Detection system of IDH1/2 gene mutation and kit thereof

Abstract

TThe invention relates to a detection system of IDH1 / 2 gene mutation and a kit thereof. The kit comprises PCR detection liquid and SYBR GreenI mixed liquor; the PCR detection liquid A comprises a forward primer A and a reverse primer A and peptide nucleic acid A which are used for detecting a 394th codon C394T site and a 395th codon G395A site of IDH1 / 2 gene, the PCR detection liquid B comprises aforward primer B and a reverse primer B and peptide nucleic acid B which are used for detecting a 419th codon G419A site of IDH1 / 2 gene, and the PCR detection liquid C comprises a forward primer C and a reverse primer C and peptide nucleic acid C which are used for detecting a 515th codon G515A site of IDH1 / 2 gene. The IDH 1 / 2 gene mutation detection system and the kit thereof have high sensitivity and specificity, sample demand is low, and the system and the kit can be used for rapidly, conveniently and accurately carrying out mutation detection of IDH1 / 2 gene.

Description

technical field [0001] The invention relates to a method for detecting IDH1 / 2 gene mutation and a detection product, belonging to the field of biotechnology. Background technique [0002] In 2008, Parsons et al conducted a high-throughput expression analysis study on 20,661 protein-coding genes, and found for the first time that about 12% of glioblastomas had the gene of isocitrate dehydrogenase 1 (IDH1) Subsequently, further studies by more scholars found that IDH1 / 2 mutations, as a sign of good prognosis, commonly exist in grade II and grade III gliomas and secondary glioblastomas, and are involved in the occurrence, development and evolution of tumors. play an important role. Mutations in the IDH1 / 2 genes are the most common type of metabolic enzyme gene mutations and are quite common in gliomas and some subtypes of acute myeloid leukemia. Chondrosarcoma, cholangiocarcinoma, paraganglioma, colorectal cancer, prostate cancer, lung cancer and other solid tumors have also ...

AI Technical Summary

Problems solved by technology

At present, the methods for detecting IDH1 / 2 gene mutation mainly include chip technology, probe primer technology, enzyme digestion, pyrosequencing technology and HRM analysis technology, etc., which are expensive, cumbersome or not accurate.

Images