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Extraction method of radix tetrastigme hemsleyani polysaccharose

An extraction method, the technology of Cloverleaf, is applied in the extraction of Cloverleaf polysaccharides, using the bead milling method combined with cellulase to assist in the extraction of polysaccharides, which can solve the problems of low extraction rate and slow extraction speed, and achieve simple operation, The effect of reducing time cost and shortening the extraction time

Inactive Publication Date: 2018-03-30
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem mainly solved by the present invention is to establish a new extraction method for trilobite polysaccharides in view of defects such as low extraction rate and slow extraction speed in the extraction process of trilobite polysaccharides in the prior art, specifically referring to, Enzymatic Hydrolysis Combined with Bead Milling Assisted Extraction of Clover Polysaccharides

Method used

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  • Extraction method of radix tetrastigme hemsleyani polysaccharose

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A method for extracting clover polysaccharides, comprising the steps of:

[0029] Pretreatment: remove dust and other impurities on the leaves of Ivy clover in Fujian;

[0030] S1. Dry the leaves of Ivy clover, pulverize them with an ordinary pulverizer, and sieve them; the pulverization time is 12 hours; after the pulverization, pass through an 80-mesh sieve;

[0031] S2. Treat 5 parts of Clover powder and 30 parts of sterile water at 60°C for 6 hours; the parts are parts by weight;

[0032] S3. Suction filtration; concentrate for 90 minutes at a vacuum of 95kpa and a concentration temperature of 70°C to obtain a concentrated solution; add absolute ethanol 4 times the volume of the concentrated solution to the concentrated solution, and ethanol precipitation for 20 hours; finally dry at -65 ℃, the degree of vacuum is 1pa, and the drying time is 12h to obtain Clover polysaccharide.

Embodiment 2

[0034] A method for extracting clover polysaccharides, comprising the steps of:

[0035] Pretreatment: remove dust and other impurities on the leaves of Ivy clover in Fujian;

[0036] S1. Dry the leaves of Ivy clover, and pulverize them into nano-scale powder through a nano-impact mill, and the pulverization time is 12 hours;

[0037] S2. Treat 5 parts of Clover powder and 30 parts of sterile water at 60°C for 6 hours; the parts are parts by weight;

[0038] S3. Suction filtration; concentrate for 90 minutes at a vacuum of 95kpa and a concentration temperature of 70°C to obtain a concentrated solution; add absolute ethanol 4 times the volume of the concentrated solution to the concentrated solution, and ethanol precipitation for 20 hours; finally dry at -65 ℃, the degree of vacuum is 1pa, and the drying time is 12h to obtain Clover polysaccharide.

Embodiment 3

[0040] A method for extracting clover polysaccharides, comprising the steps of:

[0041] Pretreatment: remove dust and other impurities on the leaves of Ivy clover in Fujian;

[0042] S1. Dry the leaves of Ivy clover, and pulverize them into nano-scale powder through a nano-impact mill, and the pulverization time is 12 hours;

[0043] S2. 5 parts of Clover powder, 30 parts of sterile water, and 0.3 parts of cellulase were enzymatically treated at 55°C and pH 4.5 for 3.5 hours; the parts are all parts by weight;

[0044] S3. Inactivate the enzyme at 100°C for 10 minutes; then extract at 60°C for 7 hours; filter with suction; concentrate for 90 minutes at a vacuum of 95kpa and a concentration temperature of 70°C to obtain a concentrate; add absolute ethanol 4 times the volume of the concentrate to the concentrate , alcohol precipitation for 20 hours; finally, the trilobite polysaccharide was obtained at a drying temperature of -65°C, a vacuum of 1 Pa, and a drying time of 12 ho...

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Abstract

The invention discloses a novel method for extracting radix tetrastigme hemsleyani polysaccharose. The method comprises the steps that stem leaves of tetrastigme hemsleyani are taken, dried, and smashed through a bead milling method, then the powder is subjected to a hot water extraction, a certain amount of cellulase is added, enzyme inactivation is conducted after a period of time, suction filtration is conducted, concentration is conducted, absolute ethyl alcohol is added, and a radix tetrastigme hemsleyani polysaccharose crude product is obtained after overnight alcohol precipitation. Themethod has the advantages that operation is simple, the raw materials are easy to get, and the method is suitable for large-scale production. The grain size of the radix tetrastigme hemsleyani powderprepared through a nano impact mill reaches a nano range, the polysaccharose can be more rapidly dissolved out, in addition, under the condition that cellulase is utilized to conduct auxiliary extraction, the extraction rate of the radix tetrastigme hemsleyani polysaccharose is obviously and generally improved, the extraction speed is increased, the time cost is lowered, and the enterprise performance are improved.

Description

technical field [0001] The invention relates to the technical field of health food processing, in particular to a method for extracting polysaccharides from Clover, and more specifically, to a method for extracting polysaccharides by bead milling combined with cellulase-assisted extraction. Background technique [0002] Clover (Tetrastigma hemsleyanum Diels et Gilg) is scientifically known as trefoil bud climbing vine, also known as golden thread hanging gourd. It belongs to the herbaceous plant of Vitaceae, and is mainly distributed in southwest my country, Fujian, Anhui and other regions. The whole herb of clover can be used as medicine, which has the effects of improving immunity, anti-virus, anti-inflammatory, etc., especially for children with febrile convulsions, pneumonia, viral encephalitis and other symptoms. It is one of the commonly used Chinese herbal medicines. [0003] Clover polysaccharide is one of the main active ingredients of Clover. It is light brown in c...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 邱彬茹翌郭隆华林振宇陈国南
Owner FUZHOU UNIV
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