Method for extracting schaftoside from herba desmodii styracifolii

A technology of Radix Polygonum vulgaris and schaffatin, which is applied in the extraction field of the biotechnology field, can solve the problems of low solubility, harsh equipment requirements, and low selectivity.

Inactive Publication Date: 2018-03-27
桂林纽泰生物科技有限公司
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the common solvent C0 2 It has the characteristics of non-polarity and small relative molecular mass, which makes the extraction effect unsatisfactory due to its low solubility and low selectivity for some substances. In actual operation, it is often necessary to increase the extraction rate by adding entraining agents. Finally, it is necessary to try to remove the entraining agent in the volatile oil, which will inevitably lead to the complexity of the process and the loss and oxidation of volatile components; in addition, because water is insoluble in CO 2 , so the supercritical C0

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting schaftoside from herba desmodii styracifolii
  • Method for extracting schaftoside from herba desmodii styracifolii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting schaftoside from Desmodium sativa, specifically comprising the steps of:

[0025] 1) Clean Desmodium sativa, dry, pulverize with a pulverizer, and cross a 20-mesh sieve;

[0026] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 150-160°C, pressure 8-10Mpa, extraction time 0.5h, extract Desmodium sativa, after the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature to obtain the extract;

[0027] 3) Purification: Concentrate the extract obtained in step 2), control the temperature ≤ 50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, perform column chromatography through DM130 macroporous resin, and load the s...

Embodiment 2

[0029] A method for extracting schaftoside from Desmodium sativa, specifically comprising the steps of:

[0030] 1) Clean Desmodium sativa, dry, pulverize with a pulverizer, and pass through a 40-mesh sieve;

[0031] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 150-160°C, pressure 8-10Mpa, extraction time 1.0h, extract Desmodium sativa, after the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature to obtain the extract;

[0032] 3) Purification: Concentrate the extract obtained in step 2), control the temperature to ≤50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, and perform column chromatography through AB-8 macroporous resin, T...

Embodiment 3

[0034]A method for extracting schaftoside from Desmodium sativa, specifically comprising the steps of:

[0035] 1) Clean Desmodium sativa, dry, pulverize with a pulverizer, and cross an 80-mesh sieve;

[0036] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 150-160°C, pressure 8-10Mpa, extraction time 1.0h, extract Desmodium sativa, after the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature to obtain the extract;

[0037] 3) Purification: Concentrate the extract obtained in step 2), control the temperature ≤ 50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, perform column chromatography through D101 macroporous resin, and load the sa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for extracting schaftoside from herba desmodii styracifolii. The method comprises the following steps: (1) cleaning and air drying the herba desmodii styracifolii, pulverizing the herba desmodii styracifolii by using a pulverizing machine, and sieving; (2) subcritical water extracting: placing the processed raw material in an extraction kettle, adding hydroxyethylcellulose equivalent to 0.001 parts by weight of the raw material, injecting deoxygenated deionized water into the kettle, wherein the extraction temperature is 150 to 160 DEG C, the pressure is 8 to10Mpa, the extraction time is 0.5 to 1.0h, extracting the herba desmodii styracifolii, after the extraction is ended, releasing the pressure, enabling extract liquid to flow into a collection tank, cooling to room temperature, and obtaining the extract liquid; and (3) purifying: concentrating the extract liquid obtained in step (2), performing the column chromatography by virtue of macroporous resin, eluting by using 50 percent by volume of ethanol, obtaining eluent, concentrating, and drying, thus obtaining a finished product.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting schaftoside from Desmodium sativa. Background technique [0002] Schaftoside, molecular formula: C 26 h 28 o 14 , is the apigenin flavonoid carbon glycosides in Desmodium sativa, and is an important active ingredient for its anti-inflammatory, heat-clearing and damp-dampening, diuretic and stranguria-relieving effects. It is recorded in "Chinese Pharmacopoeia" and "Dictionary of Traditional Chinese Medicine" that Desmodium radix mainly contains alkaloids, flavonoids, phenols, tannins and other components. [0003] Herba Desmodium styracifolii (Herba Desmodii styracifolii) is a commonly used Chinese herbal medicine. It is the aboveground dry part of Desmodium styracifolium (Osh.) Merr. in the leguminous plant, and it is included in the 2010 edition of Pharmacopoeia of the People's Republic of China. The original plant is a semi-shrub...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07D407/14
CPCC07D407/14
Inventor 谢冬养
Owner 桂林纽泰生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap