Application of lncRNAs as active tuberculosis specific markers

Abstract

The invention discloses application of lncRNAs as active tuberculosis specific markers. Due to tissue specificity and spatial-temporal specificity of lncRNAs, different tissues are different in lncRNAs expression quantity, and the same tissue or organ varies in lncRNAs expression quantity in different growth stages. Two lncRNAs serving as the active tuberculosis specific markers are disclosed forthe first time, a whole blood sample which can be acquired easily, directly and clinically is combined with designed primer pairs to verify lncRNAs differential expression through RT-qPCR, and quickness, simplicity, time saving, high detection sensitivity and low sample consumption are realized. The method is quite suitable for active tuberculosis diagnosis by lncRNAs in differential expression, and important significances to researching of tuberculosis propagation control and reduction of the tuberculosis occurrence rate are achieved.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a method for preparing lncRNAs specific to active pulmonary tuberculosis. Background technique [0002] Tuberculosis is a major global public health problem that seriously threatens human health. Pulmonary tuberculosis is the most common clinical type of tuberculosis. Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis infection. According to the World Health Organization (WHO), in 2015, there were about 10.4 million new cases of tuberculosis worldwide, 480,000 new cases of multidrug-resistant tuberculosis, and 1.4 million deaths from tuberculosis. The number of cases in China ranks second in the world. It is estimated that nearly 550 million people are infected with tuberculosis, and 1 million new cases of tuberculosis occur every year. The mortality rate was 2.32 / 100,000. Since tuberculosis is mainly airborne and has high morbidity a...

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Problems solved by technology

LncRNAs promoters can also bind transcription factors, such as Oct3 / 4, Nanog, CREB, Sp1, c-myc, Sox2 and p53, and local chromatin histones also have characteristic modification methods and structural features, with low sequence conservation , only about 12% of lncRNAs can be found in other organisms other than humans, and its mechanism of action is very complex and has not yet been fully understood. The lncRNAs are not clear

In addition, some online websites such as the starBase 2.0 website have low prediction accuracy. For example, GAS5 and miRNA21, which are now relatively clear, cannot be predicted, and there are as many as 1,400 lncRNAs in human serum, and there are still certain changes, resulting in the ineffective use of lncRNAs Therefore, for active pulmonary tuberculosis, developing appropriate specific lncRNAs as markers has important research and clinical significance in regulating gene transcription and as disease diagnostic markers.

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