Artificial pollination method for accelerating manglietia glauca to fructify and generate seeds
A technology of artificial pollination and magnolia, applied in the direction of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of limited scale planting, zero seed setting rate, ovary abortion, etc., so as to facilitate the acceptance of pollen , Promote germination and increase fruit setting rate
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[0028] The above pollination method may specifically include: first, use a hand-held electric fan to blow the stigma of the pollinated flower for 2 to 4 seconds at a distance of 8 to 10 centimeters, and then spray the stigma of the pollinated flower with a spray bottle containing the mixed solution. 0.5 to 1 milliliter, and finally dip the collected pollen with a makeup pen and evenly point it on the stigma of the pollinated flower; Soak for 5 hours, irradiate with ultrasonic waves with a frequency of 20kHz for 3 minutes at intervals of 30 minutes during the soaking process, filter, take the filtrate, heat to remove ethanol, mix in glucose and boric acid, so that the concentration of glucose is 100g / L, and the concentration of boric acid is 30mg / L, and finally feed carbon dioxide until the concentration of carbon dioxide is 4g / L to obtain a mixed solution.
[0029] Table 1 Flowering stage of single flower of Magnolia argentifolius
[0030]
Embodiment 1
[0032] Select a mature ash magnolia plant on a gentle sunny or semi-sunny slope as the object of artificial pollination and flower picking; during the transition period and full flowering period of the ash magnolia, use high-branch pruning shears every day from 9:00 to 15:00 Collect single flowers in the long-closed stage, collect anthers, spread them flat on sulfuric acid paper, collect pollen after loosening, pour it into a small beaker with sulfuric acid paper, and then use it for artificial pollination; Wrap part of the pollen with sulfuric acid paper and store it in a refrigerator at 4°C for pollination the next day; select a well-developed single flower in the first flowering and long-close stages on a single pollination target as the pollination target, and take pollination single plants As the pollination material, use a cosmetic pen to dip some pollen and evenly point it on the stigma of the pollinated flowers. The number of pollinated single flowers per single plant i...
Embodiment 2
[0034] Before pollination, first use a hand-held electric fan to blow the stigma of the pollinated flower at a distance of 8-10 cm for 2-4 seconds, and then spray the stigma of the pollinated flower with a spray bottle containing the mixed solution, and the spray volume is 0.5-1 ml , all the other parameters are exactly the same as those in Example 1, and the process is also exactly the same.
[0035] Statistical embodiment 1 and embodiment 2 pollination success rate, fruit set rate, the results are shown in Table 2.
[0036] Table 2
[0037]
[0038] As can be seen from Table 2, compared with natural pollination, the pollination success rate and fruit setting rate of Example 1 are obviously improved, and the pollination method of the present invention is adopted in Example 2, and the pollination success rate and fruit setting rate will be further improved.
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