Multi-channel automatic quantitative sterile sampling device and bioreactor with same

Abstract

The invention discloses a multi-channel automatic quantitative sterile sampling device and a bioreactor with the same. The multi-channel automatic quantitative sterile sampling device comprises a plurality of sampling pipes, first peristaltic pumps, second peristaltic pumps, first heating and cooling units, a main gas pipe and a first pinch valve, wherein the sampling ends of the sampling pipes are positioned inside different bioreactors, and the other ends of the sampling pipes are sample outlets; one first peristaltic pump is arranged on each sampling pipe, and all first peristaltic pumps are positioned between the bioreactors and the sample outlets; one second peristaltic pump is arranged on each sampling pipe, and all second peristaltic pumps are positioned between the first peristaltic pumps and the sample outlets; one first heating and cooling unit is arranged on each sampling pipe, and all first heating and cooling units are positioned between the second peristaltic pumps and the sample outlets; one end of the main gas pipe is connected with an air filter, and a plurality of branched gas pipes are arranged at the other end of the main gas pipe; each branched gas pipe is communicated with one sampling pipe, and all conjunctions are positioned between the first peristaltic pumps and the second peristaltic pump; the first pinch valve is arranged on the main gas pipe, and ispositioned between the air filter and the branched gas pipes.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a multi-channel automatic quantitative aseptic sampling device and a bioreactor with the device. Background technique [0002] A bioreactor is a device system that uses the biological functions of enzymes or organisms (such as microorganisms) to perform biochemical reactions in vitro. It is a biological function simulator, such as a fermenter, enzyme or cell reactor. It has important applications in wine, pharmaceutical production, concentrated jam, fruit juice fermentation, and degradation of organic pollutants. [0003] Sampling operations are often required during biological cultivation in bioreactors. This operation usually needs to meet the requirements of sterility, control of sampling volume, and difference control of multiple sampling. These functions are particularly important when the volume of culture medium is small or the sampling frequency is high and the cycle is lon...

AI Technical Summary

Problems solved by technology

[0006] 1. Duplication of devices brings about an increase in the cost of purchasing devices;

[0007] 2. The parameter setting of each channel needs to be repeated many times from the beginning to the end;

[0008] 3. The pipeline is simple and repetitive, and the pipeline is complicated;

[0009] 4. When used on a multi-channel microreactor device with a high degree of integration, the operation of the reactor will be difficult due to the excessive occupation of space

[0010] It can be seen from the above that the sampling device in the biological culture process in the above-mentioned prior art cannot meet the sampling requirements of multi-channel bioreactors

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