The invention discloses a bearing base horizontal positioning mechanism, and relates to the technical field of bearing base auxiliary devices. The mechanism comprises a mounting base, a lead screw, amoving part, an adjusting part, a bearing base, a screw rod, a fixed piece, a movable part, a support part and elastic parts. Through ejection of the lead screw or pulling of the bearing base to moveup and down, the adjustment of the distance from the mounting base is realized, the mounting levelness of the bearing base can be precisely positioned, and the later levelness adjustment can be realized; through movable connection of the screw rod, the movable part and the support part and the elastic parts, the force unloading and returning work during pressed deflection of the left and right ends of the bearing base is realized, so that the bearing base is always in a transverse horizontal position; and when the bearing base is deflected, the elastic parts at the left and right ends both canwork, so that the elastic parts can be suffered from the lowest deformation force, the excessive deflection of the bearing base is prevented, the final position precision after adjustment of the deflection of the bearing base is guaranteed, and the adjustment effect achieves the ideal state.