Method for quickly separating and preparing natural dihydroflavonoid compounds by using high-speed counter-current chromatography

Abstract

The invention belongs to the technical field of medicine, and relates to a method for separating and preparing natural dihydroflavonoid compounds. The method adopts a continuous circulation method ofhigh-speed counter-current chromatography, and a two-phase solvent system which is formed by n-hexane, ethyl acetate, methanol and water is adopted in the high-speed counter-current chromatography toseparate and prepare the dihydroflavonoid compounds from roots of sophora alopecuroide. According to the method, a mode of high-speed counter-current circulating elution, an online storage technique and a reverse elution mode are adopted at the same time, and continuous circulating elution are conducted on flow components which contain the target compound to collect the flow components and obtainflavonoid compounds which comprise sophoratonkin, alopecurone F, lehmannin, alopecurone A, sophora flavanone G and alopecurone B and have the purity of more than 95%; the five dihydroflavonoid compounds and santilin glycoside which have high purity are obtained through one-time separation, the method is simple, convenient and rapid in operation, and no loss of the target compounds is caused by material adsorption during the separation.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a method for separating and preparing natural dihydroflavonoids, in particular to the preparation of dihydroflavonoids by adopting a high-speed countercurrent chromatography (HSCCC) continuous circulation separation method. Background technique [0002] The prior art discloses that Sophora alopecuroides L. is a perennial herb of the genus Sophora in the family Fabaceae, also known as Sophora alopecuroides. It is a commonly used Chinese medicinal material in Northwest my country, and its roots, stems, whole plants and seeds can be used as medicine. Bitter beans are bitter in taste and cold in nature, and have the functions of expelling wind and dampness, clearing heat and detoxification, relieving pain and killing insects. Pharmacological studies have shown that various types of compounds isolated from Sophora Sophora have pharmacological effects such as anti-tumor, immune regulati...

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Problems solved by technology

It has been reported in the literature that flavonoids, such as diosmin, luteolin, and 5,7-dihydroxychromanone, were isolated and purified from peanut shells using conventional HSCCC technology (Niu Dandan et al., Natural Product Research and Development, 2011,23:110-113), but the conventional HSCCC method takes a long time and the structure of the separated compound is relatively single. For Sophora sophora, it is difficult to quickly and simultaneously separate high-purity, complex and similar monomeric flavonoids Compounds

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