone content of common yeast peptone, the present invention provides a yeast peptone with high total nitrogen and high protein and its preparation method and application.

[0038] The present invention pre-treats the yeast slurry before autolysis and enzymolysis, and treating the yeast slurry under alkaline conditions can increase the permeability of the yeast cell wall, and does not add surfactants or perform other procedures before enzymolysis. Treatment, only treatment under alkaline conditions can save the process of removing surfactants and save treatment time.

[0039] The present invention adopts protease for treatment, and the enzyme concentration is 0.2%-0.8% (wt) of yeast dry matter.

[0040] Specifically, in a preferred embodiment, the preparation method of yeast peptone of the present invention includes the following scheme:

[0041] Fresh yeast milk→acid-base treatment→filtration→heavy p...

Embodiment 1

[0067] To prepare high-protein yeast peptone powder, the steps are as follows:

[0068] 1. Using molasses as a medium to specially cultivate Saccharomyces cerevisiae

[0069] (1) Preparation of Saccharomyces cerevisiae seed liquid

[0070] Weigh 5g of Saccharomyces cerevisiae, add 50mL of 2wt% glucose solution, and activate at 28°C for 30min to obtain Saccharomyces cerevisiae seed liquid.

[0071] (2) Preparation of yeast fermentation broth

[0072] With 30wt% molasses as a carbon source, 16wt% ammonia water, 10wt% ammonium dihydrogen phosphate and yeast extract powder as a mixed nitrogen source, add it to a 50L fermenter to form a 30L medium, wherein the ammonia water and phosphoric acid The volume ratio of ammonium dihydrogen is 2:1, the amount of yeast extract powder added is 2% of the total weight of the medium, and the carbon-nitrogen ratio in the final medium is 10:1. The pH was adjusted to 4.0 with sulfuric acid. Add 300 mL of Saccharomyces cerevisiae seed liquid to...

Embodiment 2

[0108] To prepare high-protein yeast peptone powder, the steps are as follows:

[0109] 1. Using molasses as a medium to specially cultivate Saccharomyces cerevisiae

[0110] (1) Preparation of Saccharomyces cerevisiae seed liquid

[0111] Weigh 5g of Saccharomyces cerevisiae, add 50mL of 2wt% glucose solution, and activate at 28°C for 30min to obtain Saccharomyces cerevisiae seed liquid.

[0112] (2) Preparation of yeast fermentation broth

[0113] With 35wt% molasses as a carbon source, 18wt% ammonia water, 15wt% ammonium dihydrogen phosphate and yeast extract powder as a mixed nitrogen source, add it to a 50L fermenter to form a 30L medium, wherein the ammonia water and phosphoric acid The volume ratio of ammonium dihydrogen is 4:1, the amount of yeast extract powder added is 5% of the total weight of the medium, and the carbon-nitrogen ratio in the final medium is 8:1. The pH was adjusted to 4.0 with sulfuric acid. Add 300 mL of Saccharomyces cerevisiae seed liquid to ...

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Abstract

The invention relates to the technical field of biological products, in particular to a yeast peptone and its preparation method and application. The total nitrogen content in the yeast peptone is 13-14.5%, the amino acid nitrogen content is 2.0-4.5%, the peptone content is 35-60%, and the molecular weight of the peptone is 500-3000D. The preparation method of yeast peptone includes: (1) pretreatment of yeast slurry: treating the yeast slurry under alkaline conditions; (2) autolysis and enzymolysis process: adding protease to the yeast slurry for autolysis and enzymolysis to obtain Enzymolysis solution; (3) Separating and obtaining yeast peptone. Applying the yeast peptone of the present invention to the field of biological fermentation can improve the production efficiency of the biological fermentation industry, shorten the fermentation time, increase the fermentation titer, and reduce the production cost of customers.

Description

technical field [0001] The invention relates to the technical field of biological products, in particular to the technical field of preparation of yeast peptone, in particular to a yeast peptone and its preparation method and application. Background technique [0002] Yeast peptone (yeast peptone) is a powdery, paste or Liquid product. It is rich in proteins, amino acids, peptides, nucleotides, B vitamins, and trace elements. Its main function is to provide nitrogen sources, various vitamins, amino acids, and growth factors required for microbial growth. It is widely used in the fermentation medium industry. , is a new, clean and efficient source of organic nitrogen. [0003] Yeast peptone is an organic nitrogen source first invented by Angel Yeast Co., Ltd. The process steps are: mixing fresh yeast milk, autolysis, enzymatic hydrolysis, centrifugation, membrane filtration enrichment, and concentrated drying; the yeast The molecular weight distribution of peptone is 500-3...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
Inventor 李啸俞学锋李知洪姚鹃杜支红伍业旭陈林
Owner ANGELYEAST CO LTD