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Camel blood polypeptide with anti-fatigue effect and preparation method thereof

The technology of camel blood and camel is applied in the field of camel blood polypeptide with anti-fatigue effect and its preparation, which can solve the problem of short supply of products and achieve the effects of good regeneration performance, high efficiency and promotion of industrial development.

Active Publication Date: 2021-05-07
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, with the improvement of people's living standards, the demand for functional peptide products from high-end consumer groups in large and medium-sized cities in China is still increasing, and the market for related products is still in short supply in the short term.

Method used

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  • Camel blood polypeptide with anti-fatigue effect and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] First put 100g of fresh camel blood in a fermenter, add 200g of pure water, stir evenly at a speed of 220 rpm, and pre-treat with high-temperature steam at a temperature of 112°C, control the system temperature at 45°C, and use HCl and NaOH to adjust the pH value to 5.5, then add 0.5% Protamex complex protease to the fermenter for enzyme digestion, after 2 hours of enzyme digestion, then raise the temperature of the fermenter to 85°C and keep it warm for 15 minutes to sterilize the enzyme.

[0021] Cool the enzyme-sterilized camel blood enzymatic hydrolyzate to room temperature, centrifuge at 8000 rpm, collect the centrifugate, and obtain camel blood polypeptide extract.

[0022] Weigh 100g XDA-8 type macroporous adsorption resin, 100g LX-68 type macroporous adsorption resin and 100g HPD750 type macroporous adsorption resin, mix them evenly in the aqueous solution, and wet them into a glass with an inner diameter of 4cm and a column height of 60cm In the column, use the...

Embodiment 2

[0031] First put 300g of frozen camel blood in a fermenter, add 1200g of pure water, stir evenly at a speed of 250 rpm, and pre-treat with high-temperature steam at a temperature of 115°C, control the system temperature at 50°C, and use HCl and NaOH to adjust the pH value to 6.0, then add 2% compound flavor protease to the fermenter for enzymatic digestion, after 5 hours of enzymatic digestion, then raise the temperature of the fermenter to 90°C and keep it warm for 20 minutes to sterilize the enzyme.

[0032] Cool the enzyme-sterilized camel blood enzymatic hydrolyzate to room temperature, centrifuge at 10,000 rpm, collect the centrifugate, and obtain camel blood polypeptide extract.

[0033] Weigh 300g XDA-8 type macroporous adsorption resin, 600g LX-68 type macroporous adsorption resin and 900g HPD750 type macroporous adsorption resin, mix them evenly in the aqueous solution, and wet them into a glass with an inner diameter of 8cm and a column height of 120cm In the column,...

Embodiment 3

[0036] First, put 1000g of fresh camel blood in a fermenter, add 6000g of pure water, stir evenly at a speed of 300 rpm, and pre-treat with high-temperature steam at a temperature of 120°C, control the temperature of the system at 55°C, and use HCl and NaOH to adjust the pH value to 6.5, then add 5% Protamex compound protease and compound flavor protease (1:3) to the fermenter for enzymatic digestion. Minutes for enzyme sterilization.

[0037] Cool the enzyme-sterilized camel blood enzymatic hydrolyzate to room temperature, centrifuge at high speed, collect the supernatant, and obtain the camel blood polypeptide extract; centrifuge at 18,000 rpm, collect the centrifugate, and obtain the camel blood polypeptide extract.

[0038] Weigh 400g XDA-8 type macroporous adsorption resin, 1200g LX-68 type macroporous adsorption resin and 800g HPD750 type macroporous adsorption resin, mix them evenly in the aqueous solution, and put them into a glass with an inner diameter of 12cm and a ...

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Abstract

The invention relates to a camel blood polypeptide with anti-fatigue effect and a preparation method thereof. The content of the camel blood polypeptide product prepared by the invention is more than 80%, and the recovery rate is more than 75%. Compared with the prior art method, the present invention is characterized in that it provides a preparation process of camel blood polypeptide with anti-fatigue activity; using high-temperature steam for pretreatment not only accelerates the temperature rise of the system, but also changes the protein, which is beneficial to enzyme The entire production process uses green solvents, which are low in cost, high in efficiency, and environmentally friendly; the process route is simple, and it can provide food, health care products, and medicines with anti-fatigue activity camel blood peptides.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of natural products, and in particular relates to a camel blood polypeptide with anti-fatigue effect and a preparation method thereof. Background technique [0002] Camels are animals of the genus Camelus (Camelus) in the family Camelidae. They are one of the important livestock resources in my country and have important economic value. The protein content of camel blood is 20.51%. Of the total protein content in camel serum, the albumin content is as high as 73.20%. The ratio of albumin to globulin is 2.72, which is significantly different from that of sheep, cattle, and horses. Because camels live in harsh and harsh environmental conditions, the special environment makes their protein types, molecular weights, and contents significantly different, and they have special physiological and nutritional functions. As a "ship of the desert", camels have strong anti-fatigue propertie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/16
Inventor 邸多隆刘永峰
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI