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Pepsin detection kit and detection method

A pepsin and kit technology, applied in the field of pepsin detection, can solve the problems of cumbersome operation and long detection time, and achieve the effects of good sensitivity, easy operation and high precision

Inactive Publication Date: 2018-02-23
XIAMEN YIKELISI MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, it has been gradually believed that the detection of pepsin in saliva has a certain diagnostic value for gastroesophageal reflux, but ELISA and other pepsin detection methods are cumbersome to operate and take a long time to detect

Method used

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  • Pepsin detection kit and detection method
  • Pepsin detection kit and detection method
  • Pepsin detection kit and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Please check Figure 1 to Figure 3 , a pepsin assay kit, comprising a box body, a test strip A and a reagent bottle B are arranged in the box body;

[0033] The test strip A includes a housing 20 and a body 10 disposed in the housing 20; the body 10 includes a back substrate 11, on which a sample binding pad 12 is provided, and a nitrocellulose membrane 13 and Water-absorbing membrane 14, the sample binding pad 12 and water-absorbing membrane 14 are laminated on the two ends of the nitrocellulose membrane 13 respectively; It is obtained by immersing in the buffer solution and then dried or coated with the buffer solution and then dried. It contains pepsin inhibitor and pH buffer solution, which can terminate the reaction catalyzed by pepsin, and drop the liquid on the sample binding pad 12 The pH value is adjusted to the range suitable for the antigen-antibody reaction; the nitrocellulose membrane 13 is provided with a detection line (T line) 131, and the detection lin...

Embodiment 2

[0036] A pepsin assay kit, comprising a box body, a test strip A, a reagent bottle B and a reagent bottle C are arranged in the box body;

[0037] The test strip A includes a housing 20 and a body 10 disposed in the housing 20; the body 10 includes a back substrate 11, on which a sample binding pad 12 is provided, and a nitrocellulose membrane 13 and Water-absorbing membrane 14, the sample binding pad 12 and water-absorbing membrane 14 are respectively laminated on the two ends of the nitrocellulose membrane 13; the nitrocellulose membrane 13 is provided with a detection line (T line) 131, and the detection line 131 is coated with There is a monoclonal antibody against pepsin substrate, and the casing 20 is provided with a sample injection hole 21 at a position corresponding to the sample binding pad 12 , and an observation window 22 is opened at a position corresponding to the detection line 131 .

[0038] The reagent bottle B is filled with a reaction solution, which contain...

Embodiment 3

[0041] The present invention simultaneously provides a pepsin assay method, comprising:

[0042] 1) reacting the sample to be tested with the reaction solution, which contains a labeled pepsin substrate; the substrate should be far in excess relative to pepsin;

[0043] 2) After reacting for a certain period of time, the product of step 1) is digested, then mixed with the monoclonal antibody against the pepsin substrate, and the remaining labeled pepsin substrate reacts with the monoclonal antibody against the pepsin substrate, generating a labeled substrate-antibody complex;

[0044] 3) Detecting the amount of the labeled substrate-antibody complex, thereby performing indirect qualitative and / or quantitative detection of pepsin in the sample to be tested.

[0045] Preferably, the method for measuring pepsin using the kit of the present invention includes:

[0046] 1) React the sample to be tested with the reaction solution in the reagent bottle B, and the pepsin in the samp...

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Abstract

The invention discloses a pepsin detection kit and a detection method. The kit comprises a test strip and reaction liquid, wherein the test strip comprises a body which comprises a backing bottom plate; a sample binding pad, a nitrocellulose membrane and a water absorption membrane are arranged on the backing bottom plate; the sample binding pad and the water absorption membrane are respectively overlaid at two ends of the nitrocellulose membrane; a detection line is arranged on the nitrocellulose membrane, and is coated with a monoclonal antibody for resisting pepsin substrate; and the reaction liquid contains marked pepsin substrate. According to the pepsin detection kit, by utilizing the characteristic that the reactionogenicity of substrate is lost due to the protein digestion characteristic of pepsin, pepsin is indirectly qualitatively and / or quantitatively detected by detecting the quantity of the substrate with reactionogenicity remained after being digested by pepsin, so that the pepsin detection kit has the characteristics of good sensitivity, high precision and easy operation.

Description

technical field [0001] The invention belongs to the technical field of pepsin detection, and in particular relates to a pepsin detection kit and a detection method. Background technique [0002] Gastroesophageal reflux refers to a disease in which gastric contents such as gastric juice flow back into the esophagus, throat, and even the mouth. It often causes symptoms such as heartburn, chest pain, foreign body sensation in the throat, and coughing. It is one of the common gastroenterological diseases by damaging the mucosa of the tract, damaging the proteins between cells or on the cell membrane, and causing a series of complications. In recent years, it has been gradually believed that the detection of pepsin in saliva has a certain diagnostic value for gastroesophageal reflux, but ELISA and other pepsin detection methods are cumbersome to operate and take a long time to detect. Therefore, it is of great significance to establish a detection method that can quickly detect ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558G01N33/533
Inventor 许元峰袁新吕怀谷袁玲赵静
Owner XIAMEN YIKELISI MEDICAL TECH CO LTD
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