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Method for constructing fingerprint spectrum of anti-inflammatory disinfecting capsules

A fingerprint and capsule technology, applied in the field of fingerprint construction, can solve problems such as incomplete quality control indicators, achieve good feasibility, ensure normal production, and avoid product forgery.

Inactive Publication Date: 2018-02-13
LIUZHOU WORKERS HOSPITAL
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  • Description
  • Claims
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Problems solved by technology

For traditional Chinese medicine with complex ingredients, the past practice of using a single active ingredient as its quality control index is not comprehensive, and the analogy of "blind men feeling the elephant" cannot be more appropriate

Method used

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  • Method for constructing fingerprint spectrum of anti-inflammatory disinfecting capsules
  • Method for constructing fingerprint spectrum of anti-inflammatory disinfecting capsules
  • Method for constructing fingerprint spectrum of anti-inflammatory disinfecting capsules

Examples

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example 1

[0028] Example 1: The construction method of the fingerprint of anti-inflammatory disinfection capsule

[0029] 1. Experimental instruments and materials

[0030] LC‐10ATVP high performance liquid chromatography (Shimadzu, Japan);

[0031] Chromatographic column (phenomenex Synergi Fusion-RP C18 (250mm×4.6mm, 4um) chromatographic column);

[0032] Acetonitrile (chromatographically pure);

[0033] Methanol (analytical pure);

[0034] Phosphoric acid (analytical pure);

[0035] Ammonia water (analytical pure, 25%-28%);

[0036] Reference substance: chlorogenic acid, luteolin, isoxazinidine, dehydroandrographolide reference substance;

[0037] Test product: anti-inflammatory disinfectant capsule sample.

[0038] 2. Chromatographic conditions

[0039] phenomenex Synergi Fusion-RP C18 (250mm×4.6mm, 4um) chromatographic column; acetonitrile (A)-0.4% phosphoric acid solution (B) as mobile phase, flow rate 1.0mL·min-1, gradient elution: 0→20min , the volume ratio of mobile pha...

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Abstract

The invention aims to provide a fingerprint spectrum of anti-inflammatory disinfecting capsules. Multi-target ingredients of the anti-inflammatory disinfecting capsules are detected by high performance liquid chromatography, a chromatogram with 16 characteristic peaks is constructed, and the sixth peak serves as a reference peak, so that relative retention time and relative peak area of the commonpeak are calculated. By constructing the fingerprint spectrum of the anti-inflammatory disinfecting capsules, an effective and reliable quality control method of the anti-inflammatory disinfecting capsules and application thereof can be provided.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a method for constructing fingerprints of anti-inflammatory disinfectant capsules. Background technique [0002] Inflammation is mainly caused by bacteria and viruses, manifested as fever, headache, sore throat, muscle aches, and joint pains all over the body. Traditional Chinese medicine and traditional Chinese medicine can treat both symptoms and root causes, and comprehensively regulate all aspects of inflammation. According to folk plant anti-inflammatory drugs, anti-inflammatory and disinfecting capsules have been developed, which are composed of medicinal materials such as mountain silver flower, wild chrysanthemum, andrographis paniculata, and sarcocarpus, with remarkable clinical effects. [0003] The requirements of the international community for medicines are "safe, effective and controllable". In the current quality standard of traditional Chinese medicine, th...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 李锦燊吴洪文杨志杰曾瑜方芳
Owner LIUZHOU WORKERS HOSPITAL
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