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Cytokine combination for cytokine storm monitoring and detection method

A cytokine and detection method technology, applied in the field of biological diagnosis, to achieve the effect of improving accuracy, good specificity and high detection throughput

Inactive Publication Date: 2018-04-03
WAYEN BIOTECHNOLGIES SHANGHAI INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Based on this, it is necessary to provide a method that can be used for cytokine storm monitoring for the problem of cytokine storm monitoring

Method used

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  • Cytokine combination for cytokine storm monitoring and detection method
  • Cytokine combination for cytokine storm monitoring and detection method
  • Cytokine combination for cytokine storm monitoring and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: In this example, various reagent consumables and specific experimental procedures included are as follows:

[0021] 1. Solid-phase chip for cytokine storm monitoring

[0022] · Including chips for detecting IFN-γ, TNF-α, IL-2R, IL-6, IL-10, GM-CSF, CRP, MIP-1α, MIP-1β indicators, and antibodies that capture the indicators are spot-made on the chip;

[0023] 9 kinds of biotin-labeled detection antibodies corresponding to IFN-γ, TNF-α, IL-2R, IL-6, IL-10, GM-CSF, CRP, MIP-1α, MIP-1β;

[0024] ·Streptavidin-fluorescence (SA-Cy3);

[0025] ·Supporting reagents: standard mixture, sample diluent, blocking solution, eluent I, eluent II;

[0026] ·Other equipment consumables: centrifuge tube, 4℃ centrifuge, room temperature centrifuge, microplate reader, vortex shaker, shaker, chip mini centrifuge, chip scanner, software GenePix Pro 6.0.

[0027] 2. Using a solid-phase chip to detect the 9 relevant index combinations of the present invention

[0028] (1) Preparation before chip e...

Embodiment 2

[0072] Embodiment 2: In this embodiment, various reagent consumables and specific experimental steps included are as follows:

[0073] 1. Liquid-phase chip for cytokine storm monitoring

[0074] ·Includes microspheres coated with IFN-γ, TNF-α, IL-2R, IL-6, IL-10, GM-CSF, CRP, MIP-1α, MIP-1β capture antibody, each of which has Different color coding;

[0075] 9 kinds of biotin-labeled detection antibodies corresponding to IFN-γ, TNF-α, IL-2R, IL-6, IL-10, GM-CSF, CRP, MIP-1α, MIP-1β;

[0076] ·Streptavidin-phycoerythrin (SA-PE);

[0077] ·Other supporting reagents: sample / standard product diluent, analysis buffer, antibody diluent, eluent;

[0078] ·Other equipment consumables: shaker, magnetic plate washer, oscillator, sealing film, scanner.

[0079] 2. Use the liquid phase chip to detect the combination of 9 relevant indicators of the present invention

[0080] (1) Preparation before chip experiment

[0081] 1. Take out all reagents from the -80℃ refrigerator in advance, and place them to...

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PUM

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Abstract

The invention relates to detection of cytokines related to cytokine storm and provides an index combination used for executing immunization therapy or prognosis detection for other diseases producingcytokine storm. The cytokine combination covers 9 indexes capable of detecting the cytokine storm and can realize high-sensitivity detection of a serum micro-sample with a solid phase chip / liquid phase chip / ELISA (enzyme linked immunosorbent assay) kit method. The cytokine combination has the characteristics of good focusing property, high accuracy and the like in the cytokine storm monitoring process, can guide diagnosis and treatment and has remarkable clinical application value.

Description

Technical field [0001] The invention relates to a combination of indicators for detecting cytokine storms, in particular to detection by a solid phase chip / liquid phase chip / ELISA kit method, and belongs to the field of biological diagnosis. Background technique [0002] In many diseases, including some cases of influenza, sepsis or immunotherapy, a special type of clinical syndrome may occur, that is, accompanied by fever, hypotension, hypoxia, and rapid mass production of multiple cytokines in body fluids. This series The clinical and experimental symptoms are called cytokine release syndrome, also known as cytokine storm. [0003] Cytokines related to cytokine storm are released when immune cells are activated and interact with target cells. Related cytokines (interleukins, interferons, etc.) are mainly derived from activated immune cells, and re-act on the activation of immune cells (including T-cells, monocytes and macrophages, etc.), triggering acute inflammation and inducin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/531
CPCG01N33/53G01N33/531
Inventor 张庆华匡鑫宋凯
Owner WAYEN BIOTECHNOLGIES SHANGHAI INC
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