Bottle-planted industrialized production and inoculation comprehensive disinfection method for pleurotus eryngii

Abstract

The invention relates to a bottle-planted industrialized production and inoculation comprehensive disinfection method for pleurotus eryngii. The method includes the following steps of transformation of a filtering system of an inoculation chamber, humidity and temperature control over the inoculation chamber, ozone and ultraviolet disinfection of air and mechanical equipment of the inoculation chamber, chlorine dioxide disinfector fumigation disinfection in the inoculation chamber, 75% ethyl alcohol disinfection and 95% ethyl alcohol flame sterilization, cleaning and hygiene after inoculationis finished in the inoculation chamber, and sterility detection of the inoculation chamber. After the comprehensive usage of the disinfection method above and determination of all kinds of disinfection and dust removal method parameters, the bottle-planted contamination rate is reduced from 10%o before the optimization to 2%o which is completely within a controllable value range; the defect that bottle-planted pleurotus eryngii is high in contamination rate is overcome, and a foundation is laid for stabilized production of pleurotus eryngii.

Description

technical field [0001] The invention relates to the technical field of bacterial control and disinfection of Pleurotus eryngii industrialized inoculation room, in particular to a comprehensive disinfection method for inoculation of Pleurotus eryngii bottle planted industrial production. Background technique [0002] The total output and annual output value of edible fungi in my country are increasing year by year. China is the world's largest producer and consumer of edible fungi, accounting for 70% of the world's total annual output. It has become the fifth largest crop in my country's agricultural field after grain, oil, fruit and vegetables. [0003] Among them, Pleurotus eryngii is a large fleshy toadstool, also known as Pleurotus eryngii. It is a rare new edible fungus variety that integrates edible, medicinal, and dietotherapy successfully developed and cultivated in recent years. Pleurotus eryngii is rich in nutrition, rich in protein, carbohydrates, polysaccharides, ...

AI Technical Summary

Problems solved by technology

[0004] At present, my country's industrial production of edible fungi has developed rapidly, and the production of industrialized Pleurotus eryngii in my country has increased rapidly in the total output. This is mainly because the output growth rate of bagged pleurotus eryngii is more than 80%. The quality and mushroom type of Pleurotus eryngii are at least one grade lower than those of bottle-grown Pleurotus eryngii. When the output of bag-grown Pleurotus eryngii is close to saturation, the market demand for high-quality Pleurotus eryngii is also increasing rapidly. Due to the limitation of investment level, technical level and research and development level, the bottle-cultivated production of Pleurotus eryngii in factory has encountered a bottleneck in the production process, and the industrial production process of bottle-grown Pleurotus eryngii is very easy to cause miscellaneous bacteria pollution. The main factor is The mouth of the bottle is large, and the contact area between the culture medium and the outside air is large during inoculation. The airtightness is not as good as that of Pleurotus eryngii in bags. The hygiene, disinfection, moisture, temperature, and high efficiency of the inoculation room are not properly controlled, which can easily cause inoculation pollution.