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Caprine herpesvirus I vaccine strain and application thereof

A technology of herpes virus and vaccine strains, applied in the direction of antiviral agents, viruses/phages, medical preparations containing active ingredients, etc., to achieve strong pathogenicity, reduce morbidity, and stabilize biological characteristics

Active Publication Date: 2018-04-10
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no vaccine to prevent goat herpesvirus type Ⅰ infection in my country

Method used

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  • Caprine herpesvirus I vaccine strain and application thereof
  • Caprine herpesvirus I vaccine strain and application thereof
  • Caprine herpesvirus I vaccine strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Isolation and Identification of Goat Herpesvirus Type Ⅰ JSHA1405 Strain

[0019] 1. In situ samples and cells

[0020] In 2014, large-scale goat farms in Hai'an County, Jiangsu Province and other places in my country successively appeared diseases with respiratory symptoms as the main manifestations, manifested as depression, panting, coughing, serous fluid or thick nasal fluid. The nasal swabs of the affected goats were collected and stored at -80°C. Bovine kidney passage cells (MDBK) were purchased from China Veterinary Drug Administration.

[0021] 2. Virus isolation and identification

[0022] 2.1 Isolation and purification of virus

[0023] The nasal swab samples collected from the diseased goats were sterilized by filtration through a 0.22 μm filter membrane, inoculated in MDBK monolayer cell culture, and incubated at 37°C, 5% CO 2 The cells were cultured in the dark in the incubator, and the cytopathic changes (CPE) were observed every day. When bl...

Embodiment 2

[0037] The immune effect test of embodiment 2 goat herpes virus type Ⅰ inactivated vaccine (JSHA1405 strain)

[0038] 1. Poison species

[0039] The virus seed used for making seedlings is goat herpesvirus type Ⅰ JSHA1405 strain, which has been preserved in the China Center for Type Culture Collection. The preservation information is as follows:

[0040] Taxonomic name: goat herpesvirus type Ⅰ JSHA1405 strain

[0041] Caprine Herpesvirus 1JSHA1405;

[0042] Address: Wuhan University, Wuhan, China, zip code 430072;

[0043] Deposit number: CCTCC NO: V201745;

[0044] Deposit date: October 24, 2017.

[0045] 2. Preparation of poisonous seeds

[0046] Cultivate MDBK cells until the confluence reaches 70%, remove the culture medium, inoculate goat herpesvirus type I JSHA1405 strain, add cell maintenance medium (DMEM medium containing 2% fetal bovine serum), and incubate at 37°C, 5% CO 2 Culture in the dark in the cell culture box, collect the cell culture 48 hours after inoc...

Embodiment 3

[0059] Example 3 Safety Test of Goat Herpes Virus Type Ⅰ Inactivated Vaccine (JSHA1405 Strain)

[0060] 1. Experimental vaccines

[0061] According to the method in Example 2, 3 batches of goat herpes virus type I inactivated vaccines were prepared in the laboratory, and the batch numbers were: SZ-001, SZ-002, SZ-003, 500 heads / bottle, 2-8 Store at ℃.

[0062] 2. Experimental animals

[0063] Healthy pregnant ewes and 2-3-month-old healthy lambs negative for CpHV-1 antigen and antibody were purchased from a goat farm in Jurong City, Jiangsu Province.

[0064] 3. Lamb safety test

[0065] 3.1 Grouping and scheme: 50 lambs aged 2-3 months were randomly divided into 4 groups (single dose group, double dose group, single dose repeat group and control group), of which single dose group, double dose group 1. There were 15 rats in each group in the single-dose repeated group, which were divided into 3 different procedures for immunization, and 5 rats in the control group. Single...

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Abstract

The invention provides a caprine herpesvirus I vaccine strain and an application thereof, and relates to the field of biological veterinary products. The caprine herpesvirus I vaccine strain is a caprine herpesvirus I JSHA1405 strain, which is preserved with preservation number of CCTCC NO:V201745. Virus liquid is obtained through multiplication of the virus (caprine herpesvirus I) by virtue of mardin-darby bovine kidney cells. The invention also provides an application fot eh vaccine strain in preparing caprine herpesvirus I vaccines. The caprine herpesvirus I JSHA1405 strain provided by theinvention is relatively strong in pathogenicity and is good in immunogenicity. An oil adjuvant inactivated vaccine, which is prepared by the caprine herpesvirus I JSHA1405 strain, is safe and reliable; goats, which are immunized, can generate an antibody at a relatively high level, and the duration of the antibody can reach 6 months; in addition, an excellent protective effect can be taken on homologous virus seed toxicity attack; morbidity in the immunized goats is obviously reduced; and the prevalence of the caprine herpesvirus I can be effectively prevented.

Description

technical field [0001] The invention relates to the field of veterinary biological products, in particular to a goat herpes virus type I vaccine strain and its application. Background technique [0002] Caprine herpesvirus type 1 (Caprine herpesvirus 1, CpHV-1) is a double-stranded DNA virus belonging to the varicellavirus genus of the herpesviridae subfamily of the family Herpesviridae. Infection of adult goats with this virus presents as subclinical infection, resulting in a variety of symptoms including respiratory illness, fever and leukopenia, vulvovaginitis and balanitis. Infection can induce abortion in goats that are 3 to 4 months pregnant. Neonates present with severe infection, with main symptoms of fever, conjunctivitis, increased ocular discharge, dyspnea, intestinal ulceration, and necrotic lesions, accompanied by high morbidity and mortality. In 1974, CpHV-1 was first isolated from the diseased feed of newborn lambs with enteritis in California, USA. Since th...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/245A61P31/22C12R1/93
Inventor 郝飞李文良毛立李基棕刘茂军
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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