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Preparation method and application of olive leaf extract

An olive leaf extract and olive leaf technology, applied in the direction of botanical equipment and methods, applications, skin care preparations, etc., can solve the problem of easy oxidation of oleuropein, difficult preparation of oleuropein, low product purity and yield and other problems, to achieve the effect of shortening the extraction time, improving the safety of use, and increasing the extraction rate

Inactive Publication Date: 2018-04-13
马斌祥
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many extraction methods of oleuropein that have been reported, but the purity and yield of the product are very low, and because oleuropein is easily oxidized, sensitive to temperature and light, has strong hygroscopicity, and is not easy to crystallize, so high Pure oleuropein is not easy to prepare

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Pretreatment: Harvest 10g of fresh olive leaves, inject 20g of disinfectant after washing and rinsing, the concentration of chlorine dioxide in the disinfectant is 50mg / L, so that the disinfectant can completely infiltrate the olive leaves, and keep the temperature of the disinfectant at 35-40°C, stir for 5 minutes and then stand for 5 minutes, continue to stir for 5 minutes and then stand for 5 minutes, stir again for 5 minutes and then stand for 5 minutes, drain the disinfectant, and rinse with cleaning to complete the pretreatment of olive leaves;

[0025] (2) Primary extraction: Inject 30 g of water and 0.05 g of compound enzyme into 10 g of pretreated olive leaves, keep stirring at 35-40° C., heat to reflux state after 2 hours and keep stirring for 10 minutes to obtain the olive leaf mixture; Described compound enzyme is made of Trichoderma viride and trypsin of mass ratio 2:1;

[0026] (3) Secondary extraction: add absolute ethanol to the olive leaf mixture ob...

Embodiment 2

[0029] (1) Pretreatment: Harvest 10g of fresh olive leaves, rinse and inject 20g of disinfectant solution, the concentration of the disinfectant polyhexamethyleneguanidine hydrochloride in the disinfectant solution is 50mg / L, so that the disinfectant solution can completely infiltrate the olive leaves and disinfect Keep the temperature of the solution at 35-40°C, stir for 5 minutes and then stand for 5 minutes, continue to stir for 5 minutes, then stand for 5 minutes, stir again for 5 minutes and then stand for 5 minutes, drain the disinfectant, and rinse with a washing machine to complete the pretreatment of olive leaves;

[0030] (2) Primary extraction: Inject 30 g of water and 0.05 g of compound enzyme into 10 g of pretreated olive leaves, keep stirring at 35-40° C., heat to reflux state after 2 hours and keep stirring for 10 minutes to obtain the olive leaf mixture; Described compound enzyme is made of Trichoderma viride and trypsin of mass ratio 2:1;

[0031] (3) Secondar...

Embodiment 3

[0034] (1) Pretreatment: Harvest 10g of fresh olive leaves, inject 20g of disinfectant after washing and rinsing, the concentration of disinfectant in the disinfectant is 50mg / L, so that the disinfectant can completely infiltrate the olive leaves, and the temperature of the disinfectant is kept at 35-40 ℃, stirred for 5 minutes and then stood for 5 minutes, continued to stir for 5 minutes, then stood for 5 minutes, stirred again for 5 minutes and then stood for 5 minutes, drained the disinfectant, and rinsed with cleaning, that is, the pretreatment of olive leaves was completed;

[0035] (2) Primary extraction: Inject 30 g of water and 0.05 g of compound enzyme into 10 g of pretreated olive leaves, keep stirring at 35-40° C., heat to reflux state after 2 hours and keep stirring for 10 minutes to obtain the olive leaf mixture; Described compound enzyme is made of Trichoderma viride and trypsin of mass ratio 2:1;

[0036] (3) Secondary extraction: add absolute ethanol to the oli...

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PUM

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Abstract

The invention discloses a preparation method of an olive leaf extract, and relates to the technical field of extraction of plant components. The preparation method comprises the following steps: (1) pretreatment, (2) primary extraction, (3) secondary extraction and (4) concentration and purification. In the preparation method, a two-level extraction form is adopted; in the primary extraction, cellwalls and cell membranes of olive leaves are quickly destructed by adopting a complex enzyme, so that the leaching speed of oleuropein is increased; after inactivation treatment is performed on the complex enzyme, through the secondary extraction, the leaching speed of the oleuropein is further increased, the extraction time is shortened, and the extraction rate of the oleuropein is improved.

Description

Technical field: [0001] The invention relates to the technical field of plant component extraction, in particular to a preparation method and application of olive leaf extract. Background technique: [0002] Olive is a world-renowned and precious evergreen woody oil and fruit tree species characterized by "high yield, high quality, and high efficiency". There are as many as 500 cultivars, and about 140 species are widely planted. Olives have strong growth ability, drought tolerance, and light-loving. They are mainly distributed in countries along the Mediterranean Sea in Europe and California in America, and then introduced to my country. For a long time, fresh olives have been mainly processed into olive oil known as the "Queen of Vegetable Oils". [0003] Analysis of modern biotechnology shows that olive leaves contain a large number of biologically active substances, and their antioxidant active ingredients are more prominent than olive fruits, especially split iridoids ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q19/00A61Q19/08A61L2/18A01N65/44A01N65/42A01N65/22A01N65/24A01N65/08A01P3/00A61L101/06A61L101/46
CPCA01N65/00A01N65/08A01N65/22A01N65/24A01N65/42A01N65/44A61K8/97A61K2800/805A61K2800/84A61L2/18A61Q19/005A61Q19/08
Inventor 马斌祥
Owner 马斌祥