High-flux micro-fluidic chip for detecting pathogeny of epidemic disease of respiratory tract of pigs and detection method

A microfluidic chip and detection chip technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of complex pathogenic procedures and complex pathogenic factors, and achieve rapid detection, sensitivity High and cost-reducing effect

Inactive Publication Date: 2019-09-10
GUANGXI VETERINARY RES INST
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  • Abstract
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Problems solved by technology

The main respiratory diseases of pigs include swine fever, porcine reproductive and respiratory syndrome, porcine circovirus disease, Haemophilus parasuis, swine panting disease, porcine lung disease, swine flu, etc. The pathogenic fac

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  • High-flux micro-fluidic chip for detecting pathogeny of epidemic disease of respiratory tract of pigs and detection method
  • High-flux micro-fluidic chip for detecting pathogeny of epidemic disease of respiratory tract of pigs and detection method
  • High-flux micro-fluidic chip for detecting pathogeny of epidemic disease of respiratory tract of pigs and detection method

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Embodiment Construction

[0075] The present invention will be described in further detail below in conjunction with specific examples, but the embodiments of the present invention are not limited to the scope indicated by the examples. These examples are only used to illustrate the present invention, not to limit the scope of the present invention. In addition, after reading the content of the present invention, those skilled in the art can make various modifications to the present invention, and these equivalent changes also fall within the scope defined by the appended claims of the present invention. The experimental methods used in the following examples are conventional methods unless otherwise specified. Materials, reagents, etc. used in the following examples, if no special instructions, can be obtained from commercial sources

[0076] 1. Materials and methods

[0077] 1) Main reagents

[0078] Viral DNA / RNA genome extraction kits and bacterial genome DNA extraction kits were purchased from ...

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Abstract

The invention discloses a high-flux micro-fluidic chip for detecting pathogeny of the epidemic disease of the respiratory tract of pigs and a detection method and belongs to the technical field of biological chips. The high-flux micro-fluidic chip comprises an LAMP amplification reagent, a DNA/cDNA template, ddH2O, LAMP positive control, LAMP negative control, a detection chip, a chip probe and amicro-fluidic chip analyzer, the detection chip is placed in the constant-temperature amplification micro-fluidic chip analyzer, and the chip probe comprises nucleotide sequences shown in SEQ ID NO:1-42 and specific nucleic acid segments applied to detection of the hog cholera virus, porcine reproductive and respiratory syndrome virus, porcine circovirus 2 type, porcine circovirus 3 type, haemophilus parasuis, mycoplasma hyopneumoniae, porcine pasteurella and swine influenza virus. According to the high-flux micro-fluidic chip, detection can be completed within one hour, the detection limit is5*10-2ng/microliter, different kinds of pathogeny of the epidemic disease are accurately distinguished, and the high-flux micro-fluidic chip has the advantages of quick detection, high sensitivity and accurate specificity.

Description

technical field [0001] The invention relates to the technical field of biochips, in particular to a high-throughput microfluidic chip and a detection method for detecting porcine respiratory disease pathogens. Background technique [0002] In recent years, porcine respiratory disease is one of the most harmful and widespread important common diseases to the pig industry, seriously endangering the development of the pig industry. The main respiratory diseases of pigs include swine fever, porcine reproductive and respiratory syndrome, porcine circovirus disease, Haemophilus parasuis, swine panting disease, porcine lung disease, swine flu, etc. The pathogenic factors are complex, and generally mixed infection The existence of the form of secondary infection and secondary infection makes the clinical detection of the pathogen complicated, and multiple detections are required to make a correct judgment. [0003] Microfluidic chip technology can integrate the sample detection pro...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/689C12Q1/6844C12N15/11C12R1/93C12R1/35C12R1/01
CPCC12Q1/6844C12Q1/689C12Q1/701C12Q2531/119C12Q2563/107C12Q2565/629
Inventor 潘艳李军贺会利彭昊冯世文钟舒红胡帅马春霞李常挺谢永平
Owner GUANGXI VETERINARY RES INST
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