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Method for inducing epipremnum aureum calluses from leaves

A technology of callus and callus induction, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve problems such as large-scale stem rot, economic loss of pothos, and susceptible bacteria

Inactive Publication Date: 2018-01-26
东方上彩现代农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The propagation of pothos is currently carried out by cuttings. Due to the high temperature and high humidity in the growing environment of pothos, the offspring of cuttings are very susceptible to pathogens. Large-scale stem rot and root rot often occur in production, causing certain economic losses to the cultivation of pothos

Method used

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  • Method for inducing epipremnum aureum calluses from leaves
  • Method for inducing epipremnum aureum calluses from leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0100] Step 1, select the mother plant and use 80% carbendazim 1000 times solution to irrigate the roots for 30 days, once every 10 days.

[0101] Step 2, taking the young leaves at the growth point of the mother plant;

[0102] In step 3, the young leaves are sterilized to obtain leaves of pothos.

[0103] Step 4, after trimming the leaves of the pothos, to obtain a sheet;

[0104] Step 5, performing mechanical damage on the sheet;

[0105] Step 6, inoculating the mechanically damaged sheet into a callus induction medium, and culturing under the conditions of a temperature of 25°C-28°C and a humidity of 50%-65%, to obtain a callus.

[0106] Step 7, transfer the callus to the subculture medium under the conditions of light intensity: 1500-20001x, light time: 12 hours / day, temperature: 25°C-28°C, humidity: 50%-65% Cultivate up and down; subculture once every 40 days, that is.

[0107] Configure the callus induction medium as follows:

[0108] 1. Configure improved MS mediu...

Embodiment 2

[0130] Step 1, select the mother plant and use 80% carbendazim 1000 times solution to irrigate the roots for 30 days, once every 10 days.

[0131] Step 2, taking the young leaves at the growth point of the mother plant;

[0132] In step 3, the young leaves are sterilized to obtain leaves of pothos.

[0133] Step 4, after trimming the leaves of the pothos, to obtain a sheet;

[0134] Step 5, performing mechanical damage on the sheet;

[0135] Step 6, inoculating the mechanically damaged sheet into a callus induction medium, and culturing under the conditions of a temperature of 25°C-28°C and a humidity of 50%-65%, to obtain a callus.

[0136] Step 7, transfer the callus to the subculture medium under the conditions of light intensity: 1500-20001x, light time: 12 hours / day, temperature: 25°C-28°C, humidity: 50%-65% Cultivate up and down; subculture once every 40 days, that is.

[0137] Configure the callus induction medium as follows:

[0138]1. Configure improved MS medium...

Embodiment 3

[0160] Step 1, select the mother plant and use 80% carbendazim 1000 times solution to irrigate the roots for 30 days, once every 10 days.

[0161] Step 2, taking the young leaves at the growth point of the mother plant;

[0162] In step 3, the young leaves are sterilized to obtain leaves of pothos.

[0163] Step 4, after trimming the leaves of the pothos, to obtain a sheet;

[0164] Step 5, performing mechanical damage on the sheet;

[0165] Step 6, inoculating the mechanically damaged sheet into a callus induction medium, and culturing under the conditions of a temperature of 25°C-28°C and a humidity of 50%-65%, to obtain a callus.

[0166] Step 7, transfer the callus to the subculture medium under the conditions of light intensity: 1500-20001x, light time: 12 hours / day, temperature: 25°C-28°C, humidity: 50%-65% Cultivate up and down; subculture once every 40 days, that is.

[0167] Configure the callus induction medium as follows:

[0168] 1. Configure improved MS mediu...

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Abstract

The invention provides a method for inducing epipremnum aureum calluses from leaves. The method comprises the following steps: selecting parent plants, pretreating, and collecting epipremnum aureum leaves; performing mechanical damage to the epipremnum aureum leaves, and inoculating the epipremnum aureum leaves into a callus induction culture medium so as to obtain calluses; transferring the calluses into a subculture medium, and performing culture, thereby obtaining epipremnum aureum calluses. By adopting the method, epipremnum aureum sterile seedlings can be cultured by using an epipremnum aureum tissue culture technique, the growth cycle is shortened, loss of large-scale stalk rotting and root rotting caused by adverse factors is avoided or reduced, and the problems that epipremnum aureum is reproduced through cuttage at present, descendants of cuttage reproduction can be easily affected by bacteria, phenomena of large-scale stalk rotting and root rotting can be frequently caused inproduction and certain economic loss can be caused for epipremnum aureum culture are avoided.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for using leaves to induce callus of pothos. Background technique [0002] Green dill (scientific name: Epipremnum aureum), belonging to the genus Eucheuma, is a large evergreen vine that grows in tropical regions. It often climbs and grows on rocks and tree trunks in the rainforest. The petioles on mature branches are thick, 30-40 cm long, the base is slightly enlarged, the upper joints are 2.5-3 cm long, slightly hypertrophic, the ventral surface is wide grooved, the leaf sheath is long, the leaves are thin leathery, emerald green, usually (especially the leaves) There are many irregular pure yellow plaques, entire margins, ovate or ovate-oblong on unequal sides, short and acuminate at the apex, deeply cordate at the base, slightly thicker, and slightly raised on both sides. [0003] The origin of pothos is in the tropical rainforest of the Solomon Islands i...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王健刘坤申亮施金玉卢迪浩郑德助李健
Owner 东方上彩现代农业有限公司
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