Method for quickly separating and preparing natural dihydroflavonoid compounds by using high-speed counter-current chromatography
A high-speed countercurrent chromatography and dihydroflavonoid technology is applied in the field of preparing dihydroflavonoids by a high-speed countercurrent chromatography continuous cycle separation method, which can solve the problems of long time consumption, single compound structure, and difficulty in one-time separation, etc. high purity effect
Active Publication Date: 2018-02-02
FUDAN UNIV
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Abstract
The invention belongs to the technical field of medicine, and relates to a method for separating and preparing natural dihydroflavonoid compounds. The method adopts a continuous circulation method ofhigh-speed counter-current chromatography, and a two-phase solvent system which is formed by n-hexane, ethyl acetate, methanol and water is adopted in the high-speed counter-current chromatography toseparate and prepare the dihydroflavonoid compounds from roots of sophora alopecuroide. According to the method, a mode of high-speed counter-current circulating elution, an online storage technique and a reverse elution mode are adopted at the same time, and continuous circulating elution are conducted on flow components which contain the target compound to collect the flow components and obtainflavonoid compounds which comprise sophoratonkin, alopecurone F, lehmannin, alopecurone A, sophora flavanone G and alopecurone B and have the purity of more than 95%; the five dihydroflavonoid compounds and santilin glycoside which have high purity are obtained through one-time separation, the method is simple, convenient and rapid in operation, and no loss of the target compounds is caused by material adsorption during the separation.
Application Domain
Sugar derivativesSugar derivatives preparation
Technology Topic
FlavanoneCounter current +10
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