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Method for carrying out in-vitro culture on zygophyllum xanthoxylum

A technology of in vitro culture and solid medium, applied in the field of in vitro culture of Bawang, which can solve the problems of low survival rate, season-affected reproduction of Bawang, long seedling cycle, etc., to achieve short reproduction cycle, simple operation, and high genetic stability Effect

Active Publication Date: 2018-03-16
GANSU DESERT CONTROL RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the purpose of the present invention is to provide a method for overlord in vitro cultivation, to solve the problems in the prior art that overlord breeding is affected by seasons, the survival rate is low, and the seedling cycle is long

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Seed disinfection: peel off the seeds from the capsule of Bawang, rinse with running water for 20 minutes, then disinfect with 75% alcohol for 10 minutes, rinse with sterile water 5 times, each time for 1 minute; then use 0.1% HgCl 2 After the solution sterilized the seeds deeply for 10 minutes, they were rinsed with sterile water for 5 times, each time for 1 minute; finally, the moisture on the surface of the seeds was blotted with sterile filter paper.

[0033] (2) Aseptic seedling cultivation: inoculate the sterilized seeds on the surface of 1 / 2MS solid medium (the content of each component of the conventional MS medium is halved), the medium also contains 15g / L sucrose, 8g / L agar, The pH value is 5.8. The diameter of the culture bottle is 5cm, and 4 seeds are inoculated in each bottle. Placed at 25°C and cultivated under light conditions for 12 days, the height of the sterile seedlings reached 4.5 cm, with 2 lateral branches, and the length of the lateral branc...

Embodiment 2

[0038] (1) Seed disinfection: peel off the seeds from the capsule of Bawang, rinse with running water for 22 minutes, then disinfect with 75% alcohol for 12 minutes, rinse with sterile water 5 times, each time for 1 minute; then use 0.1% HgCl 2 After the solution sterilized the seeds deeply for 10 minutes, they were rinsed with sterile water for 5 times, each time for 1 minute; finally, the moisture on the surface of the seeds was blotted with sterile filter paper.

[0039] (2) Aseptic seedling cultivation: inoculate the sterilized seeds on the surface of 1 / 2MS solid medium (the content of each component of the conventional MS medium is halved), the medium contains 15g / L sucrose, 8g / L agar, pH is 5.8. The diameter of the culture bottle is 5cm, and 3 seeds are inoculated in each bottle. Placed at 25°C and cultivated under light conditions for 10 days, the height of the sterile seedlings reaches 5cm, 2 side branches, and the length of the side branches is 3cm;

[0040] (3) Clu...

Embodiment 3

[0044] (1) Seed disinfection: peel off the seeds from the capsule of Bawang, rinse with running water for 18 minutes, then disinfect with 75% alcohol for 12 minutes, rinse with sterile water 5 times, each time for 1 minute; then use 0.12% HgCl 2 After the solution sterilized the seeds deeply for 10 minutes, they were rinsed with sterile water for 5 times, each time for 1 minute; finally, the moisture on the surface of the seeds was blotted with sterile filter paper.

[0045] (2) Aseptic seedling cultivation: inoculate the sterilized seeds on the surface of 1 / 2MS solid medium (the content of each component of the conventional MS medium is halved), the medium contains 15g / L sucrose, 8g / L agar, pH is 5.8. The diameter of the culture bottle is 5cm, and 5 seeds are inoculated in each bottle. Placed at 25°C and cultured under light conditions for 15 days, the height of the sterile seedlings reached 4 cm, and no side branches grew out.

[0046] (3) Cluster bud induction: Cut the st...

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Abstract

The invention provides a method for carrying out in-vitro culture on zygophyllum xanthoxylum. The method comprises the following steps: (1) enabling zygophyllum xanthoxylum seeds to be flushed by flowing water, soaked and disinfected by alcohol, soaked and disinfected by an HgCl2 solution and flushed by aseptic water in sequence, thus obtaining disinfected seeds; (2) inoculating the disinfected seeds into a one-half MS (Murashige Skoog) solid culture medium, and culturing for 10 to 15 days, thus obtaining aseptic seedlings; (3) obtaining stems with leaf axils from the aseptic seedlings, inoculating the stems into a cluster bud induction culture medium, and culturing for 15 to 20 days, thus obtaining cluster buds; (4) inoculating the cluster buds obtained in the step (3) into a rooting culture medium, and culturing for 10 to 15 days, thus obtaining tissue cultured seedlings. The method provided by the invention has the advantages of simpleness in operation, high genetic stability and short reproductive cycle.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for in vitro culture of Bawang. Background technique [0002] Overlord (Zygophyllum xanthoxylum) is a polyphytic xerophyte of Tribulus terrestae widely distributed in the arid desert area of ​​Northwest my country. It has super drought resistance and contains abundant drought resistance gene resources. Bawang mostly grows on arid sandy land, gravel land and sand-covered land. It is one of the main dominant species and construction species of desert shrub vegetation. It has strong stress resistance and great ecological plasticity. The young branches and leaves of Bawang Both flowers and flowers are eaten by camels, and have good feeding value and palatability. Bawang Grassland is the main grazing land in Northwest my country. However, the Bawang Grassland in my country is mostly ecologically fragile, especially because of long-term overgrazing an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 张莹花尉秋实王方琳李得禄
Owner GANSU DESERT CONTROL RES INST