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Use of sarpogrelate hydrochloride impurities I for inducing differentiation of bone marrow mesenchymal stem cells

A technology of sarcogrelate hydrochloride and bone marrow mesenchyme, which is applied in the field of inducing bone marrow mesenchymal stem cell differentiation, sarcogrelate hydrochloride impurities, and can solve the problem of no pharmacological activity

Inactive Publication Date: 2018-02-23
南京普氟生物检测技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, sarcogrelate hydrochloride impurity I is mainly used for the quality control of sarcogrelate hydrochloride raw materials and preparations, and there is no report on its pharmacological activity

Method used

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  • Use of sarpogrelate hydrochloride impurities I for inducing differentiation of bone marrow mesenchymal stem cells
  • Use of sarpogrelate hydrochloride impurities I for inducing differentiation of bone marrow mesenchymal stem cells
  • Use of sarpogrelate hydrochloride impurities I for inducing differentiation of bone marrow mesenchymal stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Sargrelate Hydrochloride Impurity I Induces Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells (BMSCs)

[0021] 1. Experimental materials

[0022] Low-sugar DMEM medium (Gibco, USA), fetal bovine serum (Gibco, USA), 0.25% trypsin (containing 0.02% EDTA, Gibco, USA), alizarin red S (Sigma, USA), goat COLⅠ polyclonal antibody ( Santa Cruz, USA). Impurity Ⅰ of sargrelor hydrochloride is self-made, and the HPLC normalized purity is greater than 98%.

[0023] 2. Experimental method

[0024] 1. Isolation and culture of BMSCs

[0025] Take 2 healthy SD rats aged 3 weeks, weighing about 100g, male or female, given an excessive amount of 10% chloral hydrate for intraperitoneal injection to death, soaked in 75% alcohol for 10min, and double-dipped the rats under aseptic conditions. Take out the tibia and femur on the side, and remove the attached tissues. When removing the cartilage tissue at the joint and the bone ends on both sides, pay attention to ...

Embodiment 2

[0047] The preparation of embodiment 2 solid dispersion

[0048] Dissolve sarcogrelate hydrochloride impurity I and povidone K-30 in absolute ethanol according to the following mass ratio to prepare a solution with a mass concentration of sarcogrelate hydrochloride impurity I of 10mg / mL, and stir magnetically at room temperature for 4h (100r / min) , the solvent was removed by rotary evaporation under reduced pressure at 50°C, dried in vacuum for 24 hours, crushed through a No. 5 sieve, and stored in a desiccator for future use.

[0049] Solid dispersion 1: the mass ratio of sarcogrelate hydrochloride impurity I to povidone K-30 is 1:5;

[0050] Solid dispersion 2: the mass ratio of sarcogrelate hydrochloride impurity I to povidone K-30 is 1:4.5;

[0051] Solid dispersion 3: the mass ratio of sarcogrelate hydrochloride impurity I to povidone K-30 is 1:5.5;

[0052] Solid dispersion 4: the mass ratio of sarcogrelate hydrochloride impurity I to povidone K-30 is 1:4;

[0053] So...

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Abstract

The invention discloses the use of sargrelate hydrochloride impurity I for inducing differentiation of bone marrow mesenchymal stem cells. The present invention provides the application of sarcogrelate hydrochloride impurity I in the preparation of drugs for inducing osteogenic differentiation of bone marrow mesenchymal stem cells. The cognition level of prior art to sarcogrelate hydrochloride impurity I; In order to overcome the problem of poor water solubility and low bioavailability of sarcogrelate hydrochloride impurity I-povidone K ‑30 solid dispersion, the preparation can significantly improve the water solubility of impurity Ⅰ of sarcogrelate hydrochloride, thereby improving its bioavailability.

Description

technical field [0001] The invention belongs to the field of medicines, and relates to new applications of known compounds, in particular to the application of a sarcogrelate hydrochloride impurity I for inducing differentiation of bone marrow mesenchymal stem cells. Background technique [0002] Sagrelate Hydrochloride Tablets was first launched in Japan in 1993 under the trade name of Anplag. It is a 5-HT2 receptor blocker that can inhibit platelet aggregation and vasoconstriction, has antithrombotic effect and improves microcirculation. Its indications are to improve ischemic symptoms such as ulcers, pain and cold sensation caused by chronic arterial occlusive disease. [0003] Sagrelate hydrochloride impurity I is a photodegradation impurity of sarcogrelate hydrochloride, and its chemical structure is as follows: [0004] [0005] In the prior art, sarcogrelate hydrochloride impurity I is mainly used for quality control of sarcogrelate hydrochloride raw materials and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/138A61K47/32A61P43/00
CPCA61K31/138A61K47/32
Inventor 杨豪伟张思静胡戈
Owner 南京普氟生物检测技术有限公司
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