Preparation method and application of monodisperse polymer fluorescent microsphere

A technology of fluorescent microspheres and polymers, applied in chemical instruments and methods, luminescent materials, etc., can solve the problems of cumbersome preparation methods and so on

Active Publication Date: 2018-04-06
上海艾瑞德生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few reports on the preparation of fluorescent microspheres with stable fluorescence performance, contro

Method used

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  • Preparation method and application of monodisperse polymer fluorescent microsphere
  • Preparation method and application of monodisperse polymer fluorescent microsphere
  • Preparation method and application of monodisperse polymer fluorescent microsphere

Examples

Experimental program
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Example Embodiment

[0038] (1) Preparation of oil phase mixture: adding functional reactive monomer, fluorescent molecule and reactive monomer styrene into superhydrophobic agent n-hexadecane, and ultrasonically mixing evenly, it is an oil phase mixture;

[0039] (2) Preparation of aqueous phase mixture: take a certain amount of surfactant and disperse it in water, the total volume is 9 mL, and ultrasonically treat it to completely dissolve it, which is an aqueous phase mixture;

[0040] (3) Initiator aqueous solution preparation: add the initiator to 1 mL of water, shake to dissolve;

[0041] (4) Emulsification process: add the water phase to the oil phase, stir and ultrasonically carry out pre-emulsification, and then treat with a cell pulverizer for 10 minutes to complete emulsification under certain power and ice bath conditions;

[0042] (5) transfer the above-mentioned emulsion into a 100mL three-necked flask, add the initiator aqueous solution prepared in (3), stir and mix well, heat up to...

Example Embodiment

[0053] Example 1:

[0054] Effect of Surfactant Addition on Particle Size and Dispersibility of Polymer Microspheres

[0055] (1) Preparation of oil phase mixture: Weigh 1.8g of styrene and 0.2g of acrylic acid and add them to 0.1g of n-hexadecane, a superhydrophobic agent, and mix them uniformly by ultrasonic to obtain an oil phase mixture;

[0056] (2) Preparation of aqueous phase mixture: Disperse 0.002g (0.1wt%) or 0.01g (0.5wt%) or 0.02g (1wt%) of surfactant sodium dodecyl sulfate (SDS) in water respectively, The total volume is 9mL, which is completely dissolved by shaking and is an aqueous phase mixture;

[0057] (3) Initiator aqueous solution preparation: weigh 0.02g potassium persulfate and add it to 1mL water, shake and dissolve;

[0058] (4) Emulsification: add the water phase to the oil phase, stir or ultrasonically carry out pre-emulsification, and then treat with a cell crusher for 10 min under the condition of 250W power and ice bath to complete emulsification...

Example Embodiment

[0061] Example 2:

[0062] The preparation of carboxyl-functionalized polymer fluorescent microspheres includes the following steps:

[0063] (1) Preparation of oil phase mixture: Weigh 1.8 g of styrene, 0.2 g of acrylic acid and 0.01 g (0.5 wt %) of naphthalimide fluorescent molecules into 0.1 g of n-hexadecane, a superhydrophobic agent, and mixed by ultrasonic Homogeneous, oil phase mixture;

[0064] (2) the preparation of water phase mixture: get the surfactant sodium lauryl sulfate of 0.005g and disperse in water, total volume is 9mL, shake and dissolve completely, it is water phase mixture;

[0065] (3) Initiator aqueous solution preparation: Weigh 0.02g potassium persulfate and add it to 1mL water, shake it to dissolve;

[0066] (4) Emulsification process: add the water phase to the oil phase, stir or ultrasonically carry out pre-emulsification, and then treat with a cell crusher for 10 minutes under the condition of 250W power and ice bath to complete emulsification; ...

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Abstract

The invention discloses a preparation method and application of a monodisperse polymer fluorescent microsphere. The invention provides a method for obtaining a functionalized fluorescent microsphere with a surface containing a carboxyl group or an amino group through a one-step method. The method comprises the steps of (1) providing an oil phase mixture, a water phase mixture and an initiator water solution; (2) fully mixing the water phase mixture and the oil phase mixture, pre-emulsifying and completely emulsifying to obtain an emulsion for standby use; (3) uniformly mixing the emulsion forstandby use and the initiator water solution; carrying out polymerization reaction under nitrogen protection and dark conditions, and purifying after finishing reaction, thus obtaining the polymer fluorescent microsphere. The preparation method provided by the invention is simple, the process is environmentally-friendly, and the cost is low; the obtained fluorescent microsphere is controllable inparticle size, uniform in size, high in fluorescent brightness, high in functional group content, and stable in performance, and has a better application prospect in immune side chromatography.

Description

technical field [0001] The invention relates to the fields of polymer nanocomposite materials and medical detection, in particular to a preparation method of polymer fluorescent microspheres and its application in immunological lateral chromatography. Background technique [0002] Fluorescent microspheres generally refer to microspheres with sizes ranging from nanometers to micrometers, and loaded with fluorescent substances on the surface or inside, which can excite fluorescence under the stimulation of external energy. Due to the stable morphological structure, good fluorescence characteristics and high fluorescence sensitivity of fluorescent microspheres, they have a very wide range of applications in the field of biomedicine. Fluorescence immunochromatography technology uses the active functional groups on the surface of fluorescent microspheres to bind to antibodies, which can realize the detection of the antigen to be tested in the blood. This technology can achieve a...

Claims

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Application Information

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IPC IPC(8): C09K11/06C08F212/08C08F220/06C08J3/12
CPCC08F212/08C08J3/12C08J2325/08C09K11/06C08F220/06
Inventor 操凤文陈凯敏麻玉雯瞿祎袁望董志茂李博学王佩瑞叶一肖琨倪晓涛陆亮朱轩仪杨茜茹李秋萍李欢曹秋岑
Owner 上海艾瑞德生物科技有限公司
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