Fifteen-channel microfluidic chip apparatus for general investigation of tumor markers

Abstract

The invention relates to a fifteen-channel microfluidic chip device for general screening of tumor markers, belonging to the field of analysis and testing. If PDMS, or polydimethylsiloxane, is used to make the substrate of a microfluidic chip for the joint detection of more than ten tumor markers, there are several technical difficulties. The main point of this case is that the substrate is selected from PDMS with an original ecological surface, and the link-type electromagnetic fixture equipped with a micro-ultrasonic transducer is socketed and positioned in the vicinity of the sample liquid flow terminal of the microfluidic chip. Ultrasonic waves are used to induce the sample liquid to flow from the sample injection end of the chip along the pipe of the chip to the terminal. The pipe of the chip is filled with micron-sized silica particle fillers. The function of the filler includes supporting the inner wall of the pipe. , and, use a collection of hydrophilic micro-channels to compensate the original hydrophobic pipeline, and squeeze and cover the inner wall surface of the pipeline so as to reduce the chance of biomacromolecules contacting the inner wall surface.

Description

technical field [0001] The invention relates to a fifteen-channel microfluidic chip device for general screening of tumor markers, belonging to the field of analysis and testing. Background technique [0002] Tumor markers (tumor markers, TM) refer to a class of substances produced by tumor cells themselves or produced by the body's response to tumor cells during the occurrence and proliferation of tumors, reflecting the existence and growth of tumors, including Proteins, hormones, enzymes (isoenzymes) and oncogene products, etc. Testing the tumor markers in the patient's blood or body fluids can detect tumors early in the tumor screening, observe the curative effect of tumor treatment and judge the prognosis of patients. Currently, the tumor markers commonly used clinically are: (1) alpha-fetoprotein (AFP) is a marker for primary liver cancer, testicular cancer, ovarian cancer and other tumors; (2) carcinoembryonic antigen (CEA) is a marker for digestive system tumors, (3...

AI Technical Summary

Problems solved by technology

[0008] But it's not that simple

[0009] First, this polydimethylsiloxane material, which is referred to by the acronym PDMS, is itself a strongly hydrophobic material, and channels are built on this material. modification operation, then, after the overall assembly is completed, that is, after the cover is covered, because the inner surface of the channel in the structure occupies most of the inner surface of the liquid flow channel, then the inner surface of the PDMS channel is The strong hydrophobicity is the decisive factor, it will make it very difficult for the polar liquid fine flow similar to the aqueous solution to pass through, and its flow resistance is so large that even the general micropump is difficult to push, of course, if the cover sheet is also If you choose to use the PDMS material, then the problems are basically the same, with minor differences; therefore, in the prior art, it is necessary to modify and modify the inner surface of the channel on the PDMS material; then, this is for the PDMS channel Is the modification operation of the inner surface of the channel very troublesome? That's not the problem. What constitutes a serious technical problem is another problem: the PDMS polymer molecules in the bulk phase of the PDMS material substrate have the characteristics of automatic diffusion and migration to the surface. The characteristics of polymer molecules diffusing and migrating to the surface automatically will make the modified state of the inner surface of the channel modified by the surface unable to maintain for a long enough time. The maintenance time of the internal surface state is roughly only enough to complete the time required for the internal test experiment in the laboratory; in other words, the surface state of the PDMS channel inner surface after surface modification or surface modification is formed after modification It cannot last for a long time, but it will automatically tend to or change back to the surface state before the surface modification, and return to the original strongly hydrophobic surface state in a short period of time. Then, just imagine, such a slight Can flow control chips be produced in large quantities, stored in large quantities, and widely promoted? The answer is obvious, that is, impossible

This problem has also existed for many years, and so far, it has not been properly solved

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