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A strain of Bacillus amyloliquefaciens and its application for preventing and controlling the spoilage of lotus

A technology for dissolving starch spores and spoilage diseases, which is applied in the fields of application, pest control, bacteria, etc., can solve the problems of difficult operation and lack of research in the biological control process of spoilage diseases, and achieves the effects of low cost, reduced pollution and high control efficiency.

Active Publication Date: 2020-09-11
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Due to the aquatic habit of the lotus, the biological control process of rot disease is difficult to operate, and the relevant research is relatively lacking.
The research on the use of Bacillus amyloliquefaciens to control the rot of lotus has not been reported

Method used

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  • A strain of Bacillus amyloliquefaciens and its application for preventing and controlling the spoilage of lotus
  • A strain of Bacillus amyloliquefaciens and its application for preventing and controlling the spoilage of lotus
  • A strain of Bacillus amyloliquefaciens and its application for preventing and controlling the spoilage of lotus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: Isolation and identification of Bacillus amyloliquefaciens B-36 bacterial strain

[0026] 1. Isolation of B-36 strain

[0027] The applicant isolated and screened a strain with a strong control effect on the rot of the lotus from the lotus plant of Huazhong Agricultural University, Huazhong Agricultural University, Wuhan City, Hubei Province, China, and named it B-36. The separation and purification of the B-36 bacterial strain of the present invention adopt methods such as dilution coating plate method, plate streaking, biological assay and the like. The specific method is as follows:

[0028] (1) Collect 30 lotus root tubers as materials, and clean the materials with running water. In order to ensure the cleaning effect, add detergent when cleaning, or process with ultrasonic waves when cleaning with running water, and finally rinse with sterile water;

[0029] (2) The tissue sections washed with sterile water were soaked in 75% alcohol for 5 minutes, ...

Embodiment 2

[0042] Example 2: Effect of B-36 bacterial strain on growth of Fusarium equiseti in plate

[0043] Pick a single colony of B-36 and draw two straight lines 2cm away from the center of the plate, and transfer a 0.3cm diameter agar block of Fusarium equiseti into the center of the plate at the same time, and place it in a 25°C incubator for culture, 4 times for each strain repeat. The control group was not streaked with bacteria, but was only inoculated with mycelium blocks of Fusarium equiseti. After 3 days, measure the size of the radius of the bacteriostatic zone (B-36 colony center to the edge of the pathogenic bacteria hyphae), and calculate the bacteriostatic rate.

[0044] Bacteriostatic rate=(growth rate of control Fusarium-growth rate of treated Fusarium) / growth rate of control Fusarium×100%.

[0045] Effect of Table 1B-36 on the Growth of Fusarium Equisetum in Plates (3d, 25°C)

[0046]

[0047] The results of the B-36 bacterial strain on the growth of Fusarium e...

Embodiment 3

[0048] Embodiment 3: the influence of B-36 bacterial strain fermentation supernatant on the growth of Fusarium equiseti

[0049] Mix the sterile filtrate of B-36 with the appropriate temperature PDA medium at a volume ratio (V / V) of 1%, 5%, 10%, and 20% respectively to make a PDA plate, and inoculate the activated Fusarium equisetia Bacterial hyphae blocks were cultured in the dark at 25°C for observation. After 7 days, the diameter of the Fusarium colony was measured by the cross method and the inhibition rate was calculated. Fusarium equisetia was inoculated on PDA medium as a control.

[0050] Effect of table 2B-36 fermentation supernatant on the growth of Fusarium equisetia (7d, 25°C)

[0051]

[0052] The experimental results of the effect of B-36 strain fermentation supernatant on the growth of Fusarium equiseti showed that different concentrations of B-36 strain fermentation supernatant had a certain inhibitory effect on the growth of Fusarium equiseti. As the conc...

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Abstract

The invention discloses a strain of Bacillus amyloliquefaciens for preventing and treating spoilage disease of lotus and its application. The Bacillus amyloliquefaciens is ( Bacillus amyloliquefaciens ) B‑36, deposit number: CCTCC NO: M 2017652. In the indoor plate confrontation test, the growth inhibition rate of the B-36 strain to Fusarium reached 74.07%; the growth inhibition rate of the B-36 fermentation broth with a concentration of 20% was as high as 90.46%; the results of the indoor pot test showed that , the leaf diameter and leaf height of B‑36 bacterial suspension treatment group were significantly increased compared with the blank control, and the control effect of seed lotus rot in the late growth stage of seed lotus could reach 46.20%, so this strain is a strain with great potential for development Bio-control bacteria for the prevention and treatment of seed lotus rot disease.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to a bacillus amyloliquefaciens strain for preventing and treating rot disease of lotus seedlings and its application. Background technique [0002] Seed lotus is a common aquatic vegetable. Lotus seeds are sweet and delicious when eaten fresh, and they can also be made into a variety of dishes. They are a common ingredient. In addition, lotus seeds, lotus hearts, lotus leaves, lotus flowers, etc. all have a variety of health care and medicinal functions. Therefore, the rapid development of the lotus industry in recent years has become a new bright spot for farmers to increase their income. In the production of lotus seeds, the continuous use of lotus ponds has created conditions for the occurrence of rot caused by pathogenic bacteria infecting lotus seeds. Due to the residues of lotus root joints and other residues, pathogenic bacteria acc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00C12R1/07
CPCA01N63/00A01N63/10C12N1/205C12R2001/07
Inventor 肖炎农张臻孟俊峰王高峰肖雪琼
Owner HUAZHONG AGRI UNIV
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