Detecting method for unicellular mass spectrum flow cytometry

A flow cytometry and single-cell technology, applied in the field of biological inspection or detection, can solve the problems of detection efficiency not meeting high-throughput detection, cumbersome operation, interference, etc.
CN107796748AInactive Publication Date: 2018-03-13SHANGHAI JIAO TONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI JIAO TONG UNIV
Publication Date
2018-03-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a detecting method for unicellular mass spectrum flow cytometry. A novel encoding method of an isotope metal concentration ratio marking antibody is applied, metals with different ratios is used, thus a new label combination is structured; thus a detecting channel of the mass spectrum flow cytometry is expanded by a few metal label number, a Barcoding technology which moreefficient than the existed technology is developed.
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Description

technical field

[0001] The present invention belongs to the field of biotechnology, and in particular the present invention relates to biological inspection or detection, using cell Barcoding technology to pre-mark cells before single-cell mass flow cytometry, and to analyze the group to which each cell belongs after mass spectrometry method. Background technique

[0002] Mass cytometry (Mass Cytometry) is a flow cytometry technique that uses the principle of mass spectrometry to perform multi-parameter detection of single cells. It inherits the high-speed analysis characteristics of traditional flow cytometry, and has the high-resolution capability of mass spectrometry detection, which is a new development direction of flow cytometry. It can perform fine immunophenotyping and signaling pathway analysis on complex samples; automatically group immune cells and perform detailed analysis on the functional polymorphism of immune cells; it can perform fine subgroup analysis on c...

Claims

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