Kit and method for methylation detection of cervical high-grade cervical lesions and cervical cancer related genes

A detection method and technology for cervical lesions, applied in the field of biomedicine, can solve problems such as difficulty in establishing low-resource areas, waste of medical resources, and transient infection, and achieve the effects of reducing mental burden, convenient sample collection, and strong specificity

Inactive Publication Date: 2018-02-02
上海捷诺生物科技有限公司
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the missed diagnosis rate of VIA is high, and the specificity of cytology is high, but the sensitivity is low, and the establishment of cytology depends on local health resources, and it is difficult to establish in low-resource a

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit and method for methylation detection of cervical high-grade cervical lesions and cervical cancer related genes
  • Kit and method for methylation detection of cervical high-grade cervical lesions and cervical cancer related genes
  • Kit and method for methylation detection of cervical high-grade cervical lesions and cervical cancer related genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0042] The present invention will be further described below in conjunction with the examples. It should be understood that the examples are only used to further illustrate and illustrate the present invention, and are not intended to limit the present invention. Materials, reagents, etc. used in the following examples, unless otherwise noted, all reagents were obtained commercially.

[0043] The cervical lesion and cervical cancer-related gene methylation detection kit provided by the present invention, as shown in Table 1, includes the following components:

[0044] Table 1 Kit components

[0045]

[0046] Eight pairs of primers are immobilized inside the eight PCR tubes, as shown in Table 2, including six pairs of cervical lesion markers DLX1 (homologous transcription factor 1), ITGA4 (integrin α4, integrin subunit alpha 4), RXFP3 (relaxed Protein family receptor 3, relaxin / insulin like family peptide receptor 3), SOX17 (SRY-Box 17), ZNF671 (zinc finger protein 671, zin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a kit and method for methylation detection of cervical lesions and cervical cancer related gene. The kit contains reagents including a PCR reaction solution, eight primers fixed to a PCR octa-tube, a positive reference substance, a negative reference substance and a cell lysate for extracting a sample DNA. The detection method comprises the steps of extracting the sample DNA, processing and purifying the sample DNA, preparing a PCR reaction system and adding the sample, carrying out a PCR amplification reaction, collecting a fluorescence signal, obtaining and analyzingan amplification curve and a melting curve, obtaining a Ct value and a Tm value of each marker by analyzing the amplification curve and the melting curve, formulating a positive determination method based on a weighting algorithm, carrying out positive determination on each marker and integrating, finally explaining sample results. The kit has the advantages of high sensitivity, strong specificityand non-invasiveness, and is particularly suitable for auxiliary diagnosis of cervical high-grade lesions or cervical cancer and HPV-positive shunt management.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a kit and method for detecting methylation of cervical high-grade lesions and cervical cancer-related genes. Background technique [0002] Cervical cancer is one of the common female malignant tumors, its incidence is second only to breast cancer, ranking second in gynecological tumors. According to the latest data released by the National Cancer Registry Center, there were 98,900 new cervical cancer cases and 30,500 deaths in my country in 2015, and the incidence and mortality rates are increasing year by year. Scientific research has confirmed that persistent infection of high-risk papillomavirus (hrHPV) is the main cause of cervical cancer, and HPV-negative women have an extremely low risk of cervical cancer. However, most women who test positive for HPV do not develop precancer or cancer, and only a minority of women with HPV infection are at risk of developi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/154C12Q2600/166
Inventor 程天龄杜金凤玛蒂娜施密茨
Owner 上海捷诺生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap