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A method for detecting reduced glutathione or triglyceride

A glutathione and triglyceride technology, applied in the field of electrochemistry, can solve problems such as complex operations and expensive equipment, and achieve the effects of low cost, convenient and fast detection, and simple operation

Active Publication Date: 2020-01-07
NORTHWEST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods require expensive equipment and complicated operations

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  • A method for detecting reduced glutathione or triglyceride
  • A method for detecting reduced glutathione or triglyceride
  • A method for detecting reduced glutathione or triglyceride

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Embodiment 1

[0040] This embodiment provides a method for detecting reduced glutathione, and the schematic diagram of the principle of glutathione determination is shown in figure 1 .

[0041] Prepare a NADPH solution with a concentration of 2-15mM in the HEPES buffer solution, and detect it separately with a blood glucose meter to obtain a linear relationship between the NADPH concentration and the response value of the blood glucose meter, see figure 2 .

[0042] (1) Detection steps of reduced glutathione in buffer solution:

[0043] Solution A: Mix 10 mM NADPH, 40 U / mL glutathione reductase, 20 U / mL glutathione peroxidase, 50 mMATP, 50 U / mL hexokinase, and 10 mM hydrogen peroxide; mix 8 µL of solution A with 2 µL The HEPES buffer solution was mixed and tested with a blood glucose meter, and the result was recorded as P 1 ; Prepare glutathione solution with a concentration range of 0.1mM to 2mM in HEPES buffer solution, mix 8μL solution A with 2μL glutathione solution, let it stand f...

Embodiment 2

[0049] This embodiment provides a method for detecting triglycerides. The principle schematic diagram of triglycerides determination is shown in Figure 6 .

[0050] (1) Detection steps of triglycerides in buffer solution:

[0051] Dissolve triglycerides in a mixture of 6 mg lecithin and 80 μL chloroform, and dilute to different concentrations with tris-hydrochloric acid buffer solution, and mix the obtained triglyceride solution with 50 U / mL lipase, 50 mM Mix ATP and 50U / mL hexokinase mixture, shake the reaction, take 5 μL of the reaction product and 15 μL 33mM ammonium sulfate, 15mM NAD + Mix with 50U / mL glycerol dehydrogenase, let it stand for 20min, and measure the results with a blood glucose meter to obtain the relationship between the triglyceride concentration and the response value of the blood glucose meter, see Figure 7 , The detection range of triglycerides by the blood glucose meter is 0.5-1.5mM, and the detection limit is 0.17mM.

[0052] (2) Detection steps ...

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Abstract

The invention discloses a method for detecting reduced glutathione or triglyceride, which comprises the following steps: step 1, respectively preparing precursor solutions for detecting reduced glutathione or triglyceride, and standing still for 10-20 minutes ; Step 2, use the blood glucose meter to detect the precursor solution, and obtain the concentration of reduced glutathione and triglyceride respectively through the response value of the blood glucose meter. The present invention explores a new method for detecting reduced glutathione and triglyceride, which can be detected by using a commonly used blood glucose meter, and the detection range of reduced glutathione solution is 0.24mM~1.5mM , the detection range of triglyceride is 0.5mM~2.0mM, and the detection of non-glucose target objects is realized by using the blood glucose meter.

Description

technical field [0001] The invention belongs to the technical field of electrochemistry, and in particular relates to a method for detecting glutathione and triglyceride by a blood glucose meter. Background technique [0002] In recent years, the development of portable and cost-effective equipment to detect some disease markers is the research direction of many researchers. This equipment allows everyone to quickly and conveniently detect some physical indicators at home and detect diseases as early as possible. It is convenient for people living in remote mountainous areas with underdeveloped medical care. To achieve this goal, researchers have made great efforts, however, only limited parts are available to the public. The most successful example so far is the blood glucose meter, which can be bought in pharmacies everywhere or as an accessory for smartphones. However, current blood glucose meters are only used to detect blood sugar. Therefore, it is very necessary to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26
CPCG01N27/26
Inventor 盛庆林许锦琼郑建斌
Owner NORTHWEST UNIV