Watermelon pollen preservation method and watermelon pollen activation method

A watermelon pollen and preservation method technology, applied in the direction of plant preservation, botanical equipment and methods, applications, etc., can solve the problem of male flower pollen losing vitality, etc., and achieve the effects of improving preservation effect, high fruit setting rate, and improving vitality

Inactive Publication Date: 2018-04-13
芜湖县海琦生态农业有限公司
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  • Abstract
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Problems solved by technology

However, if the flowering period encounters low temperature and rainy days, although the wa

Method used

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Embodiment Construction

[0018] A kind of preservation method of watermelon pollen, comprises the following steps:

[0019] (1) Pollen collection and processing:

[0020] During the flowering period from 3:00 p.m. to 5:00 p.m., collect the watermelon male flower buds that are about to open, peel off the petals and sepals, put them in a paper box, and then put them in a desiccator to dry, wait for loose powder, and collect pollen after the powder is released;

[0021] (2) Pre-cooling treatment:

[0022] The collected pollen was vacuum-packed, pre-cooled in a refrigerator at 3°C ​​for 2 hours, and then quickly frozen with liquid nitrogen and stored in a low-temperature refrigerator at -18°C.

[0023] Wherein, in the step (1), set the temperature of the dryer at 28° C. and dry for 2 hours.

[0024] Wherein, when the pollen is preserved, an appropriate amount of glycerin is also added as a cryoprotectant.

[0025] The activation method of the watermelon pollen obtained by using the preservation method,...

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Abstract

The invention discloses a watermelon pollen preservation method. The watermelon pollen preservation method comprises the following steps: pre-cooling fresh pollen at first; then performing quick freezing treatment to greatly reduce the water content of pollen; and meanwhile, adding glycerinum in the preservation period to ensure that formation of ice crystals in pollen cells in the vacuum preservation period is reduced, so that breakage of the pollen cells is reduced, the vitality after freezing is improved, and the watermelon pollen preservation effect is improved. The invention also discloses an activation method for frozen-preserved pollen, unfreezing water absorption and radioactivation are performed on the frozen-preserved pollen, vitality maintaining of pollen is facilitated, a highfruit set rate can be kept after pollination, formation of a haploid can be induced after gamma ray radiation, and cultivation of a seedless watermelon is facilitated.

Description

technical field [0001] The invention belongs to the technical field of facility horticulture production, and in particular relates to a method for preserving watermelon pollen and a method for activating the corresponding pollen. Background technique [0002] In the natural environment, the lifespan of watermelon pollen is very short, usually only a few hours. In the afternoon of the flowering day, the corolla will wither, the stamens will shrink, and the pollen will lose its vitality. In sunny days and high temperatures, the lifespan of watermelon flowers and pollen will be shorter. However, if the flowering period encounters low temperature and rainy days, although the watermelon flowers can still open the next day, the male flower pollen has lost its vitality. Studies have shown that environmental conditions such as temperature and humidity are the main factors affecting the viability of plant pollen, and the conditions required by different plants vary greatly. [00...

Claims

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Application Information

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IPC IPC(8): A01N3/00
CPCA01N3/00
Inventor 许继跃
Owner 芜湖县海琦生态农业有限公司
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